FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2924103742> ?p ?o ?g. }

• W2924103742 abstract "Abstract Molecular cloning is an essential technique in molecular biology and biochemistry, but it is frequently laborious when adequate restriction enzyme recognition sites are absent. Cas9 endonucleases can induce site-specific DNA double-strand breaks at sites homologous to their guide RNAs, rendering an alternative to restriction enzymes. Here, by combining DNA cleavage via a Cas9 endonuclease and DNA ligation via Gibson assembly, we demonstrate a precise and practical DNA cloning method for replacing part of a backbone plasmid. We first replaced a resistance marker gene as a proof of concept and next generated DNA plasmids that encode engineered Cas9 variants (VQR, VRER and SpCas9-NG), which target non-canonical NGA, NGCG and NG protospacer-adjacent motif (PAM) sequences, fused with adenosine deaminases for adenine base editing (named VQR-ABE, VRER-ABE and NG-ABE, respectively). Ultimately, we confirmed that the re-constructed plasmids can successfully convert adenosine to guanine at endogenous target sites containing the non-canonical NGA, NGCG and NG PAMs, expanding the targetable range of the adenine base editing." @default.
• W2924103742 created "2019-04-01" @default.
• W2924103742 creator A5025853206 @default.
• W2924103742 creator A5058387827 @default.
• W2924103742 creator A5077099423 @default.
• W2924103742 date "2019-03-20" @default.
• W2924103742 modified "2023-10-16" @default.
• W2924103742 title "Construction of non-canonical PAM-targeting adenosine base editors by restriction enzyme-free DNA cloning using CRISPR-Cas9" @default.
• W2924103742 cites W1213974439 @default.
• W2924103742 cites W1487644672 @default.
• W2924103742 cites W1971439989 @default.
• W2924103742 cites W1984064827 @default.
• W2924103742 cites W1988101725 @default.
• W2924103742 cites W2003911538 @default.
• W2924103742 cites W2096261947 @default.
• W2924103742 cites W2107959600 @default.
• W2924103742 cites W2115603949 @default.
• W2924103742 cites W2122563582 @default.
• W2924103742 cites W2123746851 @default.
• W2924103742 cites W2165869860 @default.
• W2924103742 cites W2169244146 @default.
• W2924103742 cites W2332610736 @default.
• W2924103742 cites W2395741291 @default.
• W2924103742 cites W2509775353 @default.
• W2924103742 cites W2581298520 @default.
• W2924103742 cites W2766599608 @default.
• W2924103742 cites W2804311755 @default.
• W2924103742 cites W2805505209 @default.
• W2924103742 cites W2883029163 @default.
• W2924103742 cites W2883250809 @default.
• W2924103742 cites W2889513238 @default.
• W2924103742 cites W2989794628 @default.
• W2924103742 cites W4245053291 @default.
• W2924103742 cites W4250460376 @default.
• W2924103742 cites W4379389746 @default.
• W2924103742 doi "https://doi.org/10.1038/s41598-019-41356-1" @default.
• W2924103742 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6426851" @default.
• W2924103742 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30894632" @default.
• W2924103742 hasPublicationYear "2019" @default.
• W2924103742 type Work @default.
• W2924103742 sameAs 2924103742 @default.
• W2924103742 citedByCount "27" @default.
• W2924103742 countsByYear W29241037422019 @default.
• W2924103742 countsByYear W29241037422020 @default.
• W2924103742 countsByYear W29241037422021 @default.
• W2924103742 countsByYear W29241037422022 @default.
• W2924103742 countsByYear W29241037422023 @default.
• W2924103742 crossrefType "journal-article" @default.
• W2924103742 hasAuthorship W2924103742A5025853206 @default.
• W2924103742 hasAuthorship W2924103742A5058387827 @default.
• W2924103742 hasAuthorship W2924103742A5077099423 @default.
• W2924103742 hasBestOaLocation W29241037421 @default.
• W2924103742 hasConcept C104317684 @default.
• W2924103742 hasConcept C128319531 @default.
• W2924103742 hasConcept C132455925 @default.
• W2924103742 hasConcept C144501496 @default.
• W2924103742 hasConcept C153911025 @default.
• W2924103742 hasConcept C167625842 @default.
• W2924103742 hasConcept C181199279 @default.
• W2924103742 hasConcept C195116880 @default.
• W2924103742 hasConcept C19924922 @default.
• W2924103742 hasConcept C203284983 @default.
• W2924103742 hasConcept C22744801 @default.
• W2924103742 hasConcept C2776476023 @default.
• W2924103742 hasConcept C2777028655 @default.
• W2924103742 hasConcept C40507961 @default.
• W2924103742 hasConcept C54355233 @default.
• W2924103742 hasConcept C552990157 @default.
• W2924103742 hasConcept C55493867 @default.
• W2924103742 hasConcept C70721500 @default.
• W2924103742 hasConcept C86803240 @default.
• W2924103742 hasConcept C97702854 @default.
• W2924103742 hasConcept C98108389 @default.
• W2924103742 hasConceptScore W2924103742C104317684 @default.
• W2924103742 hasConceptScore W2924103742C128319531 @default.
• W2924103742 hasConceptScore W2924103742C132455925 @default.
• W2924103742 hasConceptScore W2924103742C144501496 @default.
• W2924103742 hasConceptScore W2924103742C153911025 @default.
• W2924103742 hasConceptScore W2924103742C167625842 @default.
• W2924103742 hasConceptScore W2924103742C181199279 @default.
• W2924103742 hasConceptScore W2924103742C195116880 @default.
• W2924103742 hasConceptScore W2924103742C19924922 @default.
• W2924103742 hasConceptScore W2924103742C203284983 @default.
• W2924103742 hasConceptScore W2924103742C22744801 @default.
• W2924103742 hasConceptScore W2924103742C2776476023 @default.
• W2924103742 hasConceptScore W2924103742C2777028655 @default.
• W2924103742 hasConceptScore W2924103742C40507961 @default.
• W2924103742 hasConceptScore W2924103742C54355233 @default.
• W2924103742 hasConceptScore W2924103742C552990157 @default.
• W2924103742 hasConceptScore W2924103742C55493867 @default.
• W2924103742 hasConceptScore W2924103742C70721500 @default.
• W2924103742 hasConceptScore W2924103742C86803240 @default.
• W2924103742 hasConceptScore W2924103742C97702854 @default.
• W2924103742 hasConceptScore W2924103742C98108389 @default.
• W2924103742 hasIssue "1" @default.
• W2924103742 hasLocation W29241037421 @default.
• W2924103742 hasLocation W29241037422 @default.
• W2924103742 hasLocation W29241037423 @default.
• W2924103742 hasLocation W29241037424 @default.
• W2924103742 hasOpenAccess W2924103742 @default.

• next