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- W292583603 abstract "[Objective]The aim was to provide a theoretical basis for preparation of polyclonal antibodies and monoclonal antibody that of type specificity,as well as Foot-and-mouth Disease Virus(FMDV) typing.[Method] The recombinant plasmid pGEM-CP1 that contained VP1 gene of FMDV of serotype C was used as template for the VP1 and its C terminus coding fragments of FMDV of serotypes C amplification.The coding fragments of VP1 and its C terminus were respectively cloned into prokaryotic expression vector for prokaryotic expression and the reactionogenicity was detected.The purified fusion protein of FMDV VP1 and its C terminus of serotype C were used to construct the indirect ELISA method to detect positive sera of four serotypes A,O,C and Asia 1 of guinea pig and determine the cross reactivity of FMDV antibody of VP1 and its C terminus of serotype C with other three serotypes.[Result]The recombinant prokaryotic expression plasmids of pPRO-CVP1 and pPRO-CVP1c were constructed,FMDV VP1 and its C terminus of serotype C were expressed in high level,and the molecular weight of target proteins was 33 kD and 20 kD respectively.Western blot result showed that the fusion protein of VP1 and its C terminus could react with the positive sera of guinea pig of the same serotype.ELISA results revealed that VP1 and its C terminus of serotype C are type-specific and no cross-reactivities were shown between guinea pig positive sera of FMDV of serotype C with the other three serotypes,and the C terminus showed better type-specificity.[Conclusion] FMDV specific antigen of serotype C was obtained." @default.
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- W292583603 date "2009-01-01" @default.
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- W292583603 title "Expression and Identification of the Type-specific Antigen of FMDV of Serotype C" @default.
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