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• W2936052783 abstract "Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq platform for scRNA-seq. We generate a resource of 468 single cells and 1297 matched single cDNA samples, performing SMARTer and Smart-seq2 protocols on two cell lines with RNA spike-ins. We sequence these libraries on both platforms using single- and paired-end reads. The platforms have comparable sensitivity and accuracy in terms of quantification of gene expression, and low technical variability. Our study provides a standardized scRNA-seq resource to benchmark new scRNA-seq library preparation protocols and sequencing platforms." @default.
• W2936052783 created "2019-04-25" @default.
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• W2936052783 date "2019-04-09" @default.
• W2936052783 modified "2023-10-16" @default.
• W2936052783 title "Comparative analysis of sequencing technologies for single-cell transcriptomics" @default.
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• W2936052783 doi "https://doi.org/10.1186/s13059-019-1676-5" @default.
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