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- W2936010324 abstract "The detection and characterization of chemical adducts on proteins is of increasing interest. Here, we described a step-by-step procedure to identify unknown chemical adduct modifications on proteins resulting from the interaction with a given reactive compound. The protocol can be divided into two equally important parts: (1) the wet laboratory work, to produce high quality mass spectrometry (MS) data of in vitro modified proteins and (2) the dry laboratory work, to analyze the generated MS data and provide highly confident qualitative and quantitative results on the chemical composition and amino acid localization of adducts. This protocol is applicable to the study of any pharmaceutical or chemical compound forming covalent protein adducts, detectable in LC-MS/MS experiments." @default.
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- W2936010324 date "2019-01-01" @default.
- W2936010324 modified "2023-10-02" @default.
- W2936010324 title "Detection of Unknown Chemical Adduct Modifications on Proteins: From Wet to Dry Laboratory" @default.
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- W2936010324 doi "https://doi.org/10.1007/978-1-4939-9232-4_8" @default.
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