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• W2934303500 abstract "The pentose phosphate pathway (PPP) is essential for reductive biosynthesis, antioxidant processes and nucleotide production. Common tracers such as [1,2-13 C2 ]glucose rely on detection of 13 C in lactate and require assumptions to correct natural 13 C abundance. Here, we introduce a novel and specific tracer of the PPP, [2,3-13 C2 ]glucose. 13 C NMR analysis of the resulting isotopomers is informative because [1,2-13 C2 ]lactate arises from glycolysis and [2,3-13 C2 ]lactate arises exclusively through the PPP. A correction for natural abundance is unnecessary. In rats receiving [2,3-13 C2 ]glucose, the PPP was more active in the fed versus fasted state in the liver and the heart, consistent with increased expression of key enzymes in the PPP. Both the PPP and glycolysis were substantially increased in hepatoma compared with liver. In summary, [2,3-13 C2 ]glucose and 13 C NMR simplify assessment of the PPP." @default.
• W2934303500 created "2019-04-11" @default.
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• W2934303500 date "2019-03-29" @default.
• W2934303500 modified "2023-10-17" @default.
• W2934303500 title "Assessing the pentose phosphate pathway using [2, 3‐¹³C₂]glucose" @default.
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• W2934303500 doi "https://doi.org/10.1002/nbm.4096" @default.
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