Matches in SemOpenAlex for { <https://semopenalex.org/work/W2933973347> ?p ?o ?g. }
- W2933973347 abstract "Abstract Insect chitinases are necessary hydrolytic enzymes for chitin degradation, insect molting and metamorphosis. In this study, five chitinases encoded in the genome of Spodoptera exigua (SeCHIT7, SeCHIT11, SeCHIT12, SeCHIT13 and SeCHIT14) were identified by reverse transcription PCR. Phylogenetic analysis indicated that SeCHIT7 belonged to Group III, and SeCHIT11/12/13/14 belonged to Group VIII. Real-time quantitative PCR analyses showed that SeCHIT7 had an extensive transcription at the fourth- and fifth-instar larval and pupal stages, while SeCHIT11 had the highest transcription level at the egg stage, and the transcription of SeCHIT12 increased by over 1000 times from pre-pupal to pupal stage. Tissue-specific analyses showed that these three SeCHITs (SeCHIT7, SeCHIT11 and SeCHIT12) were mainly transcribed in the midgut and fat body. Chitinase activity assays suggested that the activity had a lower level at the egg stage and peaked at the pupal stage. The activities were increased by 9.4 times from egg to first-instar larval stage. Tissue specificity analysis showed that the highest activity was observed in the fat body, followed by hemolymph and cuticle of the pre-pupal stage. Overall, these results provided valid information for further research on the biological function and regulation of chitinases in S. exigua." @default.
- W2933973347 created "2019-04-11" @default.
- W2933973347 creator A5020533068 @default.
- W2933973347 creator A5020656201 @default.
- W2933973347 creator A5024561648 @default.
- W2933973347 creator A5039009774 @default.
- W2933973347 creator A5067852346 @default.
- W2933973347 creator A5075764723 @default.
- W2933973347 creator A5087717403 @default.
- W2933973347 date "2019-06-01" @default.
- W2933973347 modified "2023-10-18" @default.
- W2933973347 title "Molecular characterization, activity analysis and transcriptional detection of chitinases encoded in the genome of Spodoptera exigua" @default.
- W2933973347 cites W103430187 @default.
- W2933973347 cites W1527301642 @default.
- W2933973347 cites W1549352522 @default.
- W2933973347 cites W1706498001 @default.
- W2933973347 cites W1718799863 @default.
- W2933973347 cites W1862572695 @default.
- W2933973347 cites W1963957860 @default.
- W2933973347 cites W1965374557 @default.
- W2933973347 cites W1966018313 @default.
- W2933973347 cites W1977922844 @default.
- W2933973347 cites W1978199420 @default.
- W2933973347 cites W1979022108 @default.
- W2933973347 cites W1984036556 @default.
- W2933973347 cites W1985796363 @default.
- W2933973347 cites W1990444531 @default.
- W2933973347 cites W2012988025 @default.
- W2933973347 cites W2013290668 @default.
- W2933973347 cites W2021851085 @default.
- W2933973347 cites W2035738763 @default.
- W2933973347 cites W2040368647 @default.
- W2933973347 cites W2044245203 @default.
- W2933973347 cites W2054211958 @default.
- W2933973347 cites W2054901867 @default.
- W2933973347 cites W2057880096 @default.
- W2933973347 cites W2060436495 @default.
- W2933973347 cites W2069049656 @default.
- W2933973347 cites W2075364689 @default.
- W2933973347 cites W2085836042 @default.
- W2933973347 cites W2093332482 @default.
- W2933973347 cites W2094974659 @default.
- W2933973347 cites W2095669939 @default.
- W2933973347 cites W2096814446 @default.
- W2933973347 cites W2107277218 @default.
- W2933973347 cites W2108276490 @default.
- W2933973347 cites W2112814626 @default.
- W2933973347 cites W2133431389 @default.
- W2933973347 cites W2134603452 @default.
- W2933973347 cites W2151957037 @default.
- W2933973347 cites W2170342983 @default.
- W2933973347 cites W2180072250 @default.
- W2933973347 cites W2204805509 @default.
- W2933973347 cites W2277248045 @default.
- W2933973347 cites W2311203695 @default.
- W2933973347 cites W2604025696 @default.
- W2933973347 cites W2794509736 @default.
- W2933973347 doi "https://doi.org/10.1016/j.aspen.2019.03.007" @default.
- W2933973347 hasPublicationYear "2019" @default.
- W2933973347 type Work @default.
- W2933973347 sameAs 2933973347 @default.
- W2933973347 citedByCount "2" @default.
- W2933973347 countsByYear W29339733472020 @default.
- W2933973347 countsByYear W29339733472021 @default.
- W2933973347 crossrefType "journal-article" @default.
- W2933973347 hasAuthorship W2933973347A5020533068 @default.
- W2933973347 hasAuthorship W2933973347A5020656201 @default.
- W2933973347 hasAuthorship W2933973347A5024561648 @default.
- W2933973347 hasAuthorship W2933973347A5039009774 @default.
- W2933973347 hasAuthorship W2933973347A5067852346 @default.
- W2933973347 hasAuthorship W2933973347A5075764723 @default.
- W2933973347 hasAuthorship W2933973347A5087717403 @default.
- W2933973347 hasConcept C100068826 @default.
- W2933973347 hasConcept C104317684 @default.
- W2933973347 hasConcept C136397037 @default.
- W2933973347 hasConcept C138885662 @default.
- W2933973347 hasConcept C150149183 @default.
- W2933973347 hasConcept C153911025 @default.
- W2933973347 hasConcept C173758957 @default.
- W2933973347 hasConcept C175734852 @default.
- W2933973347 hasConcept C179926584 @default.
- W2933973347 hasConcept C2777612826 @default.
- W2933973347 hasConcept C2777773057 @default.
- W2933973347 hasConcept C2778457844 @default.
- W2933973347 hasConcept C2779732960 @default.
- W2933973347 hasConcept C2780778831 @default.
- W2933973347 hasConcept C2781088250 @default.
- W2933973347 hasConcept C40767141 @default.
- W2933973347 hasConcept C41895202 @default.
- W2933973347 hasConcept C41905685 @default.
- W2933973347 hasConcept C54355233 @default.
- W2933973347 hasConcept C55493867 @default.
- W2933973347 hasConcept C59822182 @default.
- W2933973347 hasConcept C86803240 @default.
- W2933973347 hasConceptScore W2933973347C100068826 @default.
- W2933973347 hasConceptScore W2933973347C104317684 @default.
- W2933973347 hasConceptScore W2933973347C136397037 @default.
- W2933973347 hasConceptScore W2933973347C138885662 @default.
- W2933973347 hasConceptScore W2933973347C150149183 @default.
- W2933973347 hasConceptScore W2933973347C153911025 @default.