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- W292981689 abstract "SUMMARY The Mediterranean cereal cyst nematode, Heterodera latipons, attacks barley grown in the Northern and Southern Mediterranean phytogeographical zones and Eastern Desert of Jordan with variation in incidence and severity. The aim of this study was to clone a candidate parasitism gene from local populations of H. latipons. These targeted genes encode secretory protein products. Three populations of H. latipons from Ar-Ramtha, Madaba and Dana were identified. Morphological studies were used for all populations to confirm species identity before any molecular work. Pectate lyase and chorismate genes were the target genes. Therefore primers were designed based on alignment from H. latipons. Certain primer combinations showed amplicons using genomic DNA (directly from second-stage juveniles). A total of 35 amplicons were cloned and 18 of them were sent for sequencing. These clones were analysed using Blastx searches against sequences in NCBI databases. Blastx resulted in similarities to putative proteins such as digestive gland protein, glutamyl transpeptidedase and putative ubiquitin. The open reading frame of ubiquitin extension protein was 300 bp long. The cloned ubiquitin sequence from H. latipons has a high similarity to ubiquitin from Heterodera schachtii and H. glycines." @default.
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- W292981689 date "2009-01-01" @default.
- W292981689 modified "2023-09-27" @default.
- W292981689 title "CLONING OF PARASITISM GENES FROM THE MEDITERRANEAN CEREAL CYST NEMATODE, HETERODERA LATIPONS *" @default.
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