Matches in SemOpenAlex for { <https://semopenalex.org/work/W292871005> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W292871005 endingPage "56" @default.
- W292871005 startingPage "47" @default.
- W292871005 abstract "On inflamed endothelium the cell surface protein E-selectin isexpressed which supports the initial process of attachment -capturing and rolling of leukocytes. A recombinant CHO cell linesecreting a soluble E-selectin-IgG chimera was cultivated competitively under serum free conditions in three different bioreactor systems: a 1 l Super-Spinner, a 2 l stirred tank bioreactor equipped with a spinfilter, and a 100 l stirred tankbioreactor. In the smallest system 25.4 mg E-selectin-IgG wereproduced in 62 days using a repeated batch process whileachieving a maximal viable cell density of 3.7 x 10(6) cells ml(-1). Using continuous perfusion mode a total amount of35.2 mg were produced with a maximal viable cell density of1.65 x 10(7) cells ml(-1) in the 2 l bioreactor within 29 days. Large scale cultivation in a 100 l stirred tankbioreactor yielded 105.6 mg in three batches with a maximal viable cell density of 9.7 x 10(5) cells ml(-1) within 15 days. After removal of the cells by continuous centrifugation and a depth filter clearance step, the supernatants were concentrated via ultra filtration. Purificationwas performed by affinity chromatography with rProtein A. Integrity of the E-selectin-IgG protein was checked with SDS PAGE. Its activity was verified in a cellular adhesion assay performed with HL-60 cells and a recombinant CHO cell line expressing membrane-anchored E-selectin constitutively, and E-selectin expressing HUVECs, respectively. Soluble E-selectin-IgG was used to block adhesion to these cell layerscompetitively. A concentation of 18.8 and 37.5 mug ml(-1)was sufficient to reduce the amount of adhering HL-60 cells to 50% on CHO and HUVEC layers, respectively." @default.
- W292871005 created "2016-06-24" @default.
- W292871005 creator A5019635764 @default.
- W292871005 creator A5033253329 @default.
- W292871005 creator A5046935993 @default.
- W292871005 creator A5055260011 @default.
- W292871005 creator A5081078003 @default.
- W292871005 date "2002-01-01" @default.
- W292871005 modified "2023-09-24" @default.
- W292871005 cites W1988736620 @default.
- W292871005 cites W2048916873 @default.
- W292871005 cites W2057905142 @default.
- W292871005 cites W2093267654 @default.
- W292871005 cites W2094274802 @default.
- W292871005 cites W2123248116 @default.
- W292871005 cites W2128256270 @default.
- W292871005 cites W2143333940 @default.
- W292871005 cites W2235997788 @default.
- W292871005 cites W2298229328 @default.
- W292871005 cites W2470370844 @default.
- W292871005 cites W2576742884 @default.
- W292871005 doi "https://doi.org/10.1023/a:1021145813253" @default.
- W292871005 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3449925" @default.
- W292871005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19003086" @default.
- W292871005 hasPublicationYear "2002" @default.
- W292871005 type Work @default.
- W292871005 sameAs 292871005 @default.
- W292871005 citedByCount "4" @default.
- W292871005 countsByYear W2928710052014 @default.
- W292871005 countsByYear W2928710052017 @default.
- W292871005 crossrefType "journal-article" @default.
- W292871005 hasAuthorship W292871005A5019635764 @default.
- W292871005 hasAuthorship W292871005A5033253329 @default.
- W292871005 hasAuthorship W292871005A5046935993 @default.
- W292871005 hasAuthorship W292871005A5055260011 @default.
- W292871005 hasAuthorship W292871005A5081078003 @default.
- W292871005 hasBestOaLocation W2928710052 @default.
- W292871005 hasConcept C104317684 @default.
- W292871005 hasConcept C1491633281 @default.
- W292871005 hasConcept C153911025 @default.
- W292871005 hasConcept C168170006 @default.
- W292871005 hasConcept C178790620 @default.
- W292871005 hasConcept C179247698 @default.
- W292871005 hasConcept C181199279 @default.
- W292871005 hasConcept C185592680 @default.
- W292871005 hasConcept C40767141 @default.
- W292871005 hasConcept C43617362 @default.
- W292871005 hasConcept C54355233 @default.
- W292871005 hasConcept C55493867 @default.
- W292871005 hasConcept C7623868 @default.
- W292871005 hasConcept C81885089 @default.
- W292871005 hasConcept C84416704 @default.
- W292871005 hasConcept C85789140 @default.
- W292871005 hasConcept C86803240 @default.
- W292871005 hasConceptScore W292871005C104317684 @default.
- W292871005 hasConceptScore W292871005C1491633281 @default.
- W292871005 hasConceptScore W292871005C153911025 @default.
- W292871005 hasConceptScore W292871005C168170006 @default.
- W292871005 hasConceptScore W292871005C178790620 @default.
- W292871005 hasConceptScore W292871005C179247698 @default.
- W292871005 hasConceptScore W292871005C181199279 @default.
- W292871005 hasConceptScore W292871005C185592680 @default.
- W292871005 hasConceptScore W292871005C40767141 @default.
- W292871005 hasConceptScore W292871005C43617362 @default.
- W292871005 hasConceptScore W292871005C54355233 @default.
- W292871005 hasConceptScore W292871005C55493867 @default.
- W292871005 hasConceptScore W292871005C7623868 @default.
- W292871005 hasConceptScore W292871005C81885089 @default.
- W292871005 hasConceptScore W292871005C84416704 @default.
- W292871005 hasConceptScore W292871005C85789140 @default.
- W292871005 hasConceptScore W292871005C86803240 @default.
- W292871005 hasIssue "1/3" @default.
- W292871005 hasLocation W2928710051 @default.
- W292871005 hasLocation W2928710052 @default.
- W292871005 hasLocation W2928710053 @default.
- W292871005 hasLocation W2928710054 @default.
- W292871005 hasOpenAccess W292871005 @default.
- W292871005 hasPrimaryLocation W2928710051 @default.
- W292871005 hasRelatedWork W2038687882 @default.
- W292871005 hasRelatedWork W2354879164 @default.
- W292871005 hasRelatedWork W2364400598 @default.
- W292871005 hasRelatedWork W2368814887 @default.
- W292871005 hasRelatedWork W2369334130 @default.
- W292871005 hasRelatedWork W2373663781 @default.
- W292871005 hasRelatedWork W2390064643 @default.
- W292871005 hasRelatedWork W2392388241 @default.
- W292871005 hasRelatedWork W3026200651 @default.
- W292871005 hasRelatedWork W3030131892 @default.
- W292871005 hasVolume "38" @default.
- W292871005 isParatext "false" @default.
- W292871005 isRetracted "false" @default.
- W292871005 magId "292871005" @default.
- W292871005 workType "article" @default.