FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2937492780> ?p ?o ?g. }

• W2937492780 abstract "Background Anti-nuclear antibody (ANA) positivity is a principal feature of individuals with an autoimmune disease, yet up to one in five healthy individuals are ANA-positive (ANA+) and will never develop overt disease. Understanding differences in immune cell physiology between ANA+healthy individuals and individuals with clinical SLE remains a critical goal in the understanding of SLE pathogenesis across ethnicities. Methods Blood specimens and information on disease activity were collected from European (EA) and African American (AA) individuals classified and matched in groups as ANA- healthy controls (n=24), ANA +healthy (n=24) or SLE patients (n=24). Single-cell analysis of cell surface markers was completed by mass cytometry on PBMCs and cellular heterogeneity was visualized using tSNE (figure 1A–B) and manual gating. Further, phospho-specific flow cytometry was used to measure basal levels of pERK, pPLCg2 and p38 and expression following CD3/CD28 (TCR) and anti-IgG and IgM (BCR) stimulation. Whole genome RNA-sequencing was performed on flow cytometry sorted T cells, B cells and monocytes from 35 matched ANA-, ANA +and SLE patients followed by weighted correlation network analysis (WGCNA) and pathway enrichment analyses. Results Both European and African American SLE patients were distinguished from healthy individuals by T cell proliferation (p=0.002) (figure 1C), plasmacytoid dendritic cell activation (p=0.021) and elevated stem cell factor (p=0.0003). EA ANA+healthy individuals exhibited greater immune regulation with reduced T cell numbers (p=0.002) (figure 1C), decreased activation of dendritic cells (p=0.039) and transitional B cells (0.033), and elevated expression of the inhibitory receptor CD85j (p=0.042) on specific immune cell subsets compared to ANA- healthy subjects. Further, a module associated with hematopoiesis, T cell activation and intrinsic apoptosis signaling pathways is expressed at a higher level in T cells of EA ANA+individuals. In contrast, AA ANA+healthy individuals had elevated plasma levels of IL-6 (p=0.018) and reduced inhibitory receptor expression (p=0.0089) compared to ANA- healthy controls. Gene expression modules associated with viral responses and type I IFN pathway activation were identified in AA SLE patient B cells, while similar expression modules were only found in the monocytes of European American SLE patients. Conclusions These results highlight the importance of stem cell factor and T cell expansion in SLE pathogenesis, and suggest that mechanisms of SLE pathogenesis differ by ethnicity. ANA +European Americans may have more effective regulatory mechanisms in place to prevent transition to classified autoimmune disease. Funding Source(s): This work was supported by the NIH under award numbers U54GM104938, U01AI101934, U19AI082714, and P30AR053483." @default.
• W2937492780 created "2019-04-25" @default.
• W2937492780 creator A5000883278 @default.
• W2937492780 creator A5001083935 @default.
• W2937492780 creator A5010973711 @default.
• W2937492780 creator A5014019068 @default.
• W2937492780 creator A5028844073 @default.
• W2937492780 creator A5031271622 @default.
• W2937492780 creator A5032779152 @default.
• W2937492780 creator A5052053321 @default.
• W2937492780 creator A5055486497 @default.
• W2937492780 creator A5057418379 @default.
• W2937492780 creator A5065514371 @default.
• W2937492780 creator A5068399737 @default.
• W2937492780 creator A5078244267 @default.
• W2937492780 creator A5088206527 @default.
• W2937492780 date "2019-04-01" @default.
• W2937492780 modified "2023-09-23" @default.
• W2937492780 title "233 Autoantibody-positive healthy individuals constrain T cell pathways to regulate autoimmune disease" @default.
• W2937492780 doi "https://doi.org/10.1136/lupus-2019-lsm.233" @default.
• W2937492780 hasPublicationYear "2019" @default.
• W2937492780 type Work @default.
• W2937492780 sameAs 2937492780 @default.
• W2937492780 citedByCount "0" @default.
• W2937492780 crossrefType "proceedings-article" @default.
• W2937492780 hasAuthorship W2937492780A5000883278 @default.
• W2937492780 hasAuthorship W2937492780A5001083935 @default.
• W2937492780 hasAuthorship W2937492780A5010973711 @default.
• W2937492780 hasAuthorship W2937492780A5014019068 @default.
• W2937492780 hasAuthorship W2937492780A5028844073 @default.
• W2937492780 hasAuthorship W2937492780A5031271622 @default.
• W2937492780 hasAuthorship W2937492780A5032779152 @default.
• W2937492780 hasAuthorship W2937492780A5052053321 @default.
• W2937492780 hasAuthorship W2937492780A5055486497 @default.
• W2937492780 hasAuthorship W2937492780A5057418379 @default.
• W2937492780 hasAuthorship W2937492780A5065514371 @default.
• W2937492780 hasAuthorship W2937492780A5068399737 @default.
• W2937492780 hasAuthorship W2937492780A5078244267 @default.
• W2937492780 hasAuthorship W2937492780A5088206527 @default.
• W2937492780 hasBestOaLocation W29374927801 @default.
• W2937492780 hasConcept C159654299 @default.
• W2937492780 hasConcept C163764329 @default.
• W2937492780 hasConcept C203014093 @default.
• W2937492780 hasConcept C2776090121 @default.
• W2937492780 hasConcept C2780130043 @default.
• W2937492780 hasConcept C553184892 @default.
• W2937492780 hasConcept C71924100 @default.
• W2937492780 hasConcept C8891405 @default.
• W2937492780 hasConceptScore W2937492780C159654299 @default.
• W2937492780 hasConceptScore W2937492780C163764329 @default.
• W2937492780 hasConceptScore W2937492780C203014093 @default.
• W2937492780 hasConceptScore W2937492780C2776090121 @default.
• W2937492780 hasConceptScore W2937492780C2780130043 @default.
• W2937492780 hasConceptScore W2937492780C553184892 @default.
• W2937492780 hasConceptScore W2937492780C71924100 @default.
• W2937492780 hasConceptScore W2937492780C8891405 @default.
• W2937492780 hasLocation W29374927801 @default.
• W2937492780 hasOpenAccess W2937492780 @default.
• W2937492780 hasPrimaryLocation W29374927801 @default.
• W2937492780 hasRelatedWork W1965561010 @default.
• W2937492780 hasRelatedWork W2013215544 @default.
• W2937492780 hasRelatedWork W2025289327 @default.
• W2937492780 hasRelatedWork W2036922016 @default.
• W2937492780 hasRelatedWork W2158432938 @default.
• W2937492780 hasRelatedWork W2187276195 @default.
• W2937492780 hasRelatedWork W2203085604 @default.
• W2937492780 hasRelatedWork W2232935379 @default.
• W2937492780 hasRelatedWork W2316420891 @default.
• W2937492780 hasRelatedWork W2354116007 @default.
• W2937492780 hasRelatedWork W2368777596 @default.
• W2937492780 hasRelatedWork W2414689907 @default.
• W2937492780 hasRelatedWork W2416762763 @default.
• W2937492780 hasRelatedWork W2429252590 @default.
• W2937492780 hasRelatedWork W2649051505 @default.
• W2937492780 hasRelatedWork W2899287906 @default.
• W2937492780 hasRelatedWork W2902514337 @default.
• W2937492780 hasRelatedWork W2943035441 @default.
• W2937492780 hasRelatedWork W3085592645 @default.
• W2937492780 hasRelatedWork W3094527185 @default.
• W2937492780 isParatext "false" @default.
• W2937492780 isRetracted "false" @default.
• W2937492780 magId "2937492780" @default.
• W2937492780 workType "article" @default.