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- W2923308436 abstract "Abstract One of the major issues in cell therapy of myocardial infarction (MI) is early death of engrafted cells in a harsh oxidative stress environment, which limits the potential therapeutic utility of this strategy in the clinical setting. Increasing evidence implicates beneficial effects of omega‐3 fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and ascorbic acid (AA) in cardiovascular diseases, in particular their role in ameliorating fibrosis. In the current study, we aim to assess the cytoprotective role of EPA + DHA and AA in protecting embryonic stem cell (ESC)‐derived cardiac lineage cells and amelioration of fibrosis. Herein, we have shown that preincubation of the cells with EPA + DHA + AA prior to H 2 O 2 treatment attenuated generation of reactive oxygen species (ROS) and enhanced cell viability. Gene expression analysis revealed that preincubation with EPA + DHA + AA followed by H 2 O 2 treatment, upregulated heme oxygenase‐1 (HO‐1) along with cardiac markers (GATA4, myosin heavy chain, α isoform [MYH6]), connexin 43 [CX43]) and attenuated oxidative stress‐induced upregulation of fibroblast markers (vimentin and collagen type 1 [Col1]). Alterations in gene expression patterns were followed by marked elevation of cardiac troponin (TNNT2) positive cells and reduced numbers of vimentin positive cells. An injection of EPA + DHA + AA‐pretreated ESC‐derived cardiac lineage cells into the ischemic myocardium of a rat model of MI significantly reduced fibrosis compared to the vehicle group. This study provided evidence that EPA + DHA + AA may be an appropriate preincubation regimen for regenerative purposes. © 2019 BioFactors, 45(3):427–438, 2019" @default.
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- W2923308436 date "2019-03-25" @default.
- W2923308436 modified "2023-10-16" @default.
- W2923308436 title "Cardioprotective effects of omega‐3 fatty acids and ascorbic acid improve regenerative capacity of embryonic stem cell‐derived cardiac lineage cells" @default.
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- W2923308436 cites W1914024321 @default.
- W2923308436 cites W1925727041 @default.
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- W2923308436 cites W1975497658 @default.
- W2923308436 cites W1979344831 @default.
- W2923308436 cites W1987197513 @default.
- W2923308436 cites W2002742846 @default.
- W2923308436 cites W2004427135 @default.
- W2923308436 cites W2009850627 @default.
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- W2923308436 cites W201398293 @default.
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- W2923308436 cites W2014587681 @default.
- W2923308436 cites W2016415187 @default.
- W2923308436 cites W2017068971 @default.
- W2923308436 cites W2017979664 @default.
- W2923308436 cites W2027953701 @default.
- W2923308436 cites W2030360639 @default.
- W2923308436 cites W2031134859 @default.
- W2923308436 cites W2035000104 @default.
- W2923308436 cites W2050747371 @default.
- W2923308436 cites W2056980068 @default.
- W2923308436 cites W2067879512 @default.
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- W2923308436 cites W2119288897 @default.
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- W2923308436 cites W2165820903 @default.
- W2923308436 cites W2177707018 @default.
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- W2923308436 cites W2328783655 @default.
- W2923308436 cites W2339538682 @default.
- W2923308436 cites W2346165911 @default.
- W2923308436 cites W2396755600 @default.
- W2923308436 cites W2463277323 @default.
- W2923308436 cites W2472899497 @default.
- W2923308436 cites W2515758893 @default.
- W2923308436 cites W2516190779 @default.
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- W2923308436 doi "https://doi.org/10.1002/biof.1501" @default.
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