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• W2927651564 endingPage "3656" @default.
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• W2927651564 abstract "MicroRNAs (miRNAs), key regulators of gene expression at the post-transcriptional level, are grossly misregulated in some human cancers, including non-small-cell lung carcinoma (NSCLC). The aberrant expression of specific miRNAs results in the abnormal regulation of key components of signalling pathways in tumour cells. MiRNA levels and the activity of the gene targets, including oncogenes and tumour suppressors, produce feedback that changes miRNA expression levels and indicates the cell’s genetic activity. In this study, we measured the expression of five circulating miRNAs (miR-195, miR-504, miR-122, miR-10b and miR-21) and evaluated their association with EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR) mutation status in 66 NSCLC patients. Moreover, we examined the discriminative power of circulating miRNAs for EGFR mutant‐positive and -negative NSCLC patients using two different data normalisation approaches. We extracted total RNA from the plasma of 66 non-squamous NSCLC patients (31 of whom had tumours with EGFR mutations) and measured circulating miRNA levels using quantitative reverse transcription polymerase chain reaction (RT-qPCR). The miRNA expression levels were normalised using two endogenous controls: miR-191 and miR-16. We found significant associations between the expression of circulating miR-504 and EGFR-activating mutations in NSCLC patients regardless of the normalisation approach used (p = 0.0072 and 0.0236 for miR-16 and miR-191 normalisation, respectively). The greatest discriminative power of circulating miR-504 was observed in patients with EGFR exon 19 deletions versus wild-type EGFR normalised to miR-191 (area under the curve (AUC) = 0.81, p < 0.0001). Interestingly, circulating miR-504 levels were significantly reduced in the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS)-mutated subgroup compared to EGFR-mutated patients (p < 0.0030) and those with EGFR/KRAS wild-type tumours (p < 0.0359). Our study demonstrated the feasibility and potential diagnostic value of plasma miR-504 expression analysis to distinguish between EGFR-mutated and wild-type NSCLC patients. However, quality control and normalisation strategies are very important and have a major impact on the outcomes of circulating miRNA analyses." @default.
• W2927651564 created "2019-04-11" @default.
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• W2927651564 date "2019-04-05" @default.
• W2927651564 modified "2023-10-07" @default.
• W2927651564 title "The expression of circulating miR-504 in plasma is associated with EGFR mutation status in non-small-cell lung carcinoma patients" @default.
• W2927651564 cites W1488660005 @default.
• W2927651564 cites W1489886027 @default.
• W2927651564 cites W1577577364 @default.
• W2927651564 cites W1953677527 @default.
• W2927651564 cites W1959917743 @default.
• W2927651564 cites W1968114652 @default.
• W2927651564 cites W1971112547 @default.
• W2927651564 cites W1988163167 @default.
• W2927651564 cites W1995320913 @default.
• W2927651564 cites W2005109475 @default.
• W2927651564 cites W2007118438 @default.
• W2927651564 cites W2012913468 @default.
• W2927651564 cites W2019812252 @default.
• W2927651564 cites W2023816433 @default.
• W2927651564 cites W2024063260 @default.
• W2927651564 cites W2026338982 @default.
• W2927651564 cites W2027735722 @default.
• W2927651564 cites W2048538776 @default.
• W2927651564 cites W2053003053 @default.
• W2927651564 cites W2053551121 @default.
• W2927651564 cites W2060711122 @default.
• W2927651564 cites W2065920909 @default.
• W2927651564 cites W2080092755 @default.
• W2927651564 cites W2080671488 @default.
• W2927651564 cites W2091656909 @default.
• W2927651564 cites W2092913756 @default.
• W2927651564 cites W2099824652 @default.
• W2927651564 cites W2104806799 @default.
• W2927651564 cites W2104960492 @default.
• W2927651564 cites W2105316240 @default.
• W2927651564 cites W2105423509 @default.
• W2927651564 cites W2107277218 @default.
• W2927651564 cites W2111927373 @default.
• W2927651564 cites W2115277036 @default.
• W2927651564 cites W2122553809 @default.
• W2927651564 cites W2124268899 @default.
• W2927651564 cites W2125486087 @default.
• W2927651564 cites W2151868278 @default.
• W2927651564 cites W2153780208 @default.
• W2927651564 cites W2157793936 @default.
• W2927651564 cites W2166084034 @default.
• W2927651564 cites W2166875868 @default.
• W2927651564 cites W2168348685 @default.
• W2927651564 cites W2192080449 @default.
• W2927651564 cites W2210138065 @default.
• W2927651564 cites W2258579332 @default.
• W2927651564 cites W2329284243 @default.
• W2927651564 cites W2334927984 @default.
• W2927651564 cites W2347048968 @default.
• W2927651564 cites W2399866110 @default.
• W2927651564 cites W2432456519 @default.
• W2927651564 cites W2442941134 @default.
• W2927651564 cites W2560568273 @default.
• W2927651564 cites W2565077351 @default.
• W2927651564 cites W2567615400 @default.
• W2927651564 cites W2584153090 @default.
• W2927651564 cites W2589289345 @default.
• W2927651564 cites W2595960505 @default.
• W2927651564 cites W2600355778 @default.
• W2927651564 cites W2604580902 @default.
• W2927651564 cites W2609001553 @default.
• W2927651564 cites W2612797603 @default.
• W2927651564 cites W2743605523 @default.
• W2927651564 cites W2760536705 @default.
• W2927651564 cites W2762724643 @default.
• W2927651564 cites W2767129858 @default.
• W2927651564 cites W2767917697 @default.
• W2927651564 cites W2771897573 @default.
• W2927651564 cites W2784538417 @default.
• W2927651564 cites W2790968539 @default.
• W2927651564 cites W2794844459 @default.
• W2927651564 cites W2795913084 @default.
• W2927651564 cites W2796871386 @default.
• W2927651564 cites W2807519281 @default.
• W2927651564 cites W2883160489 @default.
• W2927651564 cites W2888428272 @default.
• W2927651564 doi "https://doi.org/10.1007/s00018-019-03089-2" @default.
• W2927651564 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6697756" @default.

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