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- W2924322022 abstract "To minimize undesired pharmacological activities and improve the bioavailability, the fucoidan and fucosylated chondroitin sulfate (FCS) from Holothuria floridana were depolymerized under hydrothermal conditions and the mechanism underlying hydrothermal depolymerization was proposed. Our results demonstrated that fucoidan and FCS from Holothuria floridana were able to be gradually depolymerized without desulfation at 100-121 °C by control of pH at 5-6 to obtain controlled molecular weight. It was the first time to find that pH also plays a key role on the hydrothermal depolymerization of fucoidan and FCS. The monosaccharide composition, FT-IR and NMR analysis showed that the structure of the optimized hydrothermal depolymerized fucoidan and FCS remained almost unchanged. By comparison, FCS was more difficult to be depolymerized than fucoidan under the same hydrothermal condition. The oligosaccharide profile in depolymerized fucoidan and FCS by HILIC-MS analysis further revealed that FCS was depolymerized with preferential cleavage of β-1 → 4 glycosidic linkage and decarboxylation on glucuronic acid during hydrothermal treatment, which was quite different with the random fracture type of fucoidan due to their different structure. These results indicated that hydrothermal depolymerization and action mechanism of fucoidan and FCS from sea cucumber were quite different for their different structure." @default.
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- W2924322022 date "2019-07-01" @default.
- W2924322022 modified "2023-10-16" @default.
- W2924322022 title "Comparison of hydrothermal depolymerization and oligosaccharide profile of fucoidan and fucosylated chondroitin sulfate from Holothuria floridana" @default.
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- W2924322022 doi "https://doi.org/10.1016/j.ijbiomac.2019.03.127" @default.
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