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- W2939267646 abstract "Abstract Studies on biological functions of N 6 -methyladenosine (m 6 A) modification in mRNA have sprung up in recent years. Here we construct and characterize a CRISPR-Cas13b-based tool for the first time that targeted m 6 A methylation of mRNA by fusing the catalytically dead Type VI-B Cas13 enzyme from Prevotella sp.P5-125 (dPspCas13b) with the m 6 A demethylase ALKBH5, which is named as dm 6 ACRISPR. Subsequently, such system is shown to specific demethylase the m 6 A of target mRNA such as CYB5A to increase its mRNA stability. In addition, the dm 6 ACRISPR system appeared to afford efficient demethylation of the target genes with tenuous off-target effects. Together, we provide a programmable and in vivo manipulation tool to study mRNA modification and its potential biological functions of specific gene." @default.
- W2939267646 created "2019-04-25" @default.
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- W2939267646 date "2019-04-20" @default.
- W2939267646 modified "2023-09-27" @default.
- W2939267646 title "Targeted mRNA demethylation using an engineered dCas13b-ALKBH5 fusion protein" @default.
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- W2939267646 doi "https://doi.org/10.1101/614859" @default.
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