FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2928519906> ?p ?o ?g. }

• W2928519906 abstract "Abstract Human breast cancer is a malignant form of tumor with a relatively high mortality rate. Although esophageal cancer-related gene 4 (ECRG4) is thought to be a possible potent tumor suppressor gene that acts to suppress breast cancer, its precise role in this disease is not understood. Herein, we assess the correlation between ECRG4 expression and DNA methylation, probing the potential epigenetic regulation of ECRG4 in breast cancer. We analyzed ECRG4 promoter methylation via methylation-specific PCR (MSPCR), bisulfite sequencing, and a promoter reporter assay in human breast cancer cell lines and samples. Gene expression was assessed by quantitative real-time PCR (qPCR), while protein levels were assessed by Western blotting. CCK8 assays were used to quantify cell growth; Esophageal cancer-related gene 4 wound healing assays were used to assess cellular migration, while flow cytometry was used to assess apoptosis and cell cycle progression. Apoptosome formation was validated via CO-IP and Western blotting. We found that human breast cancer samples exhibited increased methylation of the ECRG4 promoter and decreased ECRG4 expression. Remarkably, the down-regulation of ECRG4 was highly associated with promoter methylation, and its expression could be re-activated via 5-aza-2′-deoxycytidine treatment to induce demethylation. ECRG4 overexpression impaired breast cancer cell proliferation and migration, and led to G0/G1 cell cycle phase arrest. Moreover, ECRG4 induced the formation of the Cytc/Apaf-1/caspase-9 apoptosome and promoted breast cancer cell apoptosis. ECRG4 is silenced in human breast cancer cells and cell lines, likely owing to promoter hypermethylation. ECRG4 may act as a tumor suppressor, inhibiting proliferation and migration, inducing G0/G1 phase arrest and apoptosis via the mitochondrial apoptotic pathway." @default.
• W2928519906 created "2019-04-11" @default.
• W2928519906 creator A5014894873 @default.
• W2928519906 creator A5018712272 @default.
• W2928519906 creator A5028008163 @default.
• W2928519906 creator A5038635573 @default.
• W2928519906 creator A5056248574 @default.
• W2928519906 creator A5068261372 @default.
• W2928519906 creator A5078938461 @default.
• W2928519906 creator A5084438362 @default.
• W2928519906 date "2019-05-01" @default.
• W2928519906 modified "2023-09-25" @default.
• W2928519906 title "ECRG4 acts as a tumor suppressor gene frequently hypermethylated in human breast cancer" @default.
• W2928519906 cites W1156671563 @default.
• W2928519906 cites W1500222335 @default.
• W2928519906 cites W1517637873 @default.
• W2928519906 cites W1550247880 @default.
• W2928519906 cites W1893419041 @default.
• W2928519906 cites W1965262464 @default.
• W2928519906 cites W1967690853 @default.
• W2928519906 cites W1968934960 @default.
• W2928519906 cites W1983858359 @default.
• W2928519906 cites W1985139403 @default.
• W2928519906 cites W1988583371 @default.
• W2928519906 cites W2000895026 @default.
• W2928519906 cites W2023371990 @default.
• W2928519906 cites W2034485619 @default.
• W2928519906 cites W2039764367 @default.
• W2928519906 cites W2060956825 @default.
• W2928519906 cites W2061212672 @default.
• W2928519906 cites W2062853648 @default.
• W2928519906 cites W2069188912 @default.
• W2928519906 cites W2074146931 @default.
• W2928519906 cites W2088540385 @default.
• W2928519906 cites W2089104774 @default.
• W2928519906 cites W2094129999 @default.
• W2928519906 cites W2097786201 @default.
• W2928519906 cites W2100185604 @default.
• W2928519906 cites W2103885797 @default.
• W2928519906 cites W2115464628 @default.
• W2928519906 cites W2119683782 @default.
• W2928519906 cites W2136959508 @default.
• W2928519906 cites W2163942900 @default.
• W2928519906 cites W2165541679 @default.
• W2928519906 cites W2170918883 @default.
• W2928519906 cites W2187447905 @default.
• W2928519906 cites W2237329788 @default.
• W2928519906 cites W2401930106 @default.
• W2928519906 cites W2405296431 @default.
• W2928519906 cites W2413649485 @default.
• W2928519906 cites W2736333399 @default.
• W2928519906 doi "https://doi.org/10.1042/bsr20190087" @default.
• W2928519906 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6509063" @default.
• W2928519906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30918105" @default.
• W2928519906 hasPublicationYear "2019" @default.
• W2928519906 type Work @default.
• W2928519906 sameAs 2928519906 @default.
• W2928519906 citedByCount "8" @default.
• W2928519906 countsByYear W29285199062020 @default.
• W2928519906 countsByYear W29285199062021 @default.
• W2928519906 countsByYear W29285199062022 @default.
• W2928519906 crossrefType "journal-article" @default.
• W2928519906 hasAuthorship W2928519906A5014894873 @default.
• W2928519906 hasAuthorship W2928519906A5018712272 @default.
• W2928519906 hasAuthorship W2928519906A5028008163 @default.
• W2928519906 hasAuthorship W2928519906A5038635573 @default.
• W2928519906 hasAuthorship W2928519906A5056248574 @default.
• W2928519906 hasAuthorship W2928519906A5068261372 @default.
• W2928519906 hasAuthorship W2928519906A5078938461 @default.
• W2928519906 hasAuthorship W2928519906A5084438362 @default.
• W2928519906 hasBestOaLocation W29285199061 @default.
• W2928519906 hasConcept C104317684 @default.
• W2928519906 hasConcept C121608353 @default.
• W2928519906 hasConcept C150194340 @default.
• W2928519906 hasConcept C153911025 @default.
• W2928519906 hasConcept C189235521 @default.
• W2928519906 hasConcept C190727270 @default.
• W2928519906 hasConcept C2778264664 @default.
• W2928519906 hasConcept C29537977 @default.
• W2928519906 hasConcept C33288867 @default.
• W2928519906 hasConcept C41091548 @default.
• W2928519906 hasConcept C502942594 @default.
• W2928519906 hasConcept C530470458 @default.
• W2928519906 hasConcept C54355233 @default.
• W2928519906 hasConcept C555283112 @default.
• W2928519906 hasConcept C62112901 @default.
• W2928519906 hasConcept C86803240 @default.
• W2928519906 hasConceptScore W2928519906C104317684 @default.
• W2928519906 hasConceptScore W2928519906C121608353 @default.
• W2928519906 hasConceptScore W2928519906C150194340 @default.
• W2928519906 hasConceptScore W2928519906C153911025 @default.
• W2928519906 hasConceptScore W2928519906C189235521 @default.
• W2928519906 hasConceptScore W2928519906C190727270 @default.
• W2928519906 hasConceptScore W2928519906C2778264664 @default.
• W2928519906 hasConceptScore W2928519906C29537977 @default.
• W2928519906 hasConceptScore W2928519906C33288867 @default.
• W2928519906 hasConceptScore W2928519906C41091548 @default.
• W2928519906 hasConceptScore W2928519906C502942594 @default.
• W2928519906 hasConceptScore W2928519906C530470458 @default.
• W2928519906 hasConceptScore W2928519906C54355233 @default.

• next