FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2937092794> ?p ?o ?g. }

• W2937092794 abstract "Cellulose and hemicellulose are the two largest components in lignocellulosic biomass. Enzymes with activities towards cellulose and xylan have attracted great interest in the bioconversion of lignocellulosic biomass, since they have potential in improving the hydrolytic performance and reducing the enzyme costs. Exploring glycoside hydrolases (GHs) with good thermostability and activities on xylan and cellulose would be beneficial to the industrial production of biofuels and bio-based chemicals. A novel GH10 enzyme (XynA) identified from a xylanolytic strain Bacillus sp. KW1 was cloned and expressed. Its optimal pH and temperature were determined to be pH 6.0 and 65 °C. Stability analyses revealed that XynA was stable over a broad pH range (pH 6.0–11.0) after being incubated at 25 °C for 24 h. Moreover, XynA retained over 95% activity after heat treatment at 60 °C for 60 h, and its half-lives at 65 °C and 70 °C were about 12 h and 1.5 h, respectively. More importantly, in terms of substrate specificity, XynA exhibits hydrolytic activities towards xylans, microcrystalline cellulose (filter paper and Avicel), carboxymethyl cellulose (CMC), cellobiose, p-nitrophenyl-β-d-cellobioside (pNPC), and p-nitrophenyl-β-d-glucopyranoside (pNPG). Furthermore, the addition of XynA into commercial cellulase in the hydrolysis of pretreated corn stover resulted in remarkable increases (the relative increases may up to 90%) in the release of reducing sugars. Finally, it is worth mentioning that XynA only shows high amino acid sequence identity (88%) with rXynAHJ14, a GH10 xylanase with no activity on CMC. The similarities with other characterized GH10 enzymes, including xylanases and bifunctional xylanase/cellulase enzymes, are no more than 30%. XynA is a novel thermostable GH10 xylanase with a wide substrate spectrum. It displays good stability in a broad range of pH and high temperatures, and exhibits activities towards xylans and a wide variety of cellulosic substrates, which are not found in other GH10 enzymes. The enzyme also has high capacity in saccharification of pretreated corn stover. These characteristics make XynA a good candidate not only for assisting cellulase in lignocellulosic biomass hydrolysis, but also for the research on structure–function relationship of bifunctional xylanase/cellulase." @default.
• W2937092794 created "2019-04-25" @default.
• W2937092794 creator A5002657998 @default.
• W2937092794 creator A5016414843 @default.
• W2937092794 creator A5055174666 @default.
• W2937092794 creator A5061137450 @default.
• W2937092794 creator A5065811267 @default.
• W2937092794 creator A5086096434 @default.
• W2937092794 creator A5091762266 @default.
• W2937092794 date "2019-03-09" @default.
• W2937092794 modified "2023-10-14" @default.
• W2937092794 title "A novel thermostable GH10 xylanase with activities on a wide variety of cellulosic substrates from a xylanolytic Bacillus strain exhibiting significant synergy with commercial Celluclast 1.5 L in pretreated corn stover hydrolysis" @default.
• W2937092794 cites W1605341148 @default.
• W2937092794 cites W1880885864 @default.
• W2937092794 cites W1926343342 @default.
• W2937092794 cites W1965195162 @default.
• W2937092794 cites W1975217396 @default.
• W2937092794 cites W1975883889 @default.
• W2937092794 cites W1978349736 @default.
• W2937092794 cites W1990994271 @default.
• W2937092794 cites W2017053172 @default.
• W2937092794 cites W2018154056 @default.
• W2937092794 cites W2019545699 @default.
• W2937092794 cites W2019650510 @default.
• W2937092794 cites W2029082881 @default.
• W2937092794 cites W2030321174 @default.
• W2937092794 cites W2044194502 @default.
• W2937092794 cites W2054796509 @default.
• W2937092794 cites W2056937018 @default.
• W2937092794 cites W2057716319 @default.
• W2937092794 cites W2066573910 @default.
• W2937092794 cites W2067659396 @default.
• W2937092794 cites W2088282660 @default.
• W2937092794 cites W2088299609 @default.
• W2937092794 cites W2122559203 @default.
• W2937092794 cites W2133604239 @default.
• W2937092794 cites W2134567163 @default.
• W2937092794 cites W2135123110 @default.
• W2937092794 cites W2137368412 @default.
• W2937092794 cites W2166989211 @default.
• W2937092794 cites W2323306453 @default.
• W2937092794 cites W2513817655 @default.
• W2937092794 cites W2516767128 @default.
• W2937092794 cites W2526191639 @default.
• W2937092794 cites W2761123924 @default.
• W2937092794 cites W2769002467 @default.
• W2937092794 cites W2771266516 @default.
• W2937092794 cites W2775571341 @default.
• W2937092794 cites W2788084066 @default.
• W2937092794 cites W2790183111 @default.
• W2937092794 cites W2791723139 @default.
• W2937092794 cites W2805689178 @default.
• W2937092794 cites W2811046634 @default.
• W2937092794 cites W2883781359 @default.
• W2937092794 cites W2888381752 @default.
• W2937092794 cites W4232534952 @default.
• W2937092794 doi "https://doi.org/10.1186/s13068-019-1389-8" @default.
• W2937092794 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6408826" @default.
• W2937092794 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30899328" @default.
• W2937092794 hasPublicationYear "2019" @default.
• W2937092794 type Work @default.
• W2937092794 sameAs 2937092794 @default.
• W2937092794 citedByCount "43" @default.
• W2937092794 countsByYear W29370927942019 @default.
• W2937092794 countsByYear W29370927942020 @default.
• W2937092794 countsByYear W29370927942021 @default.
• W2937092794 countsByYear W29370927942022 @default.
• W2937092794 countsByYear W29370927942023 @default.
• W2937092794 crossrefType "journal-article" @default.
• W2937092794 hasAuthorship W2937092794A5002657998 @default.
• W2937092794 hasAuthorship W2937092794A5016414843 @default.
• W2937092794 hasAuthorship W2937092794A5055174666 @default.
• W2937092794 hasAuthorship W2937092794A5061137450 @default.
• W2937092794 hasAuthorship W2937092794A5065811267 @default.
• W2937092794 hasAuthorship W2937092794A5086096434 @default.
• W2937092794 hasAuthorship W2937092794A5091762266 @default.
• W2937092794 hasBestOaLocation W29370927941 @default.
• W2937092794 hasConcept C100544194 @default.
• W2937092794 hasConcept C116174643 @default.
• W2937092794 hasConcept C141603559 @default.
• W2937092794 hasConcept C163283067 @default.
• W2937092794 hasConcept C178790620 @default.
• W2937092794 hasConcept C181199279 @default.
• W2937092794 hasConcept C185592680 @default.
• W2937092794 hasConcept C2775859485 @default.
• W2937092794 hasConcept C2776073808 @default.
• W2937092794 hasConcept C2776081644 @default.
• W2937092794 hasConcept C2776726243 @default.
• W2937092794 hasConcept C2778234585 @default.
• W2937092794 hasConcept C2779251873 @default.
• W2937092794 hasConcept C2779920640 @default.
• W2937092794 hasConcept C2780074830 @default.
• W2937092794 hasConcept C2780412358 @default.
• W2937092794 hasConcept C2781216036 @default.
• W2937092794 hasConcept C31903555 @default.
• W2937092794 hasConcept C537181965 @default.
• W2937092794 hasConcept C55493867 @default.
• W2937092794 hasConcept C94412978 @default.
• W2937092794 hasConceptScore W2937092794C100544194 @default.
• W2937092794 hasConceptScore W2937092794C116174643 @default.

• next