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- W2939457956 abstract "Present study focused on evaluation of the effect of national nancomposite product on serum biochemical profile andprogrammed cell death (apoptosis) by using Annexin V-FITC and PI test of breast carcinoma female rats. Basic nanocurcuminwas tested for its protective effect on breast cancer female rats (n= 30) divided in two main groups (NTBR) and(TBR) which in turn classified into (TBR- Control Positive and TBR- basic nano- curcumin protective groups). Basicnano-curcumin was formulated and tested for both IC50 (IC50 = 1.0 μg) and LD5o (35 mg/kg by wt.) by using in vitro andin vivo techniques, respectively. Breast cells apoptosis was determined by Annexin V-FITC, which is a fluorescent probebinds to phosphotidylserine (PS) in a Ca 2+ dependent pathway. Breast Cells were stained with Annexin V and PI. Theapoptotic cells [Annexin (+) and PI (-)] were differentiated from necrotic cells [Annexin (-) and PI (+)] using flowcytometry technique. The anti-proliferative effect of basic nano-curcumin appeared much earlier due to its nano form.Also, Liver [ALT (40U/l), AST (85 U/l)] and kidney [BUN (19 mg/dl), Creatinine (0.5 mg/dl)] functions were decreasedwithin normal ranges" @default.
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- W2939457956 date "2017-12-01" @default.
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- W2939457956 title "Protective role of new nanocomposite natural product (basic nano-curcumin) against breast carcinoma" @default.
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- W2939457956 doi "https://doi.org/10.21608/bvmj.2017.30006" @default.
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