FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2937680384> ?p ?o ?g. }

• W2937680384 abstract "Alphaviruses and flaviviruses are important human pathogens that include Chikungunya, Dengue and Zika virus, which can cause diseases in humans ranging from arthralgia to hemorrhagic fevers and microcephaly. It was previously shown that treatment with Surface (S) layer protein, present on the bacterial cell-envelope of Lactobacillus acidophilus, is able to inhibit viral and bacterial infections by blocking the pathogen’s interaction with DC-SIGN, a trans-membrane protein that is a C-type calcium-dependent lectin. DC-SIGN is known to act an attachment factor for several viruses including alphaviruses and flaviviruses. In the present work we used alphaviruses and flaviviruses as a model system to dissect the mechanism of S-layer inhibition. To evaluate the protective effect of S-layer in DC-SIGN-expressing cells, we used 3T3 cells, either WT or stably expressing DC-SIGN, and we tested the alphaviruses Semliki Forest virus and Chikungunya virus and the flaviviruses Zika virus and Dengue virus. DC-SIGN expression significantly enhanced infection by all four viruses. Treatment of the cells with S-layer prior to infection decreased virus infectivity only in cells expressing DC-SIGN. Using confocal microscopy and flow cytometry we showed that S-layer binding to the cells was independent of DC-SIGN expression. However, S-protein only prevented Semliki Forest virus adsorption and internalization in DC-SIGN-expressing cells and had no effect on virus binding to DC-SIGN-negative cells. Inhibition of virus binding occurred in a time dependent manner, with a significant reduction of infection requiring at least a 30 minute pre-incubation of S-layer with DC-SIGN-expressing cells. These results suggest that S-layer has a different mechanism of action compared to mannan, a common DC-sign binding compound that has an immediate effect in blocking viral infection. This difference could reflect slower kinetics of S-layer binding to the DC-sign present at the plasma membrane. Alternatively, the S-layer/DC-SIGN interaction may trigger the activation of signaling pathways that are required for the inhibition of viral infection. Together our results add important information relevant to the potential use of Lactobacillus acidophilus as an antiviral therapy." @default.
• W2937680384 created "2019-04-25" @default.
• W2937680384 creator A5005406597 @default.
• W2937680384 creator A5035008126 @default.
• W2937680384 creator A5062757242 @default.
• W2937680384 creator A5069721989 @default.
• W2937680384 creator A5076442194 @default.
• W2937680384 creator A5078357217 @default.
• W2937680384 date "2019-04-16" @default.
• W2937680384 modified "2023-10-17" @default.
• W2937680384 title "Surface (S) Layer Proteins of Lactobacillus acidophilus Block Virus Infection via DC-SIGN Interaction" @default.
• W2937680384 cites W118714628 @default.
• W2937680384 cites W1517253107 @default.
• W2937680384 cites W1585798840 @default.
• W2937680384 cites W1829239725 @default.
• W2937680384 cites W1857453373 @default.
• W2937680384 cites W1962551363 @default.
• W2937680384 cites W1965326485 @default.
• W2937680384 cites W1986002187 @default.
• W2937680384 cites W1988176421 @default.
• W2937680384 cites W2000562335 @default.
• W2937680384 cites W2001847828 @default.
• W2937680384 cites W2009381049 @default.
• W2937680384 cites W2013947028 @default.
• W2937680384 cites W2020016349 @default.
• W2937680384 cites W2033370704 @default.
• W2937680384 cites W2055606591 @default.
• W2937680384 cites W2075551764 @default.
• W2937680384 cites W2090210049 @default.
• W2937680384 cites W2090973041 @default.
• W2937680384 cites W2099332898 @default.
• W2937680384 cites W2111564229 @default.
• W2937680384 cites W2126040408 @default.
• W2937680384 cites W2129393743 @default.
• W2937680384 cites W2131484195 @default.
• W2937680384 cites W2132117914 @default.
• W2937680384 cites W2150120689 @default.
• W2937680384 cites W2161976205 @default.
• W2937680384 cites W2167871325 @default.
• W2937680384 cites W2169109442 @default.
• W2937680384 cites W2277153172 @default.
• W2937680384 cites W2481252765 @default.
• W2937680384 cites W2486155819 @default.
• W2937680384 cites W2534202047 @default.
• W2937680384 cites W2563306892 @default.
• W2937680384 cites W2612605262 @default.
• W2937680384 cites W2790123612 @default.
• W2937680384 cites W2988949874 @default.
• W2937680384 cites W4238126834 @default.
• W2937680384 doi "https://doi.org/10.3389/fmicb.2019.00810" @default.
• W2937680384 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6477042" @default.
• W2937680384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31040840" @default.
• W2937680384 hasPublicationYear "2019" @default.
• W2937680384 type Work @default.
• W2937680384 sameAs 2937680384 @default.
• W2937680384 citedByCount "32" @default.
• W2937680384 countsByYear W29376803842019 @default.
• W2937680384 countsByYear W29376803842020 @default.
• W2937680384 countsByYear W29376803842021 @default.
• W2937680384 countsByYear W29376803842022 @default.
• W2937680384 countsByYear W29376803842023 @default.
• W2937680384 crossrefType "journal-article" @default.
• W2937680384 hasAuthorship W2937680384A5005406597 @default.
• W2937680384 hasAuthorship W2937680384A5035008126 @default.
• W2937680384 hasAuthorship W2937680384A5062757242 @default.
• W2937680384 hasAuthorship W2937680384A5069721989 @default.
• W2937680384 hasAuthorship W2937680384A5076442194 @default.
• W2937680384 hasAuthorship W2937680384A5078357217 @default.
• W2937680384 hasBestOaLocation W29376803841 @default.
• W2937680384 hasConcept C100544194 @default.
• W2937680384 hasConcept C104317684 @default.
• W2937680384 hasConcept C106358424 @default.
• W2937680384 hasConcept C140704245 @default.
• W2937680384 hasConcept C159047783 @default.
• W2937680384 hasConcept C196594336 @default.
• W2937680384 hasConcept C2522874641 @default.
• W2937680384 hasConcept C2776651480 @default.
• W2937680384 hasConcept C2777062976 @default.
• W2937680384 hasConcept C2777474056 @default.
• W2937680384 hasConcept C2779635636 @default.
• W2937680384 hasConcept C2780206646 @default.
• W2937680384 hasConcept C2780568436 @default.
• W2937680384 hasConcept C2780963514 @default.
• W2937680384 hasConcept C2781273456 @default.
• W2937680384 hasConcept C533803919 @default.
• W2937680384 hasConcept C55493867 @default.
• W2937680384 hasConcept C67705224 @default.
• W2937680384 hasConcept C86803240 @default.
• W2937680384 hasConcept C89138240 @default.
• W2937680384 hasConcept C89423630 @default.
• W2937680384 hasConceptScore W2937680384C100544194 @default.
• W2937680384 hasConceptScore W2937680384C104317684 @default.
• W2937680384 hasConceptScore W2937680384C106358424 @default.
• W2937680384 hasConceptScore W2937680384C140704245 @default.
• W2937680384 hasConceptScore W2937680384C159047783 @default.
• W2937680384 hasConceptScore W2937680384C196594336 @default.
• W2937680384 hasConceptScore W2937680384C2522874641 @default.
• W2937680384 hasConceptScore W2937680384C2776651480 @default.
• W2937680384 hasConceptScore W2937680384C2777062976 @default.
• W2937680384 hasConceptScore W2937680384C2777474056 @default.

• next