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• W2923768424 abstract "Nuclear magnetic resonance (NMR) provides unparalleled information on molecular structure and intermolecular interactions with atomic-level resolution, but it nevertheless lacks sensitivity in comparison to other forms of spectroscopy. This problem is more acute in cases where multidimensional spectra are required, such as in protein NMR structural studies and in metabolomic experiments where biomarkers of interest may be present in low concentrations. An approach to addressing the issue of sensitivity is dynamic nuclear polarization (DNP) in which NMR signal intensities are enhanced by transferring the large Boltzmann polarization of electrons to nuclear spins via microwave irradiation at the electron Larmor frequency. This article describes some recent technological innovations—in particular, the development of high-frequency gyrotron oscillators and amplifiers and novel polarizing agents—that enable applications of DNP at the 10–20 T fields where contemporary NMR experiments are performed. In addition, we discuss potential and current applications which are wide ranging, and include magic angle spinning (MAS) NMR studies of amyloid fibrils and membrane proteins, and melting and dissolution experiments applicable to solution NMR that could serve as a tool for high throughput small molecule analysis such as metabolic screening." @default.
• W2923768424 created "2019-04-01" @default.
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• W2923768424 date "2010-03-15" @default.
• W2923768424 modified "2023-09-24" @default.
• W2923768424 title "High-Frequency Dynamic Nuclear Polarization" @default.
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• W2923768424 doi "https://doi.org/10.1002/9780470034590.emrstm1183" @default.
• W2923768424 hasPublicationYear "2010" @default.
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