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- W2937902330 abstract "β-2-himachalen-6-ol (HC) is a major sesquiterpene that was isolated from theLebanese Daucus carota ssp. carota and was shown to possess potent anticancer activityagainst various cancer cell lines. The aim of the present study was to explore the in vitroand in vivo anticancer activities of HC and to investigate the possible mechanisms ofaction involved.Lebanese Daucus carota spp. carota plant oil was extracted from dried matureumbels with methanol/acetone (1/1) and HC was purified using silica gel columnchromatography to 92% purity as confirmed by GC-MS and NMR.The in vitro effect of HC on cell viability was tested against a panel of coloncancer cell lines (Caco-2, HT-29, SK-Co, LoVo, SW-1116 and T-84), a normal colon cellline CCD-33Co, the HaCaT-ras II-4 epidermal squamous carcinoma and the 4T1 breastcancer cell lines. HC displayed potent cytotoxic activity as measured by the WST-1 cellproliferation assay against all cancer cell lines with an IC50 ranging between 8-14.5μg/mland an IC90 between 19-36μg/ml. No significant cytotoxic effect was observed on thenormal colon cell line at the concentrations used.Upon treatment with HC, flow cytometry and western blot analyses showedsimilar results for both the SW1116 and HaCaT-ras II-4 cell lines, with an observedaccumulation of cells in the sub-G1 apoptotic phase and decreased cell population in Sand G2/M phases indicating cell cycle arrest at sub-G1phase. A decrease in the p-Erk, p-Akt, anti-apoptotic protein (Bcl-2) and an increase in pro-apoptotic proteins (p53, Bax,p21, cleaved Caspase-3 and PARP) was also observed.The in vivo anticancer effect of HC was investigated using DMH-induced colon,DMBA/TPA-induced skin carcinogenesis and 4T1 breast metastatic murine models ofcancer. HC proved to be effective in reducing cancer incidence and size in all theexperiments with a relatively low hepato-toxicity.The present study established that HC is a potent and relatively safe anticancercompound that induces apoptosis through the inhibition of the MAPK/Erk and PI3K/ Aktpathways." @default.
- W2937902330 created "2019-04-25" @default.
- W2937902330 creator A5030504899 @default.
- W2937902330 date "2019-02-28" @default.
- W2937902330 modified "2023-09-27" @default.
- W2937902330 title "The in vitro and in vivo anticancer effect of β-2-himachalen-6-ol isolated from the Lebanese Daucus carota spp. carota." @default.
- W2937902330 doi "https://doi.org/10.15126/thesis.00850381" @default.
- W2937902330 hasPublicationYear "2019" @default.
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