FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2931068> ?p ?o ?g. }

• W2931068 abstract "L ow-temperature Si-to-glass bonding using poly m e t hy l m e t h a c rylate (PMMA) as an a d h e s ive layer is developed to integrate electrodes with Si channels. The integr a t e d microsystem contains channels dry etched in Si with widths ranging from 3μm to 1 0 0μm and depths ranging from 100nm to 30μm. The channels are bonded to a 1 0 0μm-thick glass consisting of 600nm-thick patterned PMMA and 20/50nm-thick Cr/Au electrodes, with PMMA as an adhesive laye r. The typical bond strength is 3 M Pa, obtained by bonding at 110°C with 600nm-thick PMMA. Fluidic flow studies are carried out in channels that are 50μm and 100μm-wide with a depth of 100nm. Deionized water flows through the sealed Si channels due to capillary pressure with an initial velocity of 0.65mm/s for 50μm-wide and 100nm-deep channels. Electric fields are used to induce deoxyribonucleic acid (DNA) motion with velocities from 2.4μm to 14.5μm/s in 100μm-wide and 20μm-deep channels. The forces generated by the fields and the fluid flow are also used to stretch the tethered DNA molecules up to 1 5μm long in the microchannels. A technique called “protein-assisted DNA immobilization” (PADI) is deve l oped to immobilize and stretch, but not overstretch, DNA molecules inside a micro/nanochannel with limited surfa c e interactions while maintaining continuous hydration at p hy s i o l ogical pH. The biological activity of the immobilized DNA molecules is confi rmed by digesting the DNA with restriction enzymes in the microchannel. Singlemolecule transcription, which has stringent requirements on the immobilized DNA with respect to surface interactions and stretched lengths, is also successfully demonstrated on DNA molecules immobilized by PADI. In addition to arr aying DNA molecules for study of DNA protein interactions, the immobilization method could be used to construct DNA-templated nano-electronic dev i c e s . Control over the placement of stretched DNA molecules in a microfluidic system is a critical requirement for molecular nanotechnolog y. A technique is developed where a large number of DNA molecules can be immobilized s p e c i fi c a l ly at one end to the electrode tip and stretched in a microchannel using h i g h f r e q u e n cy ac fields. λD NA molecules are immobilized and stretched using 100kHz ac fields in a 100μm-wide and 75μm-deep Si microchannel. Using a floating electrode in between two biased electrodes, stretched T2 DNA molecules are immobilized across a 5μm-wide electrode gap by electric field and hydrodynamic flow. This project is supported by the National Science Fo u n d a t i o n under award number NSF-NIRT 0304316. Venkat Ram Dukkipati, Ji Hoon Kim, Ronald G. Larson, and Stella W. Pang" @default.
• W2931068 created "2016-06-24" @default.
• W2931068 creator A5004970118 @default.
• W2931068 creator A5007300789 @default.
• W2931068 creator A5029067826 @default.
• W2931068 creator A5057596349 @default.
• W2931068 creator A5088380778 @default.
• W2931068 date "2008-01-01" @default.
• W2931068 modified "2023-09-24" @default.
• W2931068 title "Stretching and Immobilization of DNA Molecules in Si Channels With Integrated Electrodes" @default.
• W2931068 hasPublicationYear "2008" @default.
• W2931068 type Work @default.
• W2931068 sameAs 2931068 @default.
• W2931068 citedByCount "0" @default.
• W2931068 crossrefType "journal-article" @default.
• W2931068 hasAuthorship W2931068A5004970118 @default.
• W2931068 hasAuthorship W2931068A5007300789 @default.
• W2931068 hasAuthorship W2931068A5029067826 @default.
• W2931068 hasAuthorship W2931068A5057596349 @default.
• W2931068 hasAuthorship W2931068A5088380778 @default.
• W2931068 hasConcept C113196181 @default.
• W2931068 hasConcept C121332964 @default.
• W2931068 hasConcept C147789679 @default.
• W2931068 hasConcept C151054161 @default.
• W2931068 hasConcept C159985019 @default.
• W2931068 hasConcept C171250308 @default.
• W2931068 hasConcept C17525397 @default.
• W2931068 hasConcept C178790620 @default.
• W2931068 hasConcept C185592680 @default.
• W2931068 hasConcept C192562407 @default.
• W2931068 hasConcept C196806460 @default.
• W2931068 hasConcept C32909587 @default.
• W2931068 hasConcept C43617362 @default.
• W2931068 hasConcept C60799052 @default.
• W2931068 hasConcept C62520636 @default.
• W2931068 hasConcept C8673954 @default.
• W2931068 hasConceptScore W2931068C113196181 @default.
• W2931068 hasConceptScore W2931068C121332964 @default.
• W2931068 hasConceptScore W2931068C147789679 @default.
• W2931068 hasConceptScore W2931068C151054161 @default.
• W2931068 hasConceptScore W2931068C159985019 @default.
• W2931068 hasConceptScore W2931068C171250308 @default.
• W2931068 hasConceptScore W2931068C17525397 @default.
• W2931068 hasConceptScore W2931068C178790620 @default.
• W2931068 hasConceptScore W2931068C185592680 @default.
• W2931068 hasConceptScore W2931068C192562407 @default.
• W2931068 hasConceptScore W2931068C196806460 @default.
• W2931068 hasConceptScore W2931068C32909587 @default.
• W2931068 hasConceptScore W2931068C43617362 @default.
• W2931068 hasConceptScore W2931068C60799052 @default.
• W2931068 hasConceptScore W2931068C62520636 @default.
• W2931068 hasConceptScore W2931068C8673954 @default.
• W2931068 hasLocation W29310681 @default.
• W2931068 hasOpenAccess W2931068 @default.
• W2931068 hasPrimaryLocation W29310681 @default.
• W2931068 hasRelatedWork W1985549771 @default.
• W2931068 hasRelatedWork W1995792009 @default.
• W2931068 hasRelatedWork W2003034046 @default.
• W2931068 hasRelatedWork W2006475027 @default.
• W2931068 hasRelatedWork W2007391088 @default.
• W2931068 hasRelatedWork W2012537287 @default.
• W2931068 hasRelatedWork W2018446538 @default.
• W2931068 hasRelatedWork W2025593246 @default.
• W2931068 hasRelatedWork W2035221024 @default.
• W2931068 hasRelatedWork W2036801673 @default.
• W2931068 hasRelatedWork W2046750136 @default.
• W2931068 hasRelatedWork W2051601244 @default.
• W2931068 hasRelatedWork W2065682928 @default.
• W2931068 hasRelatedWork W2077438223 @default.
• W2931068 hasRelatedWork W2119074693 @default.
• W2931068 hasRelatedWork W2240862080 @default.
• W2931068 hasRelatedWork W2396717514 @default.
• W2931068 hasRelatedWork W2417510895 @default.
• W2931068 hasRelatedWork W2619967697 @default.
• W2931068 hasRelatedWork W50950325 @default.
• W2931068 isParatext "false" @default.
• W2931068 isRetracted "false" @default.
• W2931068 magId "2931068" @default.
• W2931068 workType "article" @default.