FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2924357846> ?p ?o ?g. }

• W2924357846 endingPage "1343" @default.
• W2924357846 startingPage "1336" @default.
• W2924357846 abstract "Background: It has recently been recognized that serum vimentin is elevated in infectious diseases, and that vimentin plays a role in regulating neutrophils and macrophages associated inflammation. However, the mechanisms are unclear. This study was designed to explore the role of vimentin in regulating monocyte survival or apoptosis as well as inflammatory cytokine secretion in response to lipopolysaccharides (LPSs). Methods: A human monocytic leukemia cell line (THP-1) was transfected with vimentin-specific small interfering RNA (siRNA) or vimentin over-expressing plasmid. Apoptosis was assessed by TdT-mediated dUTP Nick-End Labeling (TUNEL) and DNA content assay. Immunoblotting was performed to detect apoptosis-associated proteins. Cytokines (interleukin [IL]-6, IL-10, and tumor necrosis factor α [TNF-α]) were measured by enzyme-linked immuno sorbent assay. Two-way analysis of variance followed by Student's t test was used to compare means between different groups. Results: Suppression of vimentin in THP-1 cells resulted in increased apoptotic response in the presence of LPS, while over-expression of vimentin could prevent the cells from apoptosis in response to LPS. LPS alone or suppression of vimentin resulted in significant up-regulation of caspase-3 (1.42 ± 0.20 of LPS alone and 1.68 ± 0.10 of vimentin suppression vs. control, t = 5.21 and 10.28, respectively, P < 0.05). In addition, pro-inflammatory cytokines (IL-6 and TNF-α) was significantly increased (IL-6: 577.90 ± 159.90 pg/day/105 cells vs. 283.80 ± 124.60 pg/day/105 cells of control, t = 14.76, P < 0.05; TNF-α: 54.10 ± 5.80 vs. 17.10 ± 0.10 pg/day/105 cells of control, t = 6.71, P < 0.05), while anti-inflammatory cytokine (IL-10) was significantly up-regulated in the THP-1 cells that over-expressed vimentin (140.9 ± 17.2 pg/day/105 cells vs. undetectable in control cells). Conclusions: In summary, the vimentin may regulate innate immunity through modulating monocytes viability as well as inflammatory response in sepsis through shifting the balance of pro-inflammatory and anti-inflammatory cytokines." @default.
• W2924357846 created "2019-04-01" @default.
• W2924357846 creator A5001884955 @default.
• W2924357846 creator A5004491347 @default.
• W2924357846 creator A5018735035 @default.
• W2924357846 creator A5033153999 @default.
• W2924357846 creator A5051747497 @default.
• W2924357846 creator A5084906179 @default.
• W2924357846 date "2019-06-05" @default.
• W2924357846 modified "2023-10-16" @default.
• W2924357846 title "Vimentin modulates apoptosis and inflammatory cytokine release by a human monocytic cell line (THP-1) in response to lipopolysaccharides in vitro" @default.
• W2924357846 cites W1657172089 @default.
• W2924357846 cites W1968856363 @default.
• W2924357846 cites W1985860758 @default.
• W2924357846 cites W1996340200 @default.
• W2924357846 cites W1999752997 @default.
• W2924357846 cites W2007070822 @default.
• W2924357846 cites W2021816178 @default.
• W2924357846 cites W2022448990 @default.
• W2924357846 cites W2033787709 @default.
• W2924357846 cites W2047809394 @default.
• W2924357846 cites W2083381184 @default.
• W2924357846 cites W2083513831 @default.
• W2924357846 cites W2093977796 @default.
• W2924357846 cites W2127910803 @default.
• W2924357846 cites W2131420737 @default.
• W2924357846 cites W2139547086 @default.
• W2924357846 cites W2143325804 @default.
• W2924357846 cites W2147406541 @default.
• W2924357846 cites W2164164305 @default.
• W2924357846 cites W2300217793 @default.
• W2924357846 cites W2337992351 @default.
• W2924357846 cites W2509596317 @default.
