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- W2924045128 abstract "Abstract Transport of proteins across and into membranes is a fundamental biological process with the vast majority being conducted by the ubiquitous Sec machinery. In bacteria, this is usually achieved when the SecY-complex engages the cytosolic ATPase SecA (secretion) or translating ribosomes (insertion). Great strides have been made towards understanding the mechanism of protein translocation. Yet, important questions remain – notably, the nature of the individual steps that constitute transport, and how the proton-motive force (PMF) across the plasma membrane contributes. Here, we apply a recently developed high-resolution protein transport assay to explore these questions. We find that pre-protein transport is limited primarily by the diffusion of arginine residues across the membrane, particularly in the context of bulky hydrophobic sequences. This specific effect of arginine, caused by its positive charge, is mitigated for lysine which can be deprotonated and transported across the membrane in its neutral form. These observations have interesting implications for the mechanism of protein secretion, suggesting a simple mechanism by which PMF can aid transport, and enabling a ‘proton ratchet’, wherein re-protonation of exiting lysine residues prevents channel re-entry, biasing transport in the outward direction." @default.
- W2924045128 created "2019-04-01" @default.
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- W2924045128 date "2019-03-29" @default.
- W2924045128 modified "2023-09-26" @default.
- W2924045128 title "A ‘proton ratchet’ for coupling the membrane potential to protein transport" @default.
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- W2924045128 doi "https://doi.org/10.1101/592543" @default.
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