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• W2927697098 startingPage "7395" @default.
• W2927697098 abstract "Abstract Despite growing research efforts on the preparation of (bio)functional liposomes, synthetic capsules cannot reach the densities of protein loading and the control over peptide display that is achieved by natural vesicles. Herein, a microbial platform for high‐yield production of lipidic nanovesicles with clickable thiol moieties in their outer corona is reported. These nanovesicles show low size dispersity, are decorated with a dense, perfectly oriented, and customizable corona of transmembrane polypeptides. Furthermore, this approach enables encapsulation of soluble proteins into the nanovesicles. Due to the mild preparation and loading conditions (absence of organic solvents, pH gradients, or detergents) and their straightforward surface functionalization, which takes advantage of the diversity of commercially available maleimide derivatives, bacteria‐based proteoliposomes are an attractive eco‐friendly alternative that can outperform currently used liposomes." @default.
• W2927697098 created "2019-04-11" @default.
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• W2927697098 date "2019-04-23" @default.
• W2927697098 modified "2023-10-18" @default.
• W2927697098 title "Bacteria‐Based Production of Thiol‐Clickable, Genetically Encoded Lipid Nanovesicles" @default.
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• W2927697098 doi "https://doi.org/10.1002/anie.201902929" @default.
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• W2927697098 hasPublicationYear "2019" @default.
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