• W2924357846 cites W2521799152 @default.
• W2924357846 cites W2538997962 @default.
• W2924357846 doi "https://doi.org/10.1097/cm9.0000000000000187" @default.
• W2924357846 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6629360" @default.
• W2924357846 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30882463" @default.
• W2924357846 hasPublicationYear "2019" @default.
• W2924357846 type Work @default.
• W2924357846 sameAs 2924357846 @default.
• W2924357846 citedByCount "7" @default.
• W2924357846 countsByYear W29243578462021 @default.
• W2924357846 countsByYear W29243578462022 @default.
• W2924357846 countsByYear W29243578462023 @default.
• W2924357846 crossrefType "journal-article" @default.
• W2924357846 hasAuthorship W2924357846A5001884955 @default.
• W2924357846 hasAuthorship W2924357846A5004491347 @default.
• W2924357846 hasAuthorship W2924357846A5018735035 @default.
• W2924357846 hasAuthorship W2924357846A5033153999 @default.
• W2924357846 hasAuthorship W2924357846A5051747497 @default.
• W2924357846 hasAuthorship W2924357846A5084906179 @default.
• W2924357846 hasBestOaLocation W29243578461 @default.
• W2924357846 hasConcept C147447768 @default.
• W2924357846 hasConcept C153911025 @default.
• W2924357846 hasConcept C17991360 @default.
• W2924357846 hasConcept C190283241 @default.
• W2924357846 hasConcept C196795494 @default.
• W2924357846 hasConcept C203014093 @default.
• W2924357846 hasConcept C204232928 @default.
• W2924357846 hasConcept C204258353 @default.
• W2924357846 hasConcept C2778690821 @default.
• W2924357846 hasConcept C2780943621 @default.
• W2924357846 hasConcept C2781184567 @default.
• W2924357846 hasConcept C502942594 @default.
• W2924357846 hasConcept C54355233 @default.
• W2924357846 hasConcept C55493867 @default.
• W2924357846 hasConcept C81885089 @default.
• W2924357846 hasConcept C86803240 @default.
• W2924357846 hasConceptScore W2924357846C147447768 @default.
• W2924357846 hasConceptScore W2924357846C153911025 @default.
• W2924357846 hasConceptScore W2924357846C17991360 @default.
• W2924357846 hasConceptScore W2924357846C190283241 @default.
• W2924357846 hasConceptScore W2924357846C196795494 @default.
• W2924357846 hasConceptScore W2924357846C203014093 @default.
• W2924357846 hasConceptScore W2924357846C204232928 @default.
• W2924357846 hasConceptScore W2924357846C204258353 @default.
• W2924357846 hasConceptScore W2924357846C2778690821 @default.
• W2924357846 hasConceptScore W2924357846C2780943621 @default.
• W2924357846 hasConceptScore W2924357846C2781184567 @default.
• W2924357846 hasConceptScore W2924357846C502942594 @default.
• W2924357846 hasConceptScore W2924357846C54355233 @default.
• W2924357846 hasConceptScore W2924357846C55493867 @default.
• W2924357846 hasConceptScore W2924357846C81885089 @default.
• W2924357846 hasConceptScore W2924357846C86803240 @default.
• W2924357846 hasIssue "11" @default.
• W2924357846 hasLocation W29243578461 @default.
• W2924357846 hasLocation W29243578462 @default.
• W2924357846 hasLocation W29243578463 @default.
• W2924357846 hasLocation W29243578464 @default.
• W2924357846 hasLocation W29243578465 @default.
• W2924357846 hasOpenAccess W2924357846 @default.
• W2924357846 hasPrimaryLocation W29243578461 @default.
• W2924357846 hasRelatedWork W2008987678 @default.
• W2924357846 hasRelatedWork W2017946641 @default.
• W2924357846 hasRelatedWork W2028288720 @default.
• W2924357846 hasRelatedWork W2050503072 @default.
• W2924357846 hasRelatedWork W2053270887 @default.

• next