Matches in SemOpenAlex for { <https://semopenalex.org/work/W2938597352> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2938597352 abstract "Ferric iron (Fe3+) can be used as a terminal electron acceptor by iron-reducing microorganisms to facilitate cellular respiration under anoxic conditions. In contrast, ferrous iron (Fe2+) is used as an electron donor by iron-oxidising microorganisms. The presence of dissimilatory pathways in iron metabolisers maintain bioavailable iron in the environment for other organisms. Redox reactions between gram-negative bacteria and iron usually occur through an extracellular electron transport (EET) pathway that allows electrons to cross from the inner membrane to the extracellular environment or vice versa. The study of the redox pathway has been thoroughly studied in the iron reducer Shewanella oneidensis, however, there is little information about the metabolic pathways used by other iron metabolisers. In this thesis, the environmental isolates Acinetobacter and Citrobacter have been described as novel iron reducing bacteria by using a combination of techniques including ferrozine assays, cytochrome identification methods and transcriptomic analysis. Results in this thesis suggest that Acinetobacter (previously described as a strict aerobic microorganism) in fact is capable of respiring using iron when oxygen is not available. In contrast, Citrobacter’s iron reduction pathway seems to involve fermentative processes. These results point out that extracellular electron transport is not the only mechanism in dissimilatory iron metabolism. Moreover, these results suggest that anaerobic environments could be a reservoir for pathogenic strains of Acinetobacter, as it has been shown that this species do not require oxygen to survive. In addition to the study of new iron reducers, an overexpression system has been developed to study proteins involved in metal oxidation pathways without the need of culturing the notoriously slow-growing iron oxidising bacteria. The genes cyc2 and cyc2PV-1, which encode outer membranes cytochromes from Acidithiobacillus ferrooxidans and Mariprofundus ferrooxydans respectively, have been transformed into the iron reducer Shewanella oneidensis for protein expression optimization. The optimization of this process offers great possibility for the future study and applications of metal oxidation pathways." @default.
- W2938597352 created "2019-04-25" @default.
- W2938597352 creator A5027277605 @default.
- W2938597352 date "2018-09-01" @default.
- W2938597352 modified "2023-09-23" @default.
- W2938597352 title "Alternative electron transfer pathways in iron-metabolising bacteria" @default.
- W2938597352 cites W1554068896 @default.
- W2938597352 cites W1769492880 @default.
- W2938597352 cites W1989421019 @default.
- W2938597352 cites W2028717873 @default.
- W2938597352 cites W2034688177 @default.
- W2938597352 cites W2035079086 @default.
- W2938597352 cites W2051460146 @default.
- W2938597352 cites W2102877662 @default.
- W2938597352 cites W2128950579 @default.
- W2938597352 cites W2134065242 @default.
- W2938597352 cites W2148749381 @default.
- W2938597352 cites W2171510989 @default.
- W2938597352 cites W2267069928 @default.
- W2938597352 cites W2474685832 @default.
- W2938597352 cites W2903975151 @default.
- W2938597352 hasPublicationYear "2018" @default.
- W2938597352 type Work @default.
- W2938597352 sameAs 2938597352 @default.
- W2938597352 citedByCount "0" @default.
- W2938597352 crossrefType "dissertation" @default.
- W2938597352 hasAuthorship W2938597352A5027277605 @default.
- W2938597352 hasConcept C123669783 @default.
- W2938597352 hasConcept C142520446 @default.
- W2938597352 hasConcept C175605896 @default.
- W2938597352 hasConcept C178790620 @default.
- W2938597352 hasConcept C179104552 @default.
- W2938597352 hasConcept C181199279 @default.
- W2938597352 hasConcept C185592680 @default.
- W2938597352 hasConcept C192989942 @default.
- W2938597352 hasConcept C24840226 @default.
- W2938597352 hasConcept C2775832776 @default.
- W2938597352 hasConcept C2776217839 @default.
- W2938597352 hasConcept C2778824058 @default.
- W2938597352 hasConcept C2781170380 @default.
- W2938597352 hasConcept C2900893 @default.
- W2938597352 hasConcept C48843007 @default.
- W2938597352 hasConcept C523546767 @default.
- W2938597352 hasConcept C54355233 @default.
- W2938597352 hasConcept C55493867 @default.
- W2938597352 hasConcept C55904794 @default.
- W2938597352 hasConcept C62231903 @default.
- W2938597352 hasConcept C75473681 @default.
- W2938597352 hasConcept C86803240 @default.
- W2938597352 hasConceptScore W2938597352C123669783 @default.
- W2938597352 hasConceptScore W2938597352C142520446 @default.
- W2938597352 hasConceptScore W2938597352C175605896 @default.
- W2938597352 hasConceptScore W2938597352C178790620 @default.
- W2938597352 hasConceptScore W2938597352C179104552 @default.
- W2938597352 hasConceptScore W2938597352C181199279 @default.
- W2938597352 hasConceptScore W2938597352C185592680 @default.
- W2938597352 hasConceptScore W2938597352C192989942 @default.
- W2938597352 hasConceptScore W2938597352C24840226 @default.
- W2938597352 hasConceptScore W2938597352C2775832776 @default.
- W2938597352 hasConceptScore W2938597352C2776217839 @default.
- W2938597352 hasConceptScore W2938597352C2778824058 @default.
- W2938597352 hasConceptScore W2938597352C2781170380 @default.
- W2938597352 hasConceptScore W2938597352C2900893 @default.
- W2938597352 hasConceptScore W2938597352C48843007 @default.
- W2938597352 hasConceptScore W2938597352C523546767 @default.
- W2938597352 hasConceptScore W2938597352C54355233 @default.
- W2938597352 hasConceptScore W2938597352C55493867 @default.
- W2938597352 hasConceptScore W2938597352C55904794 @default.
- W2938597352 hasConceptScore W2938597352C62231903 @default.
- W2938597352 hasConceptScore W2938597352C75473681 @default.
- W2938597352 hasConceptScore W2938597352C86803240 @default.
- W2938597352 hasLocation W29385973521 @default.
- W2938597352 hasOpenAccess W2938597352 @default.
- W2938597352 hasPrimaryLocation W29385973521 @default.
- W2938597352 hasRelatedWork W103356491 @default.
- W2938597352 hasRelatedWork W1780994009 @default.
- W2938597352 hasRelatedWork W1976721778 @default.
- W2938597352 hasRelatedWork W1994193521 @default.
- W2938597352 hasRelatedWork W2012635273 @default.
- W2938597352 hasRelatedWork W2033123336 @default.
- W2938597352 hasRelatedWork W2104785052 @default.
- W2938597352 hasRelatedWork W2132301901 @default.
- W2938597352 hasRelatedWork W2247335314 @default.
- W2938597352 hasRelatedWork W2467634136 @default.
- W2938597352 hasRelatedWork W2621127270 @default.
- W2938597352 hasRelatedWork W2783647467 @default.
- W2938597352 hasRelatedWork W2787389272 @default.
- W2938597352 hasRelatedWork W2923676638 @default.
- W2938597352 hasRelatedWork W2945979259 @default.
- W2938597352 hasRelatedWork W2952852654 @default.
- W2938597352 hasRelatedWork W3003581228 @default.
- W2938597352 hasRelatedWork W3108724245 @default.
- W2938597352 hasRelatedWork W3120128391 @default.
- W2938597352 hasRelatedWork W70550605 @default.
- W2938597352 isParatext "false" @default.
- W2938597352 isRetracted "false" @default.
- W2938597352 magId "2938597352" @default.
- W2938597352 workType "dissertation" @default.