FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2927309498> ?p ?o ?g. }

• W2927309498 endingPage "1740" @default.
• W2927309498 startingPage "1740" @default.
• W2927309498 abstract "Beet cyst nematode (Heterodera schachtii Schm.) is one of the most damaging pests in sugar beet growing areas around the world. The Hs1pro-1 and cZR3 genes confer resistance to the beet cyst nematode, and both were cloned from sugar beet translocation line (A906001). The translocation line carried the locus from B. procumbens chromosome 1 including Hs1pro-1 gene and resistance gene analogs (RGA), which confer resistance to Heterodera schachtii. In this research, BvHs1pro-1 and BvcZR3 genes were transferred into oilseed rape to obtain different transgenic lines by A. tumefaciens mediated transformation method. The cZR3Hs1pro-1 gene was pyramided into the same plants by crossing homozygous cZR3 and Hs1pro-1 plants to identify the function and interaction of cZR3 and Hs1pro-1 genes. In vitro and in vivo cyst nematode resistance tests showed that cZR3 and Hs1pro-1 plants could be infested by beet cyst nematode (BCN) juveniles, however a large fraction of penetrated nematode juveniles was not able to develop normally and stagnated in roots of transgenic plants, consequently resulting in a significant reduction in the number of developed nematode females. A higher efficiency in inhibition of nematode females was observed in plants expressing pyramiding genes than in those only expressing a single gene. Molecular analysis demonstrated that BvHs1pro-1 and BvcZR3 gene expressions in oilseed rape constitutively activated transcription of plant-defense related genes such as NPR1 (non-expresser of PR1), SGT1b (enhanced disease resistance 1) and RAR1 (suppressor of the G2 allele of skp1). Transcript of NPR1 gene in transgenic cZR3 and Hs1pro-1 plants were slightly up-regulated, while its expression was considerably enhanced in cZR3Hs1pro-1 gene pyramiding plants. The expression of EDS1 gene did not change significantly among transgenic cZR3, Hs1pro-1 and cZR3Hs1pro-1 gene pyramiding plants and wild type. The expression of SGT1b gene was slightly up-regulated in transgenic cZR3 and Hs1pro-1 plants compared with the wild type, however, its expression was not changed in cZR3Hs1pro-1 gene pyramiding plant and had no interaction effect. RAR1 gene expression was significantly up-regulated in transgenic cZR3 and cZR3Hs1pro-1 genes pyramiding plants, but almost no expression was found in Hs1pro-1 transgenic plants. These results show that nematode resistance genes from sugar beet were functional in oilseed rape and conferred BCN resistance by activation of a CC-NBS-LRR R gene mediated resistance response. The gene pyramiding had enhanced resistance, thus offering a novel approach for the BCN control by preventing the propagation of BCN in oilseed rape. The transgenic oilseed rape could be used as a trap crop to offer an alternative method for beet cyst nematode control." @default.
• W2927309498 created "2019-04-11" @default.
• W2927309498 creator A5009934339 @default.
• W2927309498 creator A5012860349 @default.
• W2927309498 creator A5016723726 @default.
• W2927309498 creator A5023367635 @default.
• W2927309498 creator A5089824358 @default.
• W2927309498 date "2019-04-08" @default.
• W2927309498 modified "2023-10-14" @default.
• W2927309498 title "BvcZR3 and BvHs1pro-1 Genes Pyramiding Enhanced Beet Cyst Nematode (Heterodera schachtii Schm.) Resistance in Oilseed Rape (Brassica napus L.)" @default.
• W2927309498 cites W1513838670 @default.
• W2927309498 cites W1901378084 @default.
• W2927309498 cites W1973328229 @default.
• W2927309498 cites W1975489017 @default.
• W2927309498 cites W1976365858 @default.
• W2927309498 cites W1977537183 @default.
• W2927309498 cites W1986587865 @default.
• W2927309498 cites W1988006507 @default.
• W2927309498 cites W1993215296 @default.
• W2927309498 cites W1993320561 @default.
• W2927309498 cites W1993953131 @default.
