FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2939479969> ?p ?o ?g. }

• W2939479969 endingPage "141" @default.
• W2939479969 startingPage "133" @default.
• W2939479969 abstract "Fatty acid desaturases (Fads) are a key enzyme in the process of biosynthesis of highly unsaturated fatty acids (HUFAs). In this study, we cloned the full-length sequence of the SiFad1 gene (SiFad1) and analyzed its expression profiles during different developmental stages and in different tissues of Strongylocentrotus intermedius. The full-length cDNA of SiFad1 is composed of 1086 bp, with a putative open reading frame of 885 bp encoding a polypeptide of 294 amino acid (AA) residues. The predicted molecular mass of SiFad1 is 34.67 kDa and its theoretical pI is 8.41. The presence of conserved motifs including three histidine boxes (HXXXH, HXXHH, XXXHH), a FA_desaturases domain and three transmembrane domains suggests that SiFad1 belongs to the microsomal fatty acid desaturases family. Its tissue distribution showed that the highest expression of SiFad1 is in the intestine and the weakest expression is in Aristotle's lantern of S. intermedius. Time-course expression measurements in different developmental stages showed the highest expression of SiFad1 occurs in the gastrula and the weakest expression in the juvenile sea urchin. Knock-down of SiFad1 by specific siRNA revealed that the significantly depressed expression of Elovl5 had decreased in the coelomocytes, intestines and gonads at 24 h post transfection, indicating that the downstream target gene of SiFad1 is Elovl5 and SiFad1 and Elovl5 have positive regulatory effects. When we examined the changes in fatty acids in the gonads before and after interference, the results showed that after 24 h of interference, the content of C20:4n-6 produced by SiFad1 had decreased. Taken together, these results will enable us to understand the role of SiFad1 in fatty acid anabolism, which will help us to understand the fatty acid synthesis pathways and regulatory mechanisms of Strongylocentrotus intermedius and provide a theoretical experimental basis for improving the ability of sea urchins to synthesize fatty acids and cultivating sea urchins of higher quality and nutritional value." @default.
• W2939479969 created "2019-04-25" @default.
• W2939479969 creator A5005303855 @default.
• W2939479969 creator A5009810312 @default.
• W2939479969 creator A5056874237 @default.
• W2939479969 creator A5060071396 @default.
• W2939479969 creator A5060245615 @default.
• W2939479969 creator A5062695779 @default.
• W2939479969 creator A5085331622 @default.
• W2939479969 creator A5091753120 @default.
• W2939479969 date "2019-07-01" @default.
• W2939479969 modified "2023-10-06" @default.
• W2939479969 title "Molecular characterization and expression of SiFad1 in the sea urchin (Strongylocentrotus intermedius)" @default.
• W2939479969 cites W1966151581 @default.
• W2939479969 cites W1967108842 @default.
• W2939479969 cites W1974401020 @default.
• W2939479969 cites W1981713807 @default.
• W2939479969 cites W1990876848 @default.
• W2939479969 cites W1992363218 @default.
• W2939479969 cites W1998066116 @default.
• W2939479969 cites W1999879148 @default.
• W2939479969 cites W1999919904 @default.
• W2939479969 cites W2007711401 @default.
• W2939479969 cites W2011101920 @default.
• W2939479969 cites W2012216316 @default.
• W2939479969 cites W2013357109 @default.
• W2939479969 cites W2016998569 @default.
• W2939479969 cites W2019051953 @default.
• W2939479969 cites W2021805559 @default.
• W2939479969 cites W2029598860 @default.
• W2939479969 cites W2036303685 @default.
• W2939479969 cites W2036539947 @default.
• W2939479969 cites W2055099754 @default.
• W2939479969 cites W2056943679 @default.
• W2939479969 cites W2059483997 @default.
• W2939479969 cites W2062551414 @default.
• W2939479969 cites W2070266571 @default.
• W2939479969 cites W2070961413 @default.
• W2939479969 cites W2081493241 @default.
• W2939479969 cites W2091532861 @default.
• W2939479969 cites W2107261609 @default.
• W2939479969 cites W2107277218 @default.
• W2939479969 cites W2146814107 @default.
• W2939479969 cites W2146863574 @default.
• W2939479969 cites W2152011621 @default.
• W2939479969 cites W2154061926 @default.
• W2939479969 cites W2167075471 @default.
• W2939479969 cites W2167413855 @default.
• W2939479969 cites W2225837507 @default.
• W2939479969 cites W2257150068 @default.
• W2939479969 cites W2312348112 @default.
• W2939479969 cites W2318357320 @default.
• W2939479969 cites W2339709423 @default.
• W2939479969 cites W2380957411 @default.
• W2939479969 cites W2509241206 @default.
• W2939479969 cites W2568019981 @default.
• W2939479969 cites W2737267157 @default.
• W2939479969 cites W2782810157 @default.
• W2939479969 doi "https://doi.org/10.1016/j.gene.2019.04.043" @default.
• W2939479969 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31004713" @default.
• W2939479969 hasPublicationYear "2019" @default.
• W2939479969 type Work @default.
• W2939479969 sameAs 2939479969 @default.
• W2939479969 citedByCount "15" @default.
• W2939479969 countsByYear W29394799692019 @default.
• W2939479969 countsByYear W29394799692020 @default.
• W2939479969 countsByYear W29394799692021 @default.
• W2939479969 countsByYear W29394799692022 @default.
• W2939479969 countsByYear W29394799692023 @default.
• W2939479969 crossrefType "journal-article" @default.
• W2939479969 hasAuthorship W2939479969A5005303855 @default.
• W2939479969 hasAuthorship W2939479969A5009810312 @default.
• W2939479969 hasAuthorship W2939479969A5056874237 @default.
• W2939479969 hasAuthorship W2939479969A5060071396 @default.
• W2939479969 hasAuthorship W2939479969A5060245615 @default.
• W2939479969 hasAuthorship W2939479969A5062695779 @default.
• W2939479969 hasAuthorship W2939479969A5085331622 @default.
• W2939479969 hasAuthorship W2939479969A5091753120 @default.
• W2939479969 hasConcept C104317684 @default.
• W2939479969 hasConcept C150194340 @default.
• W2939479969 hasConcept C153911025 @default.
• W2939479969 hasConcept C167625842 @default.
• W2939479969 hasConcept C187882448 @default.
• W2939479969 hasConcept C19924922 @default.
• W2939479969 hasConcept C2776908312 @default.
• W2939479969 hasConcept C2778460671 @default.
• W2939479969 hasConcept C47289529 @default.
• W2939479969 hasConcept C515207424 @default.
• W2939479969 hasConcept C543025807 @default.
• W2939479969 hasConcept C55493867 @default.
• W2939479969 hasConcept C86803240 @default.
• W2939479969 hasConcept C95444343 @default.
• W2939479969 hasConceptScore W2939479969C104317684 @default.
• W2939479969 hasConceptScore W2939479969C150194340 @default.
• W2939479969 hasConceptScore W2939479969C153911025 @default.
• W2939479969 hasConceptScore W2939479969C167625842 @default.
• W2939479969 hasConceptScore W2939479969C187882448 @default.
• W2939479969 hasConceptScore W2939479969C19924922 @default.

• next