Matches in SemOpenAlex for { <https://semopenalex.org/work/W2927195280> ?p ?o ?g. }
- W2927195280 endingPage "5230" @default.
- W2927195280 startingPage "5223" @default.
- W2927195280 abstract "Microbes have the unique ability to acquire immunological memories from mobile genetic invaders to protect themselves from predation. To confer CRISPR resistance, new spacers need to be compatible with a targeting requirement in the invader's DNA called the protospacer adjacent motif (PAM). Many CRISPR systems encode Cas4 proteins to ensure new spacers are integrated that meet this targeting prerequisite. Here we report that a gene fusion between cas4 and cas1 from the Geobacter sulfurreducens I-U CRISPR-Cas system is capable of introducing functional spacers carrying interference proficient TTN PAM sequences at much higher frequencies than unfused Cas4 adaptation modules. Mutations of Cas4-domain catalytic residues resulted in dramatically decreased naïve and primed spacer acquisition, and a loss of PAM selectivity showing that the Cas4 domain controls Cas1 activity. We propose the fusion gene evolved to drive the acquisition of only PAM-compatible spacers to optimize CRISPR interference." @default.
- W2927195280 created "2019-04-11" @default.
- W2927195280 creator A5005439170 @default.
- W2927195280 creator A5012680193 @default.
- W2927195280 creator A5051219873 @default.
- W2927195280 creator A5084626714 @default.
- W2927195280 date "2019-04-02" @default.
- W2927195280 modified "2023-10-18" @default.
- W2927195280 title "Cas4–Cas1 fusions drive efficient PAM selection and control CRISPR adaptation" @default.
- W2927195280 cites W1967046461 @default.
- W2927195280 cites W1990487336 @default.
- W2927195280 cites W2000277549 @default.
- W2927195280 cites W2004317124 @default.
- W2927195280 cites W2006494972 @default.
- W2927195280 cites W2010986636 @default.
- W2927195280 cites W2017721099 @default.
- W2927195280 cites W2035803293 @default.
- W2927195280 cites W2046563455 @default.
- W2927195280 cites W2077190448 @default.
- W2927195280 cites W2132440004 @default.
- W2927195280 cites W2142516938 @default.
- W2927195280 cites W2149594762 @default.
- W2927195280 cites W2149751339 @default.
- W2927195280 cites W2164807693 @default.
- W2927195280 cites W2169244146 @default.
- W2927195280 cites W2199695484 @default.
- W2927195280 cites W2271162534 @default.
- W2927195280 cites W2288272959 @default.
- W2927195280 cites W2465903376 @default.
- W2927195280 cites W2513552598 @default.
- W2927195280 cites W2566705644 @default.
- W2927195280 cites W2604453086 @default.
- W2927195280 cites W2608395333 @default.
- W2927195280 cites W2623277795 @default.
- W2927195280 cites W2626530223 @default.
- W2927195280 cites W2738807597 @default.
- W2927195280 cites W2770083313 @default.
- W2927195280 cites W2773261850 @default.
- W2927195280 cites W2795218006 @default.
- W2927195280 cites W2795314419 @default.
- W2927195280 cites W2806533962 @default.
- W2927195280 cites W2808563997 @default.
- W2927195280 cites W2887757117 @default.
- W2927195280 cites W2889006480 @default.
- W2927195280 cites W2889457199 @default.
- W2927195280 cites W2902503611 @default.
- W2927195280 cites W2912107519 @default.
- W2927195280 cites W2951849777 @default.
- W2927195280 cites W2952862039 @default.
- W2927195280 doi "https://doi.org/10.1093/nar/gkz217" @default.
- W2927195280 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6547450" @default.
- W2927195280 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30937444" @default.
- W2927195280 hasPublicationYear "2019" @default.
- W2927195280 type Work @default.
- W2927195280 sameAs 2927195280 @default.
- W2927195280 citedByCount "32" @default.
- W2927195280 countsByYear W29271952802019 @default.
- W2927195280 countsByYear W29271952802020 @default.
- W2927195280 countsByYear W29271952802021 @default.
- W2927195280 countsByYear W29271952802022 @default.
- W2927195280 countsByYear W29271952802023 @default.
- W2927195280 crossrefType "journal-article" @default.
- W2927195280 hasAuthorship W2927195280A5005439170 @default.
- W2927195280 hasAuthorship W2927195280A5012680193 @default.
- W2927195280 hasAuthorship W2927195280A5051219873 @default.
- W2927195280 hasAuthorship W2927195280A5084626714 @default.
- W2927195280 hasBestOaLocation W29271952801 @default.
- W2927195280 hasConcept C104317684 @default.
- W2927195280 hasConcept C144501496 @default.
- W2927195280 hasConcept C154055011 @default.
- W2927195280 hasConcept C54355233 @default.
- W2927195280 hasConcept C552990157 @default.
- W2927195280 hasConcept C70721500 @default.
- W2927195280 hasConcept C86803240 @default.
- W2927195280 hasConcept C98108389 @default.
- W2927195280 hasConceptScore W2927195280C104317684 @default.
- W2927195280 hasConceptScore W2927195280C144501496 @default.
- W2927195280 hasConceptScore W2927195280C154055011 @default.
- W2927195280 hasConceptScore W2927195280C54355233 @default.
- W2927195280 hasConceptScore W2927195280C552990157 @default.
- W2927195280 hasConceptScore W2927195280C70721500 @default.
- W2927195280 hasConceptScore W2927195280C86803240 @default.
- W2927195280 hasConceptScore W2927195280C98108389 @default.
- W2927195280 hasFunder F4320334678 @default.
- W2927195280 hasIssue "10" @default.
- W2927195280 hasLocation W29271952801 @default.
- W2927195280 hasLocation W29271952802 @default.
- W2927195280 hasLocation W29271952803 @default.
- W2927195280 hasLocation W29271952804 @default.
- W2927195280 hasLocation W29271952805 @default.
- W2927195280 hasOpenAccess W2927195280 @default.
- W2927195280 hasPrimaryLocation W29271952801 @default.
- W2927195280 hasRelatedWork W2344454439 @default.
- W2927195280 hasRelatedWork W2765809583 @default.
- W2927195280 hasRelatedWork W2890863912 @default.
- W2927195280 hasRelatedWork W2893434308 @default.
- W2927195280 hasRelatedWork W3041319315 @default.
- W2927195280 hasRelatedWork W3042012321 @default.