• W2927309498 cites W1995968862 @default.
• W2927309498 cites W2002459418 @default.
• W2927309498 cites W2003912358 @default.
• W2927309498 cites W2007723705 @default.
• W2927309498 cites W2009631310 @default.
• W2927309498 cites W2011362687 @default.
• W2927309498 cites W2014870946 @default.
• W2927309498 cites W2021106676 @default.
• W2927309498 cites W2024556021 @default.
• W2927309498 cites W2030375090 @default.
• W2927309498 cites W2047646893 @default.
• W2927309498 cites W2050415577 @default.
• W2927309498 cites W2052136236 @default.
• W2927309498 cites W2052444990 @default.
• W2927309498 cites W2059839957 @default.
• W2927309498 cites W2066900966 @default.
• W2927309498 cites W2077545872 @default.
• W2927309498 cites W2082007927 @default.
• W2927309498 cites W2082044466 @default.
• W2927309498 cites W2091123240 @default.
• W2927309498 cites W2098188021 @default.
• W2927309498 cites W2098749551 @default.
• W2927309498 cites W2099211368 @default.
• W2927309498 cites W2103508580 @default.
• W2927309498 cites W2107277218 @default.
• W2927309498 cites W2114822584 @default.
• W2927309498 cites W2116672493 @default.
• W2927309498 cites W2121711582 @default.
• W2927309498 cites W2122246965 @default.
• W2927309498 cites W2129438438 @default.
• W2927309498 cites W2130029642 @default.
• W2927309498 cites W2134415383 @default.
• W2927309498 cites W2138168142 @default.
• W2927309498 cites W2151164890 @default.
• W2927309498 cites W2156301059 @default.
• W2927309498 cites W2156988845 @default.
• W2927309498 cites W2169898159 @default.
• W2927309498 cites W2176810869 @default.
• W2927309498 cites W2192080449 @default.
• W2927309498 cites W25926475 @default.
• W2927309498 cites W2730836514 @default.
• W2927309498 cites W596373422 @default.
• W2927309498 doi "https://doi.org/10.3390/ijms20071740" @default.
• W2927309498 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6479909" @default.
• W2927309498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30965683" @default.
• W2927309498 hasPublicationYear "2019" @default.
• W2927309498 type Work @default.
• W2927309498 sameAs 2927309498 @default.
• W2927309498 citedByCount "20" @default.
• W2927309498 countsByYear W29273094982019 @default.
• W2927309498 countsByYear W29273094982020 @default.
• W2927309498 countsByYear W29273094982021 @default.
• W2927309498 countsByYear W29273094982022 @default.
• W2927309498 countsByYear W29273094982023 @default.
• W2927309498 crossrefType "journal-article" @default.
• W2927309498 hasAuthorship W2927309498A5009934339 @default.
• W2927309498 hasAuthorship W2927309498A5012860349 @default.
• W2927309498 hasAuthorship W2927309498A5016723726 @default.
• W2927309498 hasAuthorship W2927309498A5023367635 @default.
• W2927309498 hasAuthorship W2927309498A5089824358 @default.
• W2927309498 hasBestOaLocation W29273094981 @default.
• W2927309498 hasConcept C102230213 @default.
• W2927309498 hasConcept C104317684 @default.
• W2927309498 hasConcept C122172344 @default.
• W2927309498 hasConcept C144027150 @default.
• W2927309498 hasConcept C18903297 @default.
• W2927309498 hasConcept C2776936025 @default.
• W2927309498 hasConcept C2778364763 @default.
• W2927309498 hasConcept C2778830712 @default.
• W2927309498 hasConcept C2778930020 @default.
• W2927309498 hasConcept C2779553229 @default.
• W2927309498 hasConcept C2780079514 @default.
• W2927309498 hasConcept C54355233 @default.
• W2927309498 hasConcept C59822182 @default.
• W2927309498 hasConcept C86803240 @default.
• W2927309498 hasConcept C93678976 @default.
• W2927309498 hasConceptScore W2927309498C102230213 @default.

• next