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• W2923139412 abstract "Streptococcus pneumoniae is a major cause of community acquired pneumonia and septicaemia in humans. These diseases are frequently associated with thromboembolic cardiovascular complications. Pneumococci induce the exocytosis of endothelial Weibel-Palade Bodies and thereby actively stimulate the release of von Willebrand factor (VWF), which is an essential glycoprotein of the vascular haemostasis. Both, the pneumococcus induced pulmonary inflammation and the thromboembolytic complications are characterized by a dysbalanced haemostasis including a markedly increase in VWF plasma concentrations. Here, we describe for the first time VWF as a novel interaction partner of capsulated and non-encapsulated pneumococci. Moreover, cell culture infection analyses with primary endothelial cells characterized VWF as bridging molecule that mediates bacterial adherence to endothelial cells in a heparin-sensitive manner. Due to the mechanoresponsive changes of the VWF protein conformation and multimerization status, which occur in the blood stream, we used a microfluidic pump system to generate shear flow-induced multimeric VWF strings on endothelial cell surfaces and analysed attachment of RFP-expressing pneumococci in flow. By applying immuno fluorescence visualization and additional electron microscopy, we detected a frequent and enduring bacterial attachment to the VWF strings. Bacterial attachment to the endothelium was confirmed in vivo using a zebrafish infection model, which is described in many reports and acknowledged as suitable model to study haemostasis mechanisms and protein interactions of coagulation factors. Notably, we visualized the recruitment of zebrafish-derived VWF to the surface of pneumococci circulating in the blood stream and detected a VWF-dependent formation of bacterial aggregates within the vasculature of infected zebrafish larvae. Furthermore, we identified the surface-exposed bacterial enolase as pneumococcal VWF binding protein, which interacts with the VWF domain A1 and determined the binding kinetics by surface plasmon resonance. Subsequent epitope mapping using an enolase peptide array indicates that the peptide 195YGAEIFHALKKILKS210 might serve as a possible core sequence of the VWF interaction site. In conclusion, we describe a VWF-mediated mechanism for pneumococcal anchoring within the bloodstream via surface-displayed enolase, which promotes intravascular bacterial aggregation." @default.
• W2923139412 created "2019-04-01" @default.
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• W2923139412 date "2019-03-26" @default.
• W2923139412 modified "2023-10-14" @default.
• W2923139412 title "Von Willebrand Factor Mediates Pneumococcal Aggregation and Adhesion in Blood Flow" @default.
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• W2923139412 cites W1620711825 @default.
• W2923139412 cites W1622871881 @default.
• W2923139412 cites W1665234907 @default.
• W2923139412 cites W1908623921 @default.
• W2923139412 cites W1939321567 @default.
• W2923139412 cites W1968029849 @default.
• W2923139412 cites W1976777769 @default.
• W2923139412 cites W1977696713 @default.
• W2923139412 cites W1985726492 @default.
• W2923139412 cites W1997767673 @default.
• W2923139412 cites W2000714505 @default.
• W2923139412 cites W2005522638 @default.
• W2923139412 cites W2007532386 @default.
• W2923139412 cites W2009176935 @default.
• W2923139412 cites W2011334787 @default.
• W2923139412 cites W2012030177 @default.
• W2923139412 cites W2012486050 @default.
• W2923139412 cites W2014870509 @default.
• W2923139412 cites W2022094318 @default.
• W2923139412 cites W2023989921 @default.
• W2923139412 cites W2043031406 @default.
• W2923139412 cites W2044629043 @default.
• W2923139412 cites W2047893796 @default.
• W2923139412 cites W2055212290 @default.
• W2923139412 cites W2055555815 @default.
• W2923139412 cites W2071254360 @default.
• W2923139412 cites W2075138494 @default.
• W2923139412 cites W2075549740 @default.
• W2923139412 cites W2078235464 @default.
• W2923139412 cites W2079852422 @default.
• W2923139412 cites W2080850270 @default.
• W2923139412 cites W2086007570 @default.
• W2923139412 cites W2088418877 @default.
• W2923139412 cites W2088862121 @default.
• W2923139412 cites W2093413508 @default.
• W2923139412 cites W2096087962 @default.
• W2923139412 cites W2097180636 @default.
• W2923139412 cites W2105484180 @default.
• W2923139412 cites W2106004785 @default.
• W2923139412 cites W2113626835 @default.
• W2923139412 cites W2132908707 @default.
• W2923139412 cites W2133848888 @default.
• W2923139412 cites W2137827663 @default.
• W2923139412 cites W2141477555 @default.
• W2923139412 cites W2152578576 @default.
• W2923139412 cites W2152854706 @default.
• W2923139412 cites W2167185424 @default.
• W2923139412 cites W2169722759 @default.
• W2923139412 cites W2171327947 @default.
• W2923139412 cites W2280836817 @default.
• W2923139412 cites W2292690625 @default.
• W2923139412 cites W2303190122 @default.
• W2923139412 cites W2325607383 @default.
• W2923139412 cites W2328970599 @default.
• W2923139412 cites W2344737590 @default.
• W2923139412 cites W2417359619 @default.
• W2923139412 cites W2467857529 @default.
• W2923139412 cites W2516952556 @default.
• W2923139412 cites W2568032755 @default.
• W2923139412 cites W2594888113 @default.
• W2923139412 cites W2615768521 @default.
• W2923139412 cites W2619206214 @default.
• W2923139412 cites W2729235069 @default.
• W2923139412 cites W2734261046 @default.
• W2923139412 cites W2743346725 @default.
• W2923139412 cites W2792101137 @default.
• W2923139412 cites W2794519236 @default.
• W2923139412 cites W339268589 @default.
• W2923139412 cites W4242471323 @default.
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• W2923139412 doi "https://doi.org/10.3389/fmicb.2019.00511" @default.
• W2923139412 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6443961" @default.
• W2923139412 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30972039" @default.
• W2923139412 hasPublicationYear "2019" @default.
• W2923139412 type Work @default.

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