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- W2938202062 endingPage "106146" @default.
- W2938202062 startingPage "106146" @default.
- W2938202062 abstract "Review and analyses of the experimental data indicate that in nearly all cases bending elastic constants of the effective springs between bp of DNA actually undergo a net increase with increasing T from 278 to 315 K. The exceptions to this rule are bending elastic constants obtained from equilibrium topoisomer distributions of a 2686 bp pUC19 DNA by assuming a fixed T-independent value of the torsion elastic constant. When the same data are analyzed using measured T-dependent values of the torsion elastic constant, which decline with increasing T, a modest increase in bending elastic constant with increasing T is obtained. After revising the torsion elastic constants of the previously formulated two-state cooperative transition model to account for additional data, that model is fitted to the bending elastic constants reckoned from the aforementioned topoisomer distributions to determine the best-fit values for each state. The rather good fit implies a strong negative linear correlation between the inverse bending and inverse torsion elastic constants as T is varied. Predictions of the resulting two-state model, wherein each state has fixed bending and torsion elastic constants, agree surprisingly well with single-molecule relative extension and torque data. The same model also yields good agreement with numerous other experimental data. With increasing T the equilibrium is shifted from the (longer, torsionally stiffer, flexurally softer) b-state toward the (shorter, torsionally softer, flexurally stiffer) a-state. This transition is suggested to be the origin of the so-called broad pre-melting transition exhibited by many, but not all, DNAs." @default.
- W2938202062 created "2019-04-25" @default.
- W2938202062 creator A5070015753 @default.
- W2938202062 date "2019-08-01" @default.
- W2938202062 modified "2023-09-26" @default.
- W2938202062 title "Temperature-dependence of the bending elastic constant of DNA and extension of the two-state model. Tests and new insights" @default.
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- W2938202062 doi "https://doi.org/10.1016/j.bpc.2019.106146" @default.
- W2938202062 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31158653" @default.
- W2938202062 hasPublicationYear "2019" @default.
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