Matches in SemOpenAlex for { <https://semopenalex.org/work/W2923328608> ?p ?o ?g. }
- W2923328608 abstract "Abstract Isomerases perform biotransformations without cofactors but often cause an undesirable mixture of substrate and product due to unfavorable thermodynamic equilibria. We demonstrate the feasibility of using an engineered yeast strain harboring oxidoreductase reactions to overcome the thermodynamic limit of an isomerization reaction. Specifically, a yeast strain capable of consuming lactose intracellularly is engineered to produce tagatose from lactose through three layers of manipulations. First, GAL1 coding for galactose kinase is deleted to eliminate galactose utilization. Second, heterologous xylose reductase (XR) and galactitol dehydrogenase (GDH) are introduced into the ∆gal1 strain. Third, the expression levels of XR and GDH are adjusted to maximize tagatose production. The resulting engineered yeast produces 37.69 g/L of tagatose from lactose with a tagatose and galactose ratio of 9:1 in the reaction broth. These results suggest that in vivo oxidoreaductase reactions can be employed to replace isomerases in vitro for biotransformation." @default.
- W2923328608 created "2019-04-01" @default.
- W2923328608 creator A5002871744 @default.
- W2923328608 creator A5022751421 @default.
- W2923328608 creator A5035842562 @default.
- W2923328608 creator A5036942918 @default.
- W2923328608 creator A5039117031 @default.
- W2923328608 creator A5060672032 @default.
- W2923328608 creator A5070334885 @default.
- W2923328608 creator A5078280357 @default.
- W2923328608 date "2019-03-22" @default.
- W2923328608 modified "2023-10-16" @default.
- W2923328608 title "Overcoming the thermodynamic equilibrium of an isomerization reaction through oxidoreductive reactions for biotransformation" @default.
- W2923328608 cites W1481413711 @default.
- W2923328608 cites W1771141167 @default.
- W2923328608 cites W1976215028 @default.
- W2923328608 cites W2005095483 @default.
- W2923328608 cites W2016160862 @default.
- W2923328608 cites W2033378635 @default.
- W2923328608 cites W2048108455 @default.
- W2923328608 cites W2067490649 @default.
- W2923328608 cites W2078955492 @default.
- W2923328608 cites W2080347382 @default.
- W2923328608 cites W2087089148 @default.
- W2923328608 cites W2089546919 @default.
- W2923328608 cites W2090295846 @default.
- W2923328608 cites W2090357145 @default.
- W2923328608 cites W2090422232 @default.
- W2923328608 cites W2097666362 @default.
- W2923328608 cites W2113170201 @default.
- W2923328608 cites W2115420193 @default.
- W2923328608 cites W2116672608 @default.
- W2923328608 cites W2120783001 @default.
- W2923328608 cites W2140183126 @default.
- W2923328608 cites W2147913192 @default.
- W2923328608 cites W2163679306 @default.
- W2923328608 cites W2238960245 @default.
- W2923328608 cites W2252336863 @default.
- W2923328608 cites W2258180218 @default.
- W2923328608 cites W2291301785 @default.
- W2923328608 cites W2331754424 @default.
- W2923328608 cites W2338781026 @default.
- W2923328608 cites W2411965021 @default.
- W2923328608 cites W2476717220 @default.
- W2923328608 cites W2487071432 @default.
- W2923328608 cites W2530555388 @default.
- W2923328608 cites W2730800840 @default.
- W2923328608 cites W2801136107 @default.
- W2923328608 cites W4253583001 @default.
- W2923328608 cites W79983000 @default.
- W2923328608 doi "https://doi.org/10.1038/s41467-019-09288-6" @default.
- W2923328608 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6430769" @default.
- W2923328608 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30902987" @default.
- W2923328608 hasPublicationYear "2019" @default.
- W2923328608 type Work @default.
- W2923328608 sameAs 2923328608 @default.
- W2923328608 citedByCount "26" @default.
- W2923328608 countsByYear W29233286082019 @default.
- W2923328608 countsByYear W29233286082020 @default.
- W2923328608 countsByYear W29233286082021 @default.
- W2923328608 countsByYear W29233286082022 @default.
- W2923328608 countsByYear W29233286082023 @default.
- W2923328608 crossrefType "journal-article" @default.
- W2923328608 hasAuthorship W2923328608A5002871744 @default.
- W2923328608 hasAuthorship W2923328608A5022751421 @default.
- W2923328608 hasAuthorship W2923328608A5035842562 @default.
- W2923328608 hasAuthorship W2923328608A5036942918 @default.
- W2923328608 hasAuthorship W2923328608A5039117031 @default.
- W2923328608 hasAuthorship W2923328608A5060672032 @default.
- W2923328608 hasAuthorship W2923328608A5070334885 @default.
- W2923328608 hasAuthorship W2923328608A5078280357 @default.
- W2923328608 hasBestOaLocation W29233286081 @default.
- W2923328608 hasConcept C100544194 @default.
- W2923328608 hasConcept C104317684 @default.
- W2923328608 hasConcept C181199279 @default.
- W2923328608 hasConcept C185592680 @default.
- W2923328608 hasConcept C18903297 @default.
- W2923328608 hasConcept C196493046 @default.
- W2923328608 hasConcept C197957613 @default.
- W2923328608 hasConcept C2777289219 @default.
- W2923328608 hasConcept C2778724459 @default.
- W2923328608 hasConcept C2779222958 @default.
- W2923328608 hasConcept C2779268744 @default.
- W2923328608 hasConcept C2780294150 @default.
- W2923328608 hasConcept C2781135521 @default.
- W2923328608 hasConcept C2781264208 @default.
- W2923328608 hasConcept C27881333 @default.
- W2923328608 hasConcept C547475151 @default.
- W2923328608 hasConcept C55493867 @default.
- W2923328608 hasConcept C86803240 @default.
- W2923328608 hasConceptScore W2923328608C100544194 @default.
- W2923328608 hasConceptScore W2923328608C104317684 @default.
- W2923328608 hasConceptScore W2923328608C181199279 @default.
- W2923328608 hasConceptScore W2923328608C185592680 @default.
- W2923328608 hasConceptScore W2923328608C18903297 @default.
- W2923328608 hasConceptScore W2923328608C196493046 @default.
- W2923328608 hasConceptScore W2923328608C197957613 @default.
- W2923328608 hasConceptScore W2923328608C2777289219 @default.
- W2923328608 hasConceptScore W2923328608C2778724459 @default.
- W2923328608 hasConceptScore W2923328608C2779222958 @default.