FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2936705082> ?p ?o ?g. }

• W2936705082 endingPage "2324" @default.
• W2936705082 startingPage "2315" @default.
• W2936705082 abstract "Adoptive transfer of regulatory T cells (FOXP3+ Tregs) has been developed as a potential curative immune therapy to prevent and treat autoimmune and graft-versus-host diseases (GVHD). A major limitation that has hindered the use of Treg immunotherapy in humans is the difficulty of consistently isolating and obtaining highly purified Tregs after ex vivo expansion. Methods: We isolated bona fide Tregs from expansion cultures based on their selective surface expression of latency-associated peptide (LAP). The TCR Vβ diversity and intracellular cytokine production of Tregs were determined by flow cytometer. The TSDR methylation was determined by epigenetic human FOXP3 qPCR Assay. Their in vitro and in vivo potency was confirmed with suppression assay and humanized xenogeneic GVHD (xGVHD) murine model, respectively. Results: LAP+ repurification results in >90% LAP+FOXP3+ Tregs, leaving behind FOXP3- and FOXP3+ nonTregs within the LAP- population. After 4-week expansion, the LAP+ Tregs were >1 billion cells, highly suppressive and anergic in vitro, >90% demethylated in the TSDR and able to maintain TCR Vβ diversity. In the xGVHD model, exogenous CD25-PBMC administered alone results in a median survival of 32 days. The co-transfer of LAP+ Tregs increased median survival to 47 days, while the LAP parent (CD25+) and LAP- nonTregs had median survival of 39 and 31 days, respectively. Conclusions: These preclinical data together provide evidence that LAP+ Tregs are highly purified with fully suppressive function for cell therapy. This population results in a more effective and safer product for immunotherapy to treat GVHD and provides the necessary preclinical data for transition into a clinical trial with LAP+ Tregs to prevent or treat GVHD and other autoimmune diseases." @default.
• W2936705082 created "2019-04-25" @default.
• W2936705082 creator A5007081796 @default.
• W2936705082 creator A5010579045 @default.
• W2936705082 creator A5033076947 @default.
• W2936705082 creator A5037608680 @default.
• W2936705082 creator A5045383722 @default.
• W2936705082 creator A5086473122 @default.
• W2936705082 creator A5091466042 @default.
• W2936705082 date "2019-01-01" @default.
• W2936705082 modified "2023-09-26" @default.
• W2936705082 title "Human LAP⁺GARP⁺FOXP3⁺ regulatory T cells attenuate xenogeneic graft versus host disease" @default.
• W2936705082 cites W1588272261 @default.
• W2936705082 cites W1736404394 @default.
• W2936705082 cites W1785015986 @default.
• W2936705082 cites W1977836556 @default.
• W2936705082 cites W1989354231 @default.
• W2936705082 cites W1990505773 @default.
• W2936705082 cites W1997462243 @default.
• W2936705082 cites W2006392330 @default.
• W2936705082 cites W2009888206 @default.
• W2936705082 cites W2010486962 @default.
• W2936705082 cites W2017230319 @default.
• W2936705082 cites W2026836824 @default.
• W2936705082 cites W2037780312 @default.
• W2936705082 cites W2042073252 @default.
• W2936705082 cites W2045283474 @default.
• W2936705082 cites W2059683433 @default.
• W2936705082 cites W2064282763 @default.
• W2936705082 cites W2065750006 @default.
• W2936705082 cites W2068689362 @default.
• W2936705082 cites W2073701525 @default.
• W2936705082 cites W2085059541 @default.
• W2936705082 cites W2085704445 @default.
• W2936705082 cites W2102202232 @default.
• W2936705082 cites W2106178007 @default.
• W2936705082 cites W2111008821 @default.
• W2936705082 cites W2111329034 @default.
• W2936705082 cites W2113024959 @default.
• W2936705082 cites W2122722718 @default.
• W2936705082 cites W2123013608 @default.
• W2936705082 cites W2138932244 @default.
• W2936705082 cites W2140915979 @default.
• W2936705082 cites W2145353379 @default.
• W2936705082 cites W2149076983 @default.
• W2936705082 cites W2157325100 @default.
• W2936705082 cites W2564152524 @default.
• W2936705082 cites W2737785069 @default.
• W2936705082 cites W2885315731 @default.
• W2936705082 doi "https://doi.org/10.7150/thno.30254" @default.
• W2936705082 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6531299" @default.
• W2936705082 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31149046" @default.
• W2936705082 hasPublicationYear "2019" @default.
• W2936705082 type Work @default.
• W2936705082 sameAs 2936705082 @default.
• W2936705082 citedByCount "10" @default.
• W2936705082 countsByYear W29367050822020 @default.
• W2936705082 countsByYear W29367050822021 @default.
• W2936705082 countsByYear W29367050822022 @default.
• W2936705082 countsByYear W29367050822023 @default.
• W2936705082 crossrefType "journal-article" @default.
• W2936705082 hasAuthorship W2936705082A5007081796 @default.
• W2936705082 hasAuthorship W2936705082A5010579045 @default.
• W2936705082 hasAuthorship W2936705082A5033076947 @default.
• W2936705082 hasAuthorship W2936705082A5037608680 @default.
• W2936705082 hasAuthorship W2936705082A5045383722 @default.
• W2936705082 hasAuthorship W2936705082A5086473122 @default.
• W2936705082 hasAuthorship W2936705082A5091466042 @default.
• W2936705082 hasBestOaLocation W29367050821 @default.
• W2936705082 hasConcept C10882517 @default.
• W2936705082 hasConcept C126322002 @default.
• W2936705082 hasConcept C1491633281 @default.
• W2936705082 hasConcept C150903083 @default.
• W2936705082 hasConcept C185592680 @default.
• W2936705082 hasConcept C203014093 @default.
• W2936705082 hasConcept C207001950 @default.
• W2936705082 hasConcept C2776090121 @default.
• W2936705082 hasConcept C2777701055 @default.
• W2936705082 hasConcept C2778296632 @default.
• W2936705082 hasConcept C2779727006 @default.
• W2936705082 hasConcept C2779972918 @default.
• W2936705082 hasConcept C2908647359 @default.
• W2936705082 hasConcept C2911091166 @default.
• W2936705082 hasConcept C502942594 @default.
• W2936705082 hasConcept C55493867 @default.
• W2936705082 hasConcept C71924100 @default.
• W2936705082 hasConcept C79484868 @default.
• W2936705082 hasConcept C86803240 @default.
• W2936705082 hasConcept C8891405 @default.
• W2936705082 hasConcept C90375314 @default.
• W2936705082 hasConcept C99454951 @default.
• W2936705082 hasConceptScore W2936705082C10882517 @default.
• W2936705082 hasConceptScore W2936705082C126322002 @default.
• W2936705082 hasConceptScore W2936705082C1491633281 @default.
• W2936705082 hasConceptScore W2936705082C150903083 @default.
• W2936705082 hasConceptScore W2936705082C185592680 @default.
• W2936705082 hasConceptScore W2936705082C203014093 @default.
• W2936705082 hasConceptScore W2936705082C207001950 @default.

• next