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- W2933970118 endingPage "2013" @default.
- W2933970118 startingPage "2000" @default.
- W2933970118 abstract "Neuromyelitis optica (NMO) is an autoimmune CNS disorder mediated by pathogenic aquaporin-4 (AQP4) water channel autoantibodies (AQP4-IgG). Although AQP4-IgG–driven complement-dependent cytotoxicity (CDC) is critical for the formation of NMO lesions, the molecular mechanisms governing optimal classical pathway activation are unknown. We investigated the molecular determinants driving CDC in NMO using recombinant AQP4–specific autoantibodies (AQP4 rAbs) derived from affected patients. We identified a group of AQP4 rAbs targeting a distinct extracellular loop C epitope that demonstrated enhanced CDC on target cells. Targeted mutations of AQP4 rAb Fc domains that enhance or diminish C1q binding or antibody Fc-Fc interactions showed that optimal CDC was driven by the assembly of multimeric rAb platforms that increase multivalent C1q binding and facilitate C1q activation. A peptide that blocks antibody Fc-Fc interaction inhibited CDC induced by AQP4 rAbs and polyclonal NMO patient sera. Super-resolution microscopy revealed that AQP4 rAbs with enhanced CDC preferentially formed organized clusters on supramolecular AQP4 orthogonal arrays, linking epitope-dependent multimeric assembly with enhanced C1q binding and activation. The resulting model of AQP4-IgG CDC provides a framework for understanding classical complement activation in human autoantibody–mediated disorders and identifies a potential new therapeutic avenue for treating NMO." @default.
- W2933970118 created "2019-04-11" @default.
- W2933970118 creator A5016397545 @default.
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- W2933970118 creator A5082010009 @default.
- W2933970118 date "2019-04-08" @default.
- W2933970118 modified "2023-10-14" @default.
- W2933970118 title "Membrane assembly of aquaporin-4 autoantibodies regulates classical complement activation in neuromyelitis optica" @default.
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- W2933970118 doi "https://doi.org/10.1172/jci122942" @default.
- W2933970118 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6486354" @default.
- W2933970118 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30958797" @default.
- W2933970118 hasPublicationYear "2019" @default.
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