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- W2922423146 abstract "Abstract We describe a new technique that combines ultrasound and microfluidics to rapidly size and count cells in a high-throughput and label-free fashion. Using 3D hydrodynamic flow focusing, cells are streamed single file through an ultrasound beam where ultrasound scattering events from each individual cell are acquired. The ultrasound operates at a center frequency of 375 MHz with a wavelength of 4 μm; when the ultrasound wavelength is similar to the size of a scatterer, the power spectra of the backscattered ultrasound waves have distinct features at specific frequencies that are directly related to the cell size. Our approach determines cell sizes through a comparison of these distinct spectral features with established theoretical models. We perform an analysis of two types of cells: acute myeloid leukemia cells, where 2,390 measurements resulted in a mean size of 10.0 ± 1.7 μm, and HT29 colorectal cancer cells, where 1,955 measurements resulted in a mean size of 15.0 ± 2.3 μm. These results and histogram distributions agree very well with those measured from a Coulter Counter Multisizer 4. Our technique is the first to combine ultrasound and microfluidics to determine the cell size with the potential for multi-parameter cellular characterization using fluorescence, light scattering and quantitative photoacoustic techniques." @default.
- W2922423146 created "2019-03-22" @default.
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- W2922423146 date "2019-03-18" @default.
- W2922423146 modified "2023-10-15" @default.
- W2922423146 title "Sizing biological cells using a microfluidic acoustic flow cytometer" @default.
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- W2922423146 doi "https://doi.org/10.1038/s41598-019-40895-x" @default.
- W2922423146 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6423196" @default.
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