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- W2938262806 abstract "Aminolevulinic acid based photodynamic therapy (ALA-PDT), in which protoporphyrin IX (PpIX) induces target-specific cell death in the presence of light and oxygen, is an effective treatment for actinic keratosis (AK). However, standard PDT can elicit stinging pain during illumination, a major drawback for patients. In a new regimen called metronomic PDT (mPDT), blue light illumination is delivered in the clinic concurrently with ALA application, rather than after a 1-hour pre-incubation (conventional regimen), providing a painless experience that is nearly as effective as conventional PDT for AK lesion clearance. In this investigation, a murine AK model (generated by repeated UVB exposure of Skh-1 mice) was treated with either mPDT (ALA + immediate illumination, 405 nm for 60 minutes) or PDT (1-hour incubation with ALA + illumination, 405 nm for 8.25 minutes). Lesion clearance was followed by area measurement, and samples were harvested for mechanistic analyses. Compared to pretreatment (100%), the average lesion area was reduced to 47% and 32% in PDT, and to 57% and 40% in mPDT at 1 and 2 weeks post PDT, respectively. Relative to untreated controls, enhanced cell death (histomorphology by H&E staining and apoptosis by TUNEL assay), generation of Reactive Oxygen Species (ROS; CM-H2DCFDA staining) and autophagy (Atg 5 and Atg 7 expression) were observed in both PDT and mPDT samples. Activation of cleaved Caspase-3 was specifically observed only in PDT samples. Immunomodulation by inflammatory cells was observed by enhanced infiltration/retention of neutrophils and macrophages in metronomic PDT samples. Our results suggest that metronomic PDT can not only be painless but also be as effective as conventional PDT for treatment of AK, while the mechanisms may be quite different." @default.
- W2938262806 created "2019-04-25" @default.
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- W2938262806 date "2019-05-01" @default.
- W2938262806 modified "2023-09-30" @default.
- W2938262806 title "749 Comparison of cell death and immune response in painless (metronomic) versus conventional photodynamic therapy for treatment of actinic keratosis in a murine model" @default.
- W2938262806 doi "https://doi.org/10.1016/j.jid.2019.03.825" @default.
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