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- W2932994889 abstract "Long noncoding RNAs (lncRNAs) play important roles in several processes including control of gene expression. These RNAs function through binding to histone-modifying complexes and transcriptional machinery including transcription factor, mediator, and RNA polymerase II. We present methods for the discovery and characterization of lncRNAs. RNA immunoprecipitation (RIP) is a modified version of chromatin immunoprecipitation (ChIP), and it is now generally used in lncRNA study. The method allows for testing of lncRNA-protein interactions in vivo. RIP assay facilitates the identification of consensus sequences of preferred binding site for the RNA-binding protein under study, and identification of the binding sites can provide valuable information on the possible mechanism by which the RNA-binding protein functions." @default.
- W2932994889 created "2019-04-11" @default.
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- W2932994889 date "2019-01-01" @default.
- W2932994889 modified "2023-09-23" @default.
- W2932994889 title "Analysis of Interaction Between Long Noncoding RNAs and Protein by RNA Immunoprecipitation in Arabidopsis" @default.
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- W2932994889 doi "https://doi.org/10.1007/978-1-4939-9045-0_18" @default.
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