Matches in SemOpenAlex for { <https://semopenalex.org/work/W2927884401> ?p ?o ?g. }
- W2927884401 endingPage "19" @default.
- W2927884401 startingPage "9" @default.
- W2927884401 abstract "Chordoma is a rare, slow-growing tumor thought to arise from remnants of embryonic notochord associated with an aggressive outcome. Cancer stem-like cells (CSCs) are related to tumorigenesis, recurrence, and resistance in cancers. Therefore, chordoma CSCs are possible targets for chordoma treatment. In this study, dysregulated miRNAs were determined in chordoma CSCs and identified their role in chordoma. Dysregulated miRNAs were determined via miRNA microarray and validated through qPCR. miRNAs were transiently transfected to the chordoma cell lines and their roles in proliferation, apoptosis, migration and invasion capacities and stem-like properties were identified. Finally, a relationship between clinicopathological features and dysregulated miRNAs has been evaluated among 21 chordoma patients. CD133+CD15+ cells exhibited CSC phenotype with increased CSC- and Epithelial-Mesenchymal Transition (EMT)-related gene expression, invasion, migration, tumorosphere- and colony-forming abilities. In addition, WNT5A, TGF-α, BTG2 and MYCBP genes involved in CSC-related pathways, were targets of miR-140-3p, miR-148a-3p, miR-210-5p and miR-574-5p, respectively. Transfection of CSC-related miRNAs also increased migration and invasion along with stem cell phenotype. Finally, we determined that miR-140-3p and miR-148a-3p expressions correlated with Ki67 while miR-140-3p and TGF-α expressions were correlated with p53. Moreover, MYCBP expression was positively correlated with tumor volume, and metastasis was associated with the expression of miR-210-5p and TGF-α in our patient cohort. Through these findings, we conclude that chordoma CSCs have distinctive miRNA profile, which can regulate stem-like properties of chordoma CSCs." @default.
- W2927884401 created "2019-04-11" @default.
- W2927884401 creator A5016529837 @default.
- W2927884401 creator A5020576934 @default.
- W2927884401 creator A5029961205 @default.
- W2927884401 creator A5037908808 @default.
- W2927884401 creator A5038751207 @default.
- W2927884401 creator A5048130296 @default.
- W2927884401 creator A5071418378 @default.
- W2927884401 creator A5078702707 @default.
- W2927884401 creator A5022536911 @default.
- W2927884401 date "2019-07-01" @default.
- W2927884401 modified "2023-10-18" @default.
- W2927884401 title "Distinctive role of dysregulated miRNAs in chordoma cancer stem-like cell maintenance" @default.
- W2927884401 cites W1503533733 @default.
- W2927884401 cites W1513788109 @default.
- W2927884401 cites W1563804318 @default.
- W2927884401 cites W1606979075 @default.
- W2927884401 cites W1611874876 @default.
- W2927884401 cites W1616948694 @default.
- W2927884401 cites W1724556032 @default.
- W2927884401 cites W1729209718 @default.
- W2927884401 cites W1963965802 @default.
- W2927884401 cites W1970135198 @default.
- W2927884401 cites W1986418246 @default.
- W2927884401 cites W1990521145 @default.
- W2927884401 cites W1991401267 @default.
- W2927884401 cites W1997280228 @default.
- W2927884401 cites W1997847812 @default.
- W2927884401 cites W2001746143 @default.
- W2927884401 cites W2007369931 @default.
- W2927884401 cites W2007852760 @default.
- W2927884401 cites W2008202172 @default.
- W2927884401 cites W2018243124 @default.
- W2927884401 cites W2022881840 @default.
- W2927884401 cites W2028188168 @default.
- W2927884401 cites W2028697507 @default.
- W2927884401 cites W2031182614 @default.
- W2927884401 cites W2035375527 @default.
- W2927884401 cites W2038543378 @default.
- W2927884401 cites W2040191060 @default.
- W2927884401 cites W2043510501 @default.
- W2927884401 cites W2050869737 @default.
- W2927884401 cites W2056908039 @default.
- W2927884401 cites W2076047768 @default.
- W2927884401 cites W2077251459 @default.
- W2927884401 cites W2083486480 @default.
- W2927884401 cites W2087962738 @default.
- W2927884401 cites W2089142809 @default.
- W2927884401 cites W2094073121 @default.
- W2927884401 cites W2100499682 @default.
- W2927884401 cites W2102085111 @default.
- W2927884401 cites W2102282192 @default.
- W2927884401 cites W2105175731 @default.
- W2927884401 cites W2108883574 @default.
- W2927884401 cites W2116908170 @default.
- W2927884401 cites W2118290727 @default.
- W2927884401 cites W2123250471 @default.
- W2927884401 cites W2134538447 @default.
- W2927884401 cites W2134987819 @default.
- W2927884401 cites W2143227861 @default.
- W2927884401 cites W2155386994 @default.
- W2927884401 cites W2161302946 @default.
- W2927884401 cites W2162143298 @default.
- W2927884401 cites W2163199119 @default.
- W2927884401 cites W2165286055 @default.
- W2927884401 cites W2310361607 @default.
- W2927884401 cites W2346775047 @default.
- W2927884401 cites W2480173037 @default.
- W2927884401 cites W2527997585 @default.
- W2927884401 cites W2548643352 @default.
- W2927884401 cites W2558443380 @default.
- W2927884401 cites W2580073784 @default.
- W2927884401 cites W2590272855 @default.
- W2927884401 cites W2591577802 @default.
- W2927884401 cites W2607128625 @default.
- W2927884401 cites W2736520162 @default.
- W2927884401 cites W2747864887 @default.
- W2927884401 cites W2757343106 @default.
- W2927884401 cites W2765811069 @default.
- W2927884401 cites W2766981404 @default.
- W2927884401 cites W2767596689 @default.
- W2927884401 cites W4235164524 @default.
- W2927884401 doi "https://doi.org/10.1016/j.yexcr.2019.03.039" @default.
- W2927884401 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30951707" @default.
- W2927884401 hasPublicationYear "2019" @default.
- W2927884401 type Work @default.
- W2927884401 sameAs 2927884401 @default.
- W2927884401 citedByCount "7" @default.
- W2927884401 countsByYear W29278844012019 @default.
- W2927884401 countsByYear W29278844012021 @default.
- W2927884401 countsByYear W29278844012022 @default.
- W2927884401 crossrefType "journal-article" @default.
- W2927884401 hasAuthorship W2927884401A5016529837 @default.
- W2927884401 hasAuthorship W2927884401A5020576934 @default.
- W2927884401 hasAuthorship W2927884401A5022536911 @default.
- W2927884401 hasAuthorship W2927884401A5029961205 @default.
- W2927884401 hasAuthorship W2927884401A5037908808 @default.