Matches in SemOpenAlex for { <https://semopenalex.org/work/W2938796543> ?p ?o ?g. }
- W2938796543 abstract "Little is known about how food interacts with the intestinal epithelium during the digestion process. However, it is known that ingredients in food can modulate the intestinal barrier, and have the potential to disrupt homeostasis of the gut. Here, we characterise a conditionally immortalised canine intestinal epithelial cell (cIEC) line for use in in vitro assays, to assess the effect of food ingredients on intestinal barrier function, permeability, cell health, and inflammation. Microscopy and flow cytometry confirmed that cIECs had a phenotype consistent with those of epithelial origin, and were able to differentiate to mature enterocytes. The cIECs also formed a monolayer when grown on Transwell® inserts, producing functional tight junctions between the cells. In contrast to the human-derived Caco-2 cell line, transepithelial electrical resistance (TEER) was increased in cIECs in response to two different raw ingredients. The exposure of cIECs to known inflammatory stimuli and raw ingredients induced the nuclear translocation of nuclear factor kappa-light-chain-enhancer of activated B-cells (NF-κB). This work demonstrates the value of a unique cIEC in vitro model to study the effects of food ingredients on canine intestinal function and health, and supports continued efforts to reduce and refine the use of animals in scientific research." @default.
- W2938796543 created "2019-04-25" @default.
- W2938796543 creator A5020473619 @default.
- W2938796543 creator A5040133703 @default.
- W2938796543 creator A5047937571 @default.
- W2938796543 creator A5055680335 @default.
- W2938796543 creator A5068357203 @default.
- W2938796543 date "2018-07-01" @default.
- W2938796543 modified "2023-09-25" @default.
- W2938796543 title "Characterisation of a Canine Epithelial Cell Line for Modelling the Intestinal Barrier" @default.
- W2938796543 cites W1512595753 @default.
- W2938796543 cites W1556702215 @default.
- W2938796543 cites W1730252720 @default.
- W2938796543 cites W1847231704 @default.
- W2938796543 cites W1927073913 @default.
- W2938796543 cites W1973399759 @default.
- W2938796543 cites W1973488401 @default.
- W2938796543 cites W1979051583 @default.
- W2938796543 cites W1984172120 @default.
- W2938796543 cites W1985106325 @default.
- W2938796543 cites W2002131773 @default.
- W2938796543 cites W2015734787 @default.
- W2938796543 cites W2019284666 @default.
- W2938796543 cites W2020734315 @default.
- W2938796543 cites W2021961503 @default.
- W2938796543 cites W2032470192 @default.
- W2938796543 cites W2035788923 @default.
- W2938796543 cites W2041454039 @default.
- W2938796543 cites W2048715361 @default.
- W2938796543 cites W2050054338 @default.
- W2938796543 cites W2050953850 @default.
- W2938796543 cites W2055959752 @default.
- W2938796543 cites W2058557855 @default.
- W2938796543 cites W2060013640 @default.
- W2938796543 cites W2069793009 @default.
- W2938796543 cites W2070305054 @default.
- W2938796543 cites W207915311 @default.
- W2938796543 cites W2085091748 @default.
- W2938796543 cites W2085248976 @default.
- W2938796543 cites W2095147460 @default.
- W2938796543 cites W2111708038 @default.
- W2938796543 cites W2112478001 @default.
- W2938796543 cites W2113572311 @default.
- W2938796543 cites W2114509412 @default.
- W2938796543 cites W2114560503 @default.
- W2938796543 cites W2120046045 @default.
- W2938796543 cites W2122341732 @default.
- W2938796543 cites W2129712974 @default.
- W2938796543 cites W2130501069 @default.
- W2938796543 cites W2132935604 @default.
- W2938796543 cites W2133464603 @default.
- W2938796543 cites W2134354994 @default.
- W2938796543 cites W2137841218 @default.
- W2938796543 cites W2138024379 @default.
- W2938796543 cites W2143296478 @default.
- W2938796543 cites W2151502111 @default.
- W2938796543 cites W2151866033 @default.
- W2938796543 cites W2157313121 @default.
- W2938796543 cites W2161751637 @default.
- W2938796543 cites W2171148360 @default.
- W2938796543 cites W2297400589 @default.
- W2938796543 cites W2304397752 @default.
- W2938796543 cites W2329862917 @default.
- W2938796543 cites W2346113929 @default.
- W2938796543 cites W2490544534 @default.
- W2938796543 doi "https://doi.org/10.1177/026119291804600304" @default.
- W2938796543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30022673" @default.
- W2938796543 hasPublicationYear "2018" @default.
- W2938796543 type Work @default.
- W2938796543 sameAs 2938796543 @default.
- W2938796543 citedByCount "2" @default.
- W2938796543 countsByYear W29387965432020 @default.
- W2938796543 countsByYear W29387965432022 @default.
- W2938796543 crossrefType "journal-article" @default.
- W2938796543 hasAuthorship W2938796543A5020473619 @default.
- W2938796543 hasAuthorship W2938796543A5040133703 @default.
- W2938796543 hasAuthorship W2938796543A5047937571 @default.
- W2938796543 hasAuthorship W2938796543A5055680335 @default.
- W2938796543 hasAuthorship W2938796543A5068357203 @default.
- W2938796543 hasConcept C126322002 @default.
- W2938796543 hasConcept C153911025 @default.
- W2938796543 hasConcept C177779419 @default.
- W2938796543 hasConcept C185592680 @default.
- W2938796543 hasConcept C193176779 @default.
- W2938796543 hasConcept C202751555 @default.
- W2938796543 hasConcept C2781077229 @default.
- W2938796543 hasConcept C3019368612 @default.
- W2938796543 hasConcept C51911345 @default.
- W2938796543 hasConcept C529295009 @default.
- W2938796543 hasConcept C54355233 @default.
- W2938796543 hasConcept C553184892 @default.
- W2938796543 hasConcept C55493867 @default.
- W2938796543 hasConcept C71924100 @default.
- W2938796543 hasConcept C81885089 @default.
- W2938796543 hasConcept C86803240 @default.
- W2938796543 hasConcept C95444343 @default.
- W2938796543 hasConceptScore W2938796543C126322002 @default.
- W2938796543 hasConceptScore W2938796543C153911025 @default.
- W2938796543 hasConceptScore W2938796543C177779419 @default.
- W2938796543 hasConceptScore W2938796543C185592680 @default.