FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2936190368> ?p ?o ?g. }

• W2936190368 abstract "Abstract Background Colorectal cancer (CRC) is still a leading cause of death worldwide. Recent studies have pointed to an important role of microRNAs carcinogenesis. In fact, several microRNAs have been described as aberrantly expressed in CRC tissues and in the serum of patients. More specifically, microRNAs with dual roles in both cancer and stem cell survival represent a potential source of novel molecular targets in CRC due to their described functions in normal and deregulated proliferation. However, the functional outcomes of microRNA aberrant expression still need to be explored at the cellular level. Here, we aimed to investigate the effects of microRNAs involved in the control of pluripotency of stem cells in the proliferation and cell death of a colorectal cancer cell line. Methods We performed transfection of 31 microRNA mimics in HCT116 CRC cells. Cell proliferation and cell death were measured after 4 days of treatment using fluorescence staining in a high content screening platform. Total number of live and dead cells were automatically counted and analyzed. To reveal mRNA targets, we used an oligonucleotide microarray. Functional classification of targets was done using DAVID tool. Gene expression of potential mRNA targets was performed by qPCR. Results Twenty microRNAs altered the proliferation of HCT116 cells in comparison to control. Three microRNAs significantly repressed cell proliferation and induced cell death simultaneously (miR-22-3p, miR-24-3p, and miR-101-3p). Interestingly, all anti-proliferative microRNAs in our study had been previously described as poorly expressed in the CRC samples and were implicated in the disease. Microarray analysis of miR-101-3p targets revealed Wnt and cancer as pathways regulated by this microRNA. Specific repression of anti-apoptotic isoform of MCL-1, a member of the BCL-2 family, was also identified as a possible mechanism for miR-101-3p anti-proliferative/pro-apoptotic effect. Conclusions microRNAs described as upregulated in CRC tend to induce proliferation in vitro, whereas microRNAs described as poorly expressed in CRC halt proliferation and induce cell death in vitro. Selective inhibition of anti-apoptotic MCL-1 contributes to anti-tumoral activity of miR-101-3p." @default.
• W2936190368 created "2019-04-25" @default.
• W2936190368 creator A5007587630 @default.
• W2936190368 creator A5017977553 @default.
• W2936190368 creator A5035206619 @default.
• W2936190368 creator A5057149624 @default.
• W2936190368 creator A5071045551 @default.
• W2936190368 creator A5075393732 @default.
• W2936190368 date "2019-04-10" @default.
• W2936190368 modified "2023-09-23" @default.
• W2936190368 title "Focused screening reveals functional effects of microRNAs differentially expressed in colorectal cancer" @default.
• W2936190368 cites W1496125310 @default.
• W2936190368 cites W1515008413 @default.
• W2936190368 cites W1523237749 @default.
• W2936190368 cites W1648301895 @default.
• W2936190368 cites W1744683414 @default.
• W2936190368 cites W1971588341 @default.
• W2936190368 cites W1982254984 @default.
• W2936190368 cites W1992970284 @default.
• W2936190368 cites W1997751561 @default.
• W2936190368 cites W2022143863 @default.
• W2936190368 cites W2023907252 @default.
• W2936190368 cites W2026982091 @default.
• W2936190368 cites W2028483180 @default.
• W2936190368 cites W2028561445 @default.
• W2936190368 cites W2031621381 @default.
• W2936190368 cites W2032298477 @default.
• W2936190368 cites W2038412530 @default.
• W2936190368 cites W2044782990 @default.
• W2936190368 cites W2045406210 @default.
• W2936190368 cites W2045705662 @default.
• W2936190368 cites W2046013132 @default.
• W2936190368 cites W2048183749 @default.
• W2936190368 cites W2059746106 @default.
• W2936190368 cites W2062380899 @default.
• W2936190368 cites W2068013806 @default.
• W2936190368 cites W2068918704 @default.
• W2936190368 cites W2071773142 @default.
• W2936190368 cites W2078786772 @default.
• W2936190368 cites W2080330299 @default.
• W2936190368 cites W2083381199 @default.
• W2936190368 cites W2086996124 @default.
• W2936190368 cites W2087695373 @default.
• W2936190368 cites W2093148531 @default.
• W2936190368 cites W2098055602 @default.
• W2936190368 cites W2113551318 @default.
• W2936190368 cites W2120109712 @default.
• W2936190368 cites W2122683221 @default.
• W2936190368 cites W2128796736 @default.
• W2936190368 cites W2133968430 @default.
• W2936190368 cites W2139528114 @default.
• W2936190368 cites W2141759915 @default.
• W2936190368 cites W2146512944 @default.
• W2936190368 cites W2146642335 @default.
• W2936190368 cites W2150536104 @default.
• W2936190368 cites W2153056550 @default.
• W2936190368 cites W2154294588 @default.
• W2936190368 cites W2154312575 @default.
• W2936190368 cites W2161191622 @default.
• W2936190368 cites W2162951368 @default.
• W2936190368 cites W2166343992 @default.
• W2936190368 cites W2169145911 @default.
• W2936190368 cites W2172182613 @default.
• W2936190368 cites W2191718196 @default.
• W2936190368 cites W2213904157 @default.
• W2936190368 cites W2258188776 @default.
• W2936190368 cites W2314360422 @default.
• W2936190368 cites W2339467330 @default.
• W2936190368 cites W2346347145 @default.
• W2936190368 cites W2413555286 @default.
• W2936190368 cites W2472885775 @default.
• W2936190368 cites W2482634524 @default.
• W2936190368 cites W2523322270 @default.
• W2936190368 cites W2550276615 @default.
• W2936190368 cites W2565777427 @default.
• W2936190368 cites W2579984573 @default.
• W2936190368 cites W2585895970 @default.
• W2936190368 cites W2588900011 @default.
• W2936190368 cites W2603978453 @default.
• W2936190368 cites W2607089568 @default.
• W2936190368 cites W2609081897 @default.
• W2936190368 cites W2617969189 @default.
• W2936190368 cites W2736518463 @default.
• W2936190368 cites W2737985553 @default.
• W2936190368 cites W2739102870 @default.
• W2936190368 cites W2741764772 @default.
• W2936190368 cites W2743923431 @default.
• W2936190368 cites W2765908113 @default.
• W2936190368 cites W2766270950 @default.
• W2936190368 cites W2767546566 @default.
• W2936190368 cites W2768724126 @default.
• W2936190368 cites W2781525129 @default.
• W2936190368 cites W2784138229 @default.
• W2936190368 cites W2784351052 @default.
• W2936190368 cites W2791570871 @default.
• W2936190368 cites W2803342670 @default.
• W2936190368 cites W2808927630 @default.
• W2936190368 cites W2895790751 @default.
• W2936190368 cites W2897900510 @default.
• W2936190368 cites W4213257593 @default.

• next