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• W2927190089 endingPage "018" @default.
• W2927190089 startingPage "003" @default.
• W2927190089 abstract "The polymerase chain reaction (PCR) is a primer-mediated enzymatic amplification of specifically cloned or genomic DNA sequences (). This PCR process, invented more than a decade ago, has been automated for routine use in laboratories worldwide. The template DNA contains the target sequence, which may be tens or tens of thousands of nucleotides in length. A thermostable DNA polymerase such as Taq DNA polymerse, catalyzes the buffered reaction in which an excess of an oligonucleotide primer pair and four deoxynucleoside triphosphates (dNTPs) are used to make millions of copies of the target sequence. Although the purpose of the PCR process is to amplify template DNA, a reverse transcription step allows the starting point to be RNA (-)." @default.
• W2927190089 created "2019-04-11" @default.
• W2927190089 creator A5021871771 @default.
• W2927190089 creator A5027464237 @default.
• W2927190089 date "2003-11-14" @default.
• W2927190089 modified "2023-10-17" @default.
• W2927190089 title "Polymerase Chain Reaction: Basic Principles and Routine Practice" @default.
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• W2927190089 doi "https://doi.org/10.1385/1-59259-177-9:003" @default.
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