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• W2926661337 endingPage "1683" @default.
• W2926661337 startingPage "1683" @default.
• W2926661337 abstract "Stearidonic acid (SDA; 18:4, n-3) is the delta 15-desaturase product of gamma linolenic acid (GLA; 18:3, n-6) and delta 6-desaturase product of alpha linolenic acid (ALA; 18:3, n-3). Construction of engineered oleaginous microbes have been attracting significant interest in producing SDA because of its nutritional value and pharmaceutical applications. Mucor circinelloides is a GLA producing filamentous fungus, which can be a useful tool to produce SDA. This study has, therefore, overexpressed the delta-15 desaturase (D15D) gene from Mortierella alpina in this fungus to construct a SDA-producing cell factory. To produce SDA in M. circinelloides, the homologous overexpression of D15D gene was analyzed. When the gene was overexpressed in M. circinelloides CBS 277.49, up to 5.0% SDA was accumulated in this strain. According to current knowledge, this is the first study describing the construction of a SDA-producing cell factory by overexpression of D15D gene in oleaginous fungus M. circinelloides. A new scope for further research has been established by this work to improve SDA production in this fungus, specifically in its high lipid-producing strain, WJ11." @default.
• W2926661337 created "2019-04-11" @default.
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• W2926661337 date "2019-04-04" @default.
• W2926661337 modified "2023-10-02" @default.
• W2926661337 title "Genetic Modification of Mucor circinelloides to Construct Stearidonic Acid Producing Cell Factory" @default.
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• W2926661337 doi "https://doi.org/10.3390/ijms20071683" @default.
• W2926661337 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6480972" @default.
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• W2926661337 hasPublicationYear "2019" @default.
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