FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2938241454> ?p ?o ?g. }

• W2938241454 endingPage "89" @default.
• W2938241454 startingPage "80" @default.
• W2938241454 abstract "RNA interference (RNAi) refers to the process of suppression of gene expression in eukaryotes, which has a great potential for the control of pest and diseases. Unfortunately, the efficacy of this technology is limited or at best variable in some insects. In the African sweet potato weevil (SPW) Cylas puncticollis, a devastating pest that affects the sweet potato production in Sub-Saharan Africa (SSA), the RNAi response was highly efficient when dsRNA was delivered by injection, but it showed a reduced response by oral feeding. In the present study, the role of nucleases in the reduced RNAi efficiency in C. puncticollis is investigated. Several putative dsRNases were first identified in the transcriptome of the SPW through homology search and were subsequently further characterized. Two of these dsRNases were specifically expressed in the gut tissue of the insect and we could demonstrate through RNAi experiments that these affected dsRNA stability in the gut. Furthermore, RNAi-of-RNAi studies, using snf7 as a reporter gene, demonstrated that silencing one of these nucleases, Cp-dsRNase-3, clearly increases RNAi efficacy. After silencing this nuclease, significantly higher mortality was observed in dssnf7-treated insects 14 days post-feeding as compared to control treatments, and the gene downregulation was confirmed at the transcript level via qPCR analysis. Taken together, our results demonstrate that the RNAi efficiency is certainly impaired by nuclease activity in the gut environment of the SPW Cylas puncticollis." @default.
• W2938241454 created "2019-04-25" @default.
• W2938241454 creator A5001487967 @default.
• W2938241454 creator A5020649355 @default.
• W2938241454 creator A5066515722 @default.
• W2938241454 creator A5071131695 @default.
• W2938241454 date "2019-07-01" @default.
• W2938241454 modified "2023-10-16" @default.
• W2938241454 title "Nuclease activity decreases the RNAi response in the sweetpotato weevil Cylas puncticollis" @default.
• W2938241454 cites W1138768165 @default.
• W2938241454 cites W1509976803 @default.
• W2938241454 cites W1577577364 @default.
• W2938241454 cites W1968992967 @default.
• W2938241454 cites W1973498157 @default.
• W2938241454 cites W1975763913 @default.
• W2938241454 cites W1978290762 @default.
• W2938241454 cites W1978425510 @default.
• W2938241454 cites W1984104677 @default.
• W2938241454 cites W1990027679 @default.
• W2938241454 cites W1992170226 @default.
• W2938241454 cites W2012656947 @default.
• W2938241454 cites W2020051355 @default.
• W2938241454 cites W2023690567 @default.
• W2938241454 cites W2035471199 @default.
• W2938241454 cites W2037616695 @default.
• W2938241454 cites W2042657797 @default.
• W2938241454 cites W2051736425 @default.
• W2938241454 cites W2054363760 @default.
• W2938241454 cites W2062046551 @default.
• W2938241454 cites W2063895123 @default.
• W2938241454 cites W2072994220 @default.
• W2938241454 cites W2079887141 @default.
• W2938241454 cites W2086244179 @default.
• W2938241454 cites W2090047954 @default.
• W2938241454 cites W2092704486 @default.
• W2938241454 cites W2103161297 @default.
• W2938241454 cites W2107277218 @default.
• W2938241454 cites W2115933717 @default.
• W2938241454 cites W2124791602 @default.
• W2938241454 cites W2127229134 @default.
• W2938241454 cites W2128412621 @default.
• W2938241454 cites W2138581811 @default.
• W2938241454 cites W2142484720 @default.
• W2938241454 cites W2143427287 @default.
• W2938241454 cites W2157530253 @default.
• W2938241454 cites W2159448158 @default.
• W2938241454 cites W2274250464 @default.
• W2938241454 cites W2311203695 @default.
• W2938241454 cites W2411345191 @default.
• W2938241454 cites W2442283999 @default.
• W2938241454 cites W2500422463 @default.
• W2938241454 cites W2501505851 @default.
• W2938241454 cites W2554569574 @default.
• W2938241454 cites W2565175892 @default.
• W2938241454 cites W2578910048 @default.
• W2938241454 cites W2617694374 @default.
• W2938241454 cites W2771785548 @default.
• W2938241454 cites W2789971483 @default.
• W2938241454 cites W2795728717 @default.
• W2938241454 cites W2803644409 @default.
• W2938241454 doi "https://doi.org/10.1016/j.ibmb.2019.04.001" @default.
• W2938241454 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31009678" @default.
• W2938241454 hasPublicationYear "2019" @default.
• W2938241454 type Work @default.
• W2938241454 sameAs 2938241454 @default.
• W2938241454 citedByCount "51" @default.
• W2938241454 countsByYear W29382414542019 @default.
• W2938241454 countsByYear W29382414542020 @default.
• W2938241454 countsByYear W29382414542021 @default.
• W2938241454 countsByYear W29382414542022 @default.
• W2938241454 countsByYear W29382414542023 @default.
• W2938241454 crossrefType "journal-article" @default.
• W2938241454 hasAuthorship W2938241454A5001487967 @default.
• W2938241454 hasAuthorship W2938241454A5020649355 @default.
• W2938241454 hasAuthorship W2938241454A5066515722 @default.
• W2938241454 hasAuthorship W2938241454A5071131695 @default.
• W2938241454 hasConcept C104317684 @default.
• W2938241454 hasConcept C119056186 @default.
• W2938241454 hasConcept C150194340 @default.
• W2938241454 hasConcept C162317418 @default.
• W2938241454 hasConcept C166703698 @default.
• W2938241454 hasConcept C2191507 @default.
• W2938241454 hasConcept C2777271071 @default.
• W2938241454 hasConcept C54355233 @default.
• W2938241454 hasConcept C67705224 @default.
• W2938241454 hasConcept C86803240 @default.
• W2938241454 hasConcept C95444343 @default.
• W2938241454 hasConceptScore W2938241454C104317684 @default.
• W2938241454 hasConceptScore W2938241454C119056186 @default.
• W2938241454 hasConceptScore W2938241454C150194340 @default.
• W2938241454 hasConceptScore W2938241454C162317418 @default.
• W2938241454 hasConceptScore W2938241454C166703698 @default.
• W2938241454 hasConceptScore W2938241454C2191507 @default.
• W2938241454 hasConceptScore W2938241454C2777271071 @default.
• W2938241454 hasConceptScore W2938241454C54355233 @default.
• W2938241454 hasConceptScore W2938241454C67705224 @default.
• W2938241454 hasConceptScore W2938241454C86803240 @default.
• W2938241454 hasConceptScore W2938241454C95444343 @default.

• next