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• W2922948832 abstract "The ability of Mycobacterium tuberculosis to infect, proliferate, and survive during long periods in the human lungs largely depends on the rigorous control of gene expression. Transcriptome-wide analyses are key to understanding gene regulation on a global scale. Here, we combine 5’-end-directed libraries with RNAseq expression libraries to gain insight into the transcriptome organization and post-transcriptional mRNA cleavage landscape in mycobacteria during log phase growth and under hypoxia, a physiologically relevant stress condition. Using the model organism Mycobacterium smegmatis, we identified 6,090 transcription start sites (TSSs) with high confidence during log phase growth, of which 67% were categorized as primary TSSs for annotated genes, and the remaining were classified as internal, antisense or orphan, according to their genomic context. Interestingly, over 25% of the RNA transcripts lack a leader sequence, and of the coding sequences that do have leaders, 53% lack a strong consensus Shine-Dalgarno site. This indicates that like M. tuberculosis, M. smegmatis can initiate translation through multiple mechanisms. Our approach also allowed us to identify over 3,000 RNA cleavage sites, which occur at a novel sequence motif. To our knowledge, this represents the first report of a transcriptome-wide RNA cleavage site map in mycobacteria. The cleavage sites show a positional bias toward mRNA regulatory regions, highlighting the importance of post-transcriptional regulation in gene expression. We show that in low oxygen, a condition associated with the host environment during infection, mycobacteria change their transcriptomic profiles and endonucleolytic RNA cleavage is markedly reduced, suggesting a mechanistic explanation for previous reports of increased mRNA half-lives in response to stress. In addition, a number of TSSs were triggered in hypoxia, 56 of which contain the binding motif for the sigma factor SigF in their promoter regions. This suggests that SigF makes direct contributions to transcriptomic remodeling in hypoxia-challenged mycobacteria. Taken together, our data provide a foundation for further study of both transcriptional and posttranscriptional regulation in mycobacteria." @default.
• W2922948832 created "2019-04-01" @default.
• W2922948832 creator A5042859565 @default.
• W2922948832 creator A5071490357 @default.
• W2922948832 creator A5083049598 @default.
• W2922948832 creator A5083302085 @default.
• W2922948832 date "2019-03-26" @default.
• W2922948832 modified "2023-10-10" @default.
• W2922948832 title "Defining the Transcriptional and Post-transcriptional Landscapes of Mycobacterium smegmatis in Aerobic Growth and Hypoxia" @default.
• W2922948832 cites W158371559 @default.
• W2922948832 cites W1709815505 @default.
• W2922948832 cites W1735309556 @default.
• W2922948832 cites W1776579550 @default.
• W2922948832 cites W1792698462 @default.
• W2922948832 cites W1824912594 @default.
• W2922948832 cites W1857957808 @default.
• W2922948832 cites W1895980205 @default.
• W2922948832 cites W1897533110 @default.
• W2922948832 cites W1899647260 @default.
• W2922948832 cites W1945781696 @default.
• W2922948832 cites W1968698890 @default.
• W2922948832 cites W1986910962 @default.
• W2922948832 cites W1989410164 @default.
• W2922948832 cites W1989485249 @default.
• W2922948832 cites W1996638297 @default.
• W2922948832 cites W2002160803 @default.
• W2922948832 cites W2005890154 @default.
• W2922948832 cites W2006072321 @default.
• W2922948832 cites W2010466313 @default.
• W2922948832 cites W2023401038 @default.
• W2922948832 cites W2026646185 @default.
• W2922948832 cites W2032849894 @default.
• W2922948832 cites W2033837725 @default.
• W2922948832 cites W2040694642 @default.
• W2922948832 cites W2044246903 @default.
• W2922948832 cites W2048964173 @default.
• W2922948832 cites W2049569709 @default.
• W2922948832 cites W2054522148 @default.
• W2922948832 cites W2062896692 @default.
• W2922948832 cites W2063205723 @default.
• W2922948832 cites W2065637677 @default.
• W2922948832 cites W2067540509 @default.
• W2922948832 cites W2067576392 @default.
• W2922948832 cites W2067872411 @default.
• W2922948832 cites W2068442425 @default.
• W2922948832 cites W2078358905 @default.
• W2922948832 cites W2080354196 @default.
• W2922948832 cites W2081589091 @default.
• W2922948832 cites W2082434748 @default.
• W2922948832 cites W2088139692 @default.
• W2922948832 cites W2091161501 @default.
• W2922948832 cites W2093612041 @default.
• W2922948832 cites W2093730523 @default.
• W2922948832 cites W2094222845 @default.
• W2922948832 cites W2097448738 @default.
• W2922948832 cites W2098109039 @default.
• W2922948832 cites W2098732312 @default.
• W2922948832 cites W2099910213 @default.
• W2922948832 cites W2101515608 @default.
• W2922948832 cites W2102229358 @default.
• W2922948832 cites W2102986104 @default.
• W2922948832 cites W2103314018 @default.
• W2922948832 cites W2103441770 @default.
• W2922948832 cites W2103680912 @default.
• W2922948832 cites W2103787967 @default.
• W2922948832 cites W2106767919 @default.
• W2922948832 cites W2107443178 @default.
• W2922948832 cites W2109867978 @default.
• W2922948832 cites W2110025089 @default.
• W2922948832 cites W2110088612 @default.
• W2922948832 cites W2110728874 @default.
• W2922948832 cites W2111241979 @default.
• W2922948832 cites W2114401611 @default.
• W2922948832 cites W2119794055 @default.
• W2922948832 cites W2120842435 @default.
• W2922948832 cites W2123084649 @default.
• W2922948832 cites W2123255736 @default.
• W2922948832 cites W2124080033 @default.
• W2922948832 cites W2127447446 @default.
• W2922948832 cites W2128169214 @default.
• W2922948832 cites W2128482141 @default.
• W2922948832 cites W2129957930 @default.
• W2922948832 cites W2130764574 @default.
• W2922948832 cites W2133130714 @default.
• W2922948832 cites W2133732582 @default.
• W2922948832 cites W2135724552 @default.
• W2922948832 cites W2136298033 @default.
• W2922948832 cites W2137390416 @default.
• W2922948832 cites W2140354550 @default.
• W2922948832 cites W2142136274 @default.
• W2922948832 cites W2142284828 @default.
• W2922948832 cites W2142926390 @default.
• W2922948832 cites W2150561721 @default.
• W2922948832 cites W2154662371 @default.
• W2922948832 cites W2155347381 @default.
• W2922948832 cites W2155383369 @default.
• W2922948832 cites W2156320594 @default.
• W2922948832 cites W2157468187 @default.
• W2922948832 cites W2158266834 @default.
• W2922948832 cites W2162198899 @default.

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