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• W2923284916 abstract "Our method for analyzing histone modifications, scChIC-seq (single-cell chromatin immunocleavage sequencing), involves targeting of the micrococcal nuclease (MNase) to a histone mark of choice by tethering to a specific antibody. Cleaved target sites are then selectively PCR amplified. We show that scChIC-seq reliably detects H3K4me3 and H3K27me3 target sites in single human white blood cells. The resulting data are used for clustering of blood cell types. Covalent linking of a histone-modification-specific antibody to MNase allows for the isolation of fragments with the desired histone mark, which can be amplified and sequenced. This approach is sensitive enough to profile histone modifications in single cells." @default.
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• W2923284916 date "2019-03-28" @default.
• W2923284916 modified "2023-10-17" @default.
• W2923284916 title "Single-cell chromatin immunocleavage sequencing (scChIC-seq) to profile histone modification" @default.
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• W2923284916 doi "https://doi.org/10.1038/s41592-019-0361-7" @default.
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