Matches in SemOpenAlex for { <https://semopenalex.org/work/W2935837240> ?p ?o ?g. }
- W2935837240 endingPage "302" @default.
- W2935837240 startingPage "292" @default.
- W2935837240 abstract "Shaggy button weed (Spermacoce hispida L.) is a therapeutically important herb widely used in Indian System of medicine particularly Ayurvedic, Siddha, and Unani. S. hispida is recalcitrant during germination in natural habitat. Plant tissue culture is an alternate approach for mass multiplication of such vulnerable plants. Plant regeneration by organogenesis and somatic embryogenesis in S. hispida was attempted using cotyledonary leaf, shoot tip, hypocotyl, and nodal explants. Maximum shoot regeneration frequency (72%) and number of shoots (10.5 ± 0.3) through direct organogenesis was achieved from nodal explants cultured on Murashige and Skoog (MS) medium amended with 2.0 mg/dm3 of 6-benzylaminopurine (BAP). Further, MS medium containing 0.5 mg/dm3 2, 4-dichlorophenoxyacetic acid (2, 4-D) produced higher frequency of callus induction (86.25%) from hypocotyl segments. Optimum shoot regeneration response (69.5%) through indirect organogenesis was observed from hypocotyl-derived callus on MS medium fortified with 1.0 mg/dm3 kinetin with 6.1 shoots per callus. The highest regeneration frequency (68%) through somatic embryogenesis was recorded from nodal explants on MS medium supplemented with 2.0 mg/dm3 BAP and 0.8 mg/dm3 kinetin. The regenerated plantlets were acclimatized successfully under ex vitro conditions with normal phenotypic appearance. Shoot tips and nodes did not induce organogenesis (indirect), whereas nodal segments showed better regeneration via organogenesis (direct) and somatic embryogenesis in MS medium with either BAP or BAP with kinetin. Histological examinations confirmed the distinctive developmental stages and bipolar structure of somatic embryos. The described regeneration protocols can be effectively utilized for the large-scale multiplication, germplasm conservation, pharmacological and genetic manipulation studies." @default.
- W2935837240 created "2019-04-25" @default.
- W2935837240 creator A5005703042 @default.
- W2935837240 creator A5013181761 @default.
- W2935837240 creator A5017727548 @default.
- W2935837240 creator A5027013421 @default.
- W2935837240 creator A5065115245 @default.
- W2935837240 creator A5071360037 @default.
- W2935837240 date "2019-08-01" @default.
- W2935837240 modified "2023-09-25" @default.
- W2935837240 title "Efficient plant regeneration and histological evaluations of regenerants through organogenesis and somatic embryogenesis in Spermacoce hispida L.—An underutilized medicinally important plant" @default.
- W2935837240 cites W1231832268 @default.
- W2935837240 cites W1524819834 @default.
- W2935837240 cites W1851293620 @default.
- W2935837240 cites W1871755502 @default.
- W2935837240 cites W1936658659 @default.
- W2935837240 cites W1965297832 @default.
- W2935837240 cites W1968133765 @default.
- W2935837240 cites W1978911675 @default.
- W2935837240 cites W1985655450 @default.
- W2935837240 cites W1992716708 @default.
- W2935837240 cites W1994693652 @default.
- W2935837240 cites W2008066908 @default.
- W2935837240 cites W2009201493 @default.
- W2935837240 cites W2021317274 @default.
- W2935837240 cites W2024556021 @default.
- W2935837240 cites W2050599811 @default.
- W2935837240 cites W2079516192 @default.
- W2935837240 cites W2080061763 @default.
- W2935837240 cites W2080102818 @default.
- W2935837240 cites W2084233797 @default.
- W2935837240 cites W2085809310 @default.
- W2935837240 cites W2086061000 @default.
- W2935837240 cites W2087494182 @default.
- W2935837240 cites W2090605902 @default.
- W2935837240 cites W2091270156 @default.
- W2935837240 cites W2095426486 @default.
- W2935837240 cites W2105255550 @default.
- W2935837240 cites W2110847867 @default.
- W2935837240 cites W2119641358 @default.
- W2935837240 cites W2122322197 @default.
- W2935837240 cites W2140081980 @default.
- W2935837240 cites W2159497988 @default.
- W2935837240 cites W2169925604 @default.
- W2935837240 cites W2172597612 @default.
- W2935837240 cites W2174313637 @default.
- W2935837240 cites W2177586999 @default.
- W2935837240 cites W2199353895 @default.
- W2935837240 cites W2474921664 @default.
- W2935837240 cites W2571151592 @default.
- W2935837240 cites W2747172802 @default.
- W2935837240 cites W4250499346 @default.
- W2935837240 cites W1964331988 @default.
- W2935837240 doi "https://doi.org/10.1016/j.indcrop.2019.03.067" @default.
- W2935837240 hasPublicationYear "2019" @default.
- W2935837240 type Work @default.
- W2935837240 sameAs 2935837240 @default.
- W2935837240 citedByCount "4" @default.
- W2935837240 countsByYear W29358372402020 @default.
- W2935837240 countsByYear W29358372402022 @default.
- W2935837240 countsByYear W29358372402023 @default.
- W2935837240 crossrefType "journal-article" @default.
- W2935837240 hasAuthorship W2935837240A5005703042 @default.
- W2935837240 hasAuthorship W2935837240A5013181761 @default.
- W2935837240 hasAuthorship W2935837240A5017727548 @default.
- W2935837240 hasAuthorship W2935837240A5027013421 @default.
- W2935837240 hasAuthorship W2935837240A5065115245 @default.
- W2935837240 hasAuthorship W2935837240A5071360037 @default.
- W2935837240 hasConcept C104317684 @default.
- W2935837240 hasConcept C105409712 @default.
- W2935837240 hasConcept C157890192 @default.
- W2935837240 hasConcept C171056886 @default.
- W2935837240 hasConcept C200806820 @default.
- W2935837240 hasConcept C201468095 @default.
- W2935837240 hasConcept C202751555 @default.
- W2935837240 hasConcept C21410773 @default.
- W2935837240 hasConcept C2776499888 @default.
- W2935837240 hasConcept C30503417 @default.
- W2935837240 hasConcept C55493867 @default.
- W2935837240 hasConcept C59822182 @default.
- W2935837240 hasConcept C86803240 @default.
- W2935837240 hasConcept C95444343 @default.
- W2935837240 hasConcept C9549007 @default.
- W2935837240 hasConceptScore W2935837240C104317684 @default.
- W2935837240 hasConceptScore W2935837240C105409712 @default.
- W2935837240 hasConceptScore W2935837240C157890192 @default.
- W2935837240 hasConceptScore W2935837240C171056886 @default.
- W2935837240 hasConceptScore W2935837240C200806820 @default.
- W2935837240 hasConceptScore W2935837240C201468095 @default.
- W2935837240 hasConceptScore W2935837240C202751555 @default.
- W2935837240 hasConceptScore W2935837240C21410773 @default.
- W2935837240 hasConceptScore W2935837240C2776499888 @default.
- W2935837240 hasConceptScore W2935837240C30503417 @default.
- W2935837240 hasConceptScore W2935837240C55493867 @default.
- W2935837240 hasConceptScore W2935837240C59822182 @default.
- W2935837240 hasConceptScore W2935837240C86803240 @default.
- W2935837240 hasConceptScore W2935837240C95444343 @default.
- W2935837240 hasConceptScore W2935837240C9549007 @default.