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- W29337838 abstract "The chromatographic properties of a range of peptides varying in size from di- to decapeptide have been investigated by reversed-phase high-pressure liquid chromatography. A new set of conditions, namely, the addition of phosphoric acid to the mobile phase, has been found to have very real advantages in the analysis of underivatised peptides. These conditions allowed marked alterations in retention times, improvement in reproducibility and excellent resolution of peptides differing by as little as a single amino acid. A major advantage of phosphoric acid is that it can be used successfully in the range 195–220 nm which makes it compatible with the use of variable wavelength UV monitors as sensitive detectors in high-pressure liquid chromatography. In addition, the use of phosphoric acid permits the significant lowering of concentrations of organic solvents in the mobile phase, thus reducing the possibility of denaturation or precipitation." @default.
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- W29337838 date "1978-06-01" @default.
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- W29337838 title "High-pressure liquid chromatography of peptides and proteins" @default.
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- W29337838 doi "https://doi.org/10.1016/s0021-9673(00)95499-2" @default.
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