Matches in SemOpenAlex for { <https://semopenalex.org/work/W2927763954> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2927763954 abstract "Intoxication by Moraea pallida Bak. (yellow tulp) in livestock is of great importance in South Africa, ranking top among all plant-induced cardiac glycoside toxicosis. The toxic principle, a bufadienolide, is 1α, 2α-epoxyscillirosidine. Treatment of poisoning is challenging and affected livestock often succumbs due to the stress of handling. Manipulating animals to resist poisoning is a potential management strategy. The goal of this study was to explore the potential to develop a vaccine against epoxyscillirosidine by raising antibodies against epoxyscillirosidine in sheep and to assess the neutralization ability of the antibodies in vitro. Epoxyscillirosidine was successfully conjugated to keyhole limpet haemocyanin (KLH) and bovine serum albumin (BSA) rendering them immunogenic. The sheep, vaccinated with epoxyscillirosidine-KLH conjugate (n = 4) and KLH (n = 2) with Montanide, developed antibodies as determined with an indirect enzyme linked immunosorbent assay (ELISA). Total immunoglobulins from sera of vaccinated and control sheep that were purified and concentrated using ammonium sulphate precipitation were 11,940 and 7850 μg, respectively. The in vitro neutralization assay using the methyl blue tetrazolium bromide (MTT) cell viability assay indicated no significant difference (p > 0.05) between anti-epoxyscillirosidine-KLH and KLH antibodies. Rather, the antibodies seemed to enhance the cytotoxicity of epoxyscillirosidine in H9c2 cells. Thus, it is necessary to develop improved vaccination methods to generate antibodies capable of neutralizing the functional group responsible for epoxyscillirosidine toxicity." @default.
- W2927763954 created "2019-04-11" @default.
- W2927763954 creator A5018020143 @default.
- W2927763954 creator A5020462682 @default.
- W2927763954 creator A5025524753 @default.
- W2927763954 creator A5042861404 @default.
- W2927763954 date "2019-06-01" @default.
- W2927763954 modified "2023-09-23" @default.
- W2927763954 title "Evaluation of in vitro neutralization of epoxyscillirosidine by antibodies raised in sheep" @default.
- W2927763954 cites W1419469134 @default.
- W2927763954 cites W143852957 @default.
- W2927763954 cites W1558286913 @default.
- W2927763954 cites W1588888190 @default.
- W2927763954 cites W1971022434 @default.
- W2927763954 cites W1978788492 @default.
- W2927763954 cites W2005206971 @default.
- W2927763954 cites W2013543590 @default.
- W2927763954 cites W2019288421 @default.
- W2927763954 cites W2025182509 @default.
- W2927763954 cites W2038221909 @default.
- W2927763954 cites W2047890402 @default.
- W2927763954 cites W2052888628 @default.
- W2927763954 cites W2057765236 @default.
- W2927763954 cites W2092749220 @default.
- W2927763954 cites W2104793701 @default.
- W2927763954 cites W2152813733 @default.
- W2927763954 cites W2164742140 @default.
- W2927763954 cites W2169468872 @default.
- W2927763954 cites W2170615508 @default.
- W2927763954 cites W2178824608 @default.
- W2927763954 cites W2186681377 @default.
- W2927763954 cites W2320423972 @default.
- W2927763954 cites W2327040567 @default.
- W2927763954 cites W2340617728 @default.
- W2927763954 cites W2401735285 @default.
- W2927763954 cites W2408896847 @default.
- W2927763954 cites W2461020837 @default.
- W2927763954 cites W2757576695 @default.
- W2927763954 cites W336478214 @default.
- W2927763954 cites W69415896 @default.
- W2927763954 doi "https://doi.org/10.1016/j.toxicon.2019.03.023" @default.
- W2927763954 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30946914" @default.
- W2927763954 hasPublicationYear "2019" @default.
- W2927763954 type Work @default.
- W2927763954 sameAs 2927763954 @default.
- W2927763954 citedByCount "1" @default.
- W2927763954 countsByYear W29277639542019 @default.
- W2927763954 crossrefType "journal-article" @default.
- W2927763954 hasAuthorship W2927763954A5018020143 @default.
- W2927763954 hasAuthorship W2927763954A5020462682 @default.
- W2927763954 hasAuthorship W2927763954A5025524753 @default.
- W2927763954 hasAuthorship W2927763954A5042861404 @default.
- W2927763954 hasConcept C14086860 @default.
- W2927763954 hasConcept C159654299 @default.
- W2927763954 hasConcept C185592680 @default.
- W2927763954 hasConcept C202751555 @default.
- W2927763954 hasConcept C203014093 @default.
- W2927763954 hasConcept C2776727803 @default.
- W2927763954 hasConcept C55493867 @default.
- W2927763954 hasConcept C86803240 @default.
- W2927763954 hasConcept C86807702 @default.
- W2927763954 hasConceptScore W2927763954C14086860 @default.
- W2927763954 hasConceptScore W2927763954C159654299 @default.
- W2927763954 hasConceptScore W2927763954C185592680 @default.
- W2927763954 hasConceptScore W2927763954C202751555 @default.
- W2927763954 hasConceptScore W2927763954C203014093 @default.
- W2927763954 hasConceptScore W2927763954C2776727803 @default.
- W2927763954 hasConceptScore W2927763954C55493867 @default.
- W2927763954 hasConceptScore W2927763954C86803240 @default.
- W2927763954 hasConceptScore W2927763954C86807702 @default.
- W2927763954 hasFunder F4320308126 @default.
- W2927763954 hasLocation W29277639541 @default.
- W2927763954 hasLocation W29277639542 @default.
- W2927763954 hasOpenAccess W2927763954 @default.
- W2927763954 hasPrimaryLocation W29277639541 @default.
- W2927763954 hasRelatedWork W1583822428 @default.
- W2927763954 hasRelatedWork W1856085589 @default.
- W2927763954 hasRelatedWork W1904422975 @default.
- W2927763954 hasRelatedWork W1962151629 @default.
- W2927763954 hasRelatedWork W2064155399 @default.
- W2927763954 hasRelatedWork W2927763954 @default.
- W2927763954 hasRelatedWork W2945891543 @default.
- W2927763954 hasRelatedWork W3047706242 @default.
- W2927763954 hasRelatedWork W4243087998 @default.
- W2927763954 hasRelatedWork W2074747632 @default.
- W2927763954 isParatext "false" @default.
- W2927763954 isRetracted "false" @default.
- W2927763954 magId "2927763954" @default.
- W2927763954 workType "article" @default.