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- W2923932504 abstract "Polymyxin B and E (colistin), are a group of cationic charged cyclic antibiotic lipopeptides that are frequently used in the clinics to treat infections caused by the multidrug-resistant gram-negative bacteria. Since the interactions with the blood plasma drug-transport proteins may play a critical role in determining their pharmacological and pharmacokinetic profiles, we studied the binding properties of polymyxins to the human serum albumin (HSA) under simulated physiological conditions by the combination of biophysical approaches, such as isothermal titration calorimetry (ITC), fluorescence anisotropy, circular dichroism (CD) buttressed by computational studies. The HSA binding to the polymyxins was relatively strong (Ka ≈ 1.0 × 107 M−1). Molecular docking indicated that polymyxins bind to the cleft of HSA between domains I and III via the electrostatic interactions. This evidence was further confirmed by the entropy-driven interaction for the polymyxins bound HSA. Far UV-CD experiments showed that the secondary structure of HSA doesn't alter and its stable structure is preserved. Collectively, these investigations revealed that the polymyxins bind preferentially to the partially unfolded intermediate forms of the protein structure; however, HSA molecule does not undergo any significant conformational changes upon binding. This is promising as it may limit the unfavorable side effects of the medicine. On the whole, the results provide quantitative and qualitative insight of the binding interaction between HSA and polymyxins, which is important in understanding their effect as therapeutic agents." @default.
- W2923932504 created "2019-04-01" @default.
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- W2923932504 date "2019-06-01" @default.
- W2923932504 modified "2023-09-26" @default.
- W2923932504 title "Polymyxins interaction to the human serum albumin: A thermodynamic and computational study" @default.
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- W2923932504 cites W1960384215 @default.
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- W2923932504 cites W1967459325 @default.
- W2923932504 cites W1968991415 @default.
- W2923932504 cites W1971942980 @default.
- W2923932504 cites W1972758026 @default.
- W2923932504 cites W1974290383 @default.
- W2923932504 cites W1975620431 @default.
- W2923932504 cites W1976499671 @default.
- W2923932504 cites W1984922158 @default.
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- W2923932504 cites W1999902464 @default.
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- W2923932504 cites W2005817231 @default.
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- W2923932504 doi "https://doi.org/10.1016/j.saa.2019.03.077" @default.
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