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• W2935070157 abstract "Abstract We used high-throughput flow cytometry to assess the ability of individual gene knockout strains of E coli to take up two membrane-permeable, cationic fluorescent dyes, viz the carbocyanine diS-C3(5) and the DNA dye SYBR Green. Individual strains showed a large range of distributions of uptake. The range of modal steady-state uptakes for the carbocyanine between the different strains was 36-fold. Knockouts of the ATP synthase α- and β-subunits greatly inhibited uptake, implying that most uptake was ATP-driven rather than being driven by say a membrane potential. Dozens of transporters changed the steady-state uptake of the dye by more than 50% with respect to that of the wild type, in both directions (increased or decreased); knockouts in known influx and efflux transporters behaved as expected, giving confidence in the general strategy. Many of the knockouts with the most reduced uptake were transporter genes of unknown function (‘y-genes’). Similarly, several overexpression variants in the ‘ASKA’ collection had the anticipated, opposite effects. Similar findings were made with SYBR Green (the range being some 69-fold), though despite it too containing a benzimidazole motif there was negligible correlation between its uptake and that of the carbocyanine when compared across the various strains. Overall, we conclude that the uptake of these dyes may be catalysed by a great many transporters of possibly broad and presently unknown specificity. This casts serious doubt upon the use of such dyes as quantitative stains for representing either bioenergetic parameters or the amount of cellular DNA in unfixed cells ( in vivo ). By contrast, it opens up their potential use as transporter assay substrates in high-throughput screening." @default.
• W2935070157 created "2019-04-11" @default.
• W2935070157 creator A5035399790 @default.
• W2935070157 creator A5054081363 @default.
• W2935070157 creator A5073714524 @default.
• W2935070157 creator A5077370611 @default.
• W2935070157 date "2019-04-09" @default.
• W2935070157 modified "2023-09-24" @default.
• W2935070157 title "Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes byEscherichia coli" @default.
• W2935070157 cites W125240721 @default.
• W2935070157 cites W139770000 @default.
• W2935070157 cites W145906557 @default.
• W2935070157 cites W156116736 @default.
• W2935070157 cites W1589712702 @default.
• W2935070157 cites W1594329622 @default.
• W2935070157 cites W163666902 @default.
• W2935070157 cites W1664609600 @default.
• W2935070157 cites W1665675542 @default.
• W2935070157 cites W1749004182 @default.
• W2935070157 cites W1785018154 @default.
• W2935070157 cites W1820155366 @default.
• W2935070157 cites W1837827509 @default.
• W2935070157 cites W1838367913 @default.
• W2935070157 cites W1942975626 @default.
• W2935070157 cites W1964812394 @default.
• W2935070157 cites W1965611124 @default.
• W2935070157 cites W1966098173 @default.
• W2935070157 cites W1966303539 @default.
• W2935070157 cites W1968258605 @default.
• W2935070157 cites W1972720706 @default.
• W2935070157 cites W1975704497 @default.
• W2935070157 cites W1977475313 @default.
• W2935070157 cites W1980381675 @default.
• W2935070157 cites W1981560730 @default.
• W2935070157 cites W1982211257 @default.
• W2935070157 cites W1982858965 @default.
• W2935070157 cites W1988149100 @default.
• W2935070157 cites W1988691705 @default.
• W2935070157 cites W1992578437 @default.
• W2935070157 cites W1993294422 @default.
• W2935070157 cites W1996093630 @default.
• W2935070157 cites W1998808597 @default.
• W2935070157 cites W1999979510 @default.
• W2935070157 cites W2000324747 @default.
• W2935070157 cites W2000640603 @default.
• W2935070157 cites W2007042991 @default.
• W2935070157 cites W2007236728 @default.
• W2935070157 cites W2008127220 @default.
• W2935070157 cites W2008410344 @default.
• W2935070157 cites W2009089119 @default.
• W2935070157 cites W2010043115 @default.
• W2935070157 cites W2014346478 @default.
• W2935070157 cites W2016075837 @default.
• W2935070157 cites W2018086140 @default.
• W2935070157 cites W2019081081 @default.
• W2935070157 cites W2029764374 @default.
• W2935070157 cites W2030373857 @default.
• W2935070157 cites W2031429688 @default.
• W2935070157 cites W2032054300 @default.
• W2935070157 cites W2034342778 @default.
• W2935070157 cites W2037152176 @default.
• W2935070157 cites W2039831645 @default.
• W2935070157 cites W2041956479 @default.
• W2935070157 cites W2042072026 @default.
• W2935070157 cites W2043110235 @default.
• W2935070157 cites W2051313468 @default.
• W2935070157 cites W2052463242 @default.
• W2935070157 cites W2052607700 @default.
• W2935070157 cites W2054585308 @default.
• W2935070157 cites W2057000572 @default.
• W2935070157 cites W2057617446 @default.
• W2935070157 cites W2058292418 @default.
• W2935070157 cites W2058668911 @default.
• W2935070157 cites W2063155289 @default.
• W2935070157 cites W2065652119 @default.
• W2935070157 cites W2065942389 @default.
• W2935070157 cites W2067035804 @default.
• W2935070157 cites W2069828457 @default.
• W2935070157 cites W2070624826 @default.
• W2935070157 cites W2071722462 @default.
• W2935070157 cites W2072495931 @default.
• W2935070157 cites W2073188874 @default.
• W2935070157 cites W2073762710 @default.
• W2935070157 cites W2074692830 @default.
• W2935070157 cites W2077142764 @default.
• W2935070157 cites W2078776981 @default.
• W2935070157 cites W2079079400 @default.
• W2935070157 cites W2079111596 @default.
• W2935070157 cites W2086941118 @default.
• W2935070157 cites W2087034160 @default.
• W2935070157 cites W2088095424 @default.
• W2935070157 cites W2088890481 @default.
• W2935070157 cites W2093020725 @default.
• W2935070157 cites W2096503523 @default.
• W2935070157 cites W2097559461 @default.
• W2935070157 cites W2098164808 @default.
• W2935070157 cites W2099811855 @default.
• W2935070157 cites W2099997130 @default.
• W2935070157 cites W2105979237 @default.
• W2935070157 cites W2107250776 @default.

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