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• W2925263694 abstract "Cyclic nucleotide–gated (CNG) channels produce the initial electrical signal in mammalian vision and olfaction. They open in response to direct binding of cyclic nucleotide (cAMP or cGMP) to a cytoplasmic region of the channel. However, the conformational rearrangements occurring upon binding to produce pore opening (i.e. gating) are not well understood. SthK is a bacterial CNG channel that has the potential to serve as an ideal model for structure–function studies of gating but is currently limited by its toxicity, native cysteines, and low open probability (Po). Here, we expressed SthK in giant Escherichia coli spheroplasts and performed patch-clamp recordings to characterize SthK gating in a bacterial membrane. We demonstrated that the Po in cAMP is higher than has been previously published and that cGMP acts as a weak partial SthK agonist. Additionally, we determined that SthK expression is toxic to E. coli because of gating by cytoplasmic cAMP. We overcame this toxicity by developing an adenylate cyclase–knockout E. coli cell line. Finally, we generated a cysteine-free SthK construct and introduced mutations that further increase the Po in cAMP. We propose that this SthK model will help elucidate the gating mechanism of CNG channels." @default.
• W2925263694 created "2019-04-01" @default.
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• W2925263694 date "2019-05-01" @default.
• W2925263694 modified "2023-10-15" @default.
• W2925263694 title "Functional characterization and optimization of a bacterial cyclic nucleotide–gated channel" @default.
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• W2925263694 doi "https://doi.org/10.1074/jbc.ra119.007699" @default.
• W2925263694 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6509488" @default.
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• W2925263694 hasPublicationYear "2019" @default.
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