FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2939712269> ?p ?o ?g. }

• W2939712269 endingPage "1231" @default.
• W2939712269 startingPage "1221" @default.
• W2939712269 abstract "Purpose: Aloin (ALO), a bioactive ingredient extracted from aloe vera, has anti-tumor effects. High Mobility Group Box 1 (HMGB1), a highly conserved nuclear DNA-binding protein, has been implicated in various cancer types. Highly expressed HMGB1 is closely associated with tumor cells apoptosis, proliferation and migration. We investigated the specific molecular mechanisms by which ALO-induced apoptosis by targeting HMGB1 in gastric cancer cells. Materials and methods: Human gastric cancer HGC-27 cells were treated with different doses of ALO (100, 200 and 400 µg/ml) for 24 h, after which DAPI staining was used to observe the nuclear morphology, Annexin V/PI double staining assay was used to determine the rate of apoptosis; Western blotting was used to detect the levels of PARP, pro-caspase3, HMGB1 and RAGE; nuclear translocation of HMGB1 was determined by conducting a nucleoplasm separation experiment. The Enzyme linked immunosorbent assay (ELISA) assay was used to detect release of HMGB1. The HGC-27 cells, transfected with HMGB1 shRNA plasmids, were stimulated with ALO for 24 h, after which a flow cytometry assay was used to detect the rate of apoptosis. HGC-27 cells were pre-treated with or without ALO and then stimulated with rhHMGB1, the phosphorylation of Akt, mTOR, P70S6K, S6, 4EBP1, ERK, P90RSK, cAMP regulatory element binding (CREB) were detected by Western blotting. Results: After different doses of ALO treatment, the nuclei showed morphological changes characteristic of apoptosis. Apoptotic rates were enhanced in a dose dependent manner. The level of cleaved PARP was enhanced and pro-caspase3, HMGB1 and RAGE levels were reduced, HMGB1 nuclear translocation and release were inhibited. The activation of rhHMGB1-induced Akt-mTOR-P70S6K and ERK-CREB signalling pathways was inhibited by ALO. Blocking these signalling pathways by special inhibitors and HMGB1 knockdown could enhance ALO-induced HGC-27 cell apoptosis. Conclusion: ALO- induced HGC-27 cell apoptosis by down-regulating expressions of HMGB1 and RAGE, inhibiting HMGB1 release and then suppressing rhHMGB1-induced activation of Akt-mTOR-P70S6K and ERK-P90RSK-CREB signalling pathways." @default.
• W2939712269 created "2019-04-25" @default.
• W2939712269 creator A5004831082 @default.
• W2939712269 creator A5010215768 @default.
• W2939712269 creator A5016806891 @default.
• W2939712269 creator A5019210915 @default.
• W2939712269 creator A5053956248 @default.
• W2939712269 creator A5060246593 @default.
• W2939712269 creator A5061902605 @default.
• W2939712269 creator A5066822151 @default.
• W2939712269 creator A5071535153 @default.
• W2939712269 creator A5080577523 @default.
• W2939712269 date "2019-04-01" @default.
• W2939712269 modified "2023-09-30" @default.
• W2939712269 title "<p>The molecular mechanisms of Aloin induce gastric cancer cells apoptosis by targeting High Mobility Group Box 1</p>" @default.
• W2939712269 cites W1068493845 @default.
• W2939712269 cites W1531688131 @default.
• W2939712269 cites W1902173561 @default.
• W2939712269 cites W1967531551 @default.
• W2939712269 cites W1982096171 @default.
• W2939712269 cites W1983348216 @default.
• W2939712269 cites W1997956758 @default.
• W2939712269 cites W2020323703 @default.
• W2939712269 cites W2052094075 @default.
• W2939712269 cites W2106039427 @default.
• W2939712269 cites W2116691487 @default.
• W2939712269 cites W2126775001 @default.
• W2939712269 cites W2145541777 @default.
• W2939712269 cites W2148198816 @default.
• W2939712269 cites W2153747194 @default.
• W2939712269 cites W2285911646 @default.
• W2939712269 cites W2321116474 @default.
• W2939712269 cites W2614770255 @default.
• W2939712269 cites W2753432612 @default.
• W2939712269 cites W2801255229 @default.
• W2939712269 cites W2804156286 @default.
• W2939712269 cites W2809783562 @default.
• W2939712269 cites W2884001293 @default.
• W2939712269 cites W2884425950 @default.
• W2939712269 cites W2893742563 @default.
• W2939712269 cites W2894444025 @default.
• W2939712269 cites W2896513495 @default.
• W2939712269 cites W2901616455 @default.
• W2939712269 cites W2995631245 @default.
• W2939712269 cites W3024396134 @default.
• W2939712269 cites W3025493238 @default.
• W2939712269 doi "https://doi.org/10.2147/dddt.s201818" @default.
• W2939712269 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6489572" @default.
• W2939712269 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31114162" @default.
• W2939712269 hasPublicationYear "2019" @default.
• W2939712269 type Work @default.
• W2939712269 sameAs 2939712269 @default.
• W2939712269 citedByCount "23" @default.
• W2939712269 countsByYear W29397122692020 @default.
• W2939712269 countsByYear W29397122692021 @default.
• W2939712269 countsByYear W29397122692022 @default.
• W2939712269 countsByYear W29397122692023 @default.
• W2939712269 crossrefType "journal-article" @default.
• W2939712269 hasAuthorship W2939712269A5004831082 @default.
• W2939712269 hasAuthorship W2939712269A5010215768 @default.
• W2939712269 hasAuthorship W2939712269A5016806891 @default.
• W2939712269 hasAuthorship W2939712269A5019210915 @default.
• W2939712269 hasAuthorship W2939712269A5053956248 @default.
• W2939712269 hasAuthorship W2939712269A5060246593 @default.
• W2939712269 hasAuthorship W2939712269A5061902605 @default.
• W2939712269 hasAuthorship W2939712269A5066822151 @default.
• W2939712269 hasAuthorship W2939712269A5071535153 @default.
• W2939712269 hasAuthorship W2939712269A5080577523 @default.
• W2939712269 hasBestOaLocation W29397122691 @default.
• W2939712269 hasConcept C104317684 @default.
• W2939712269 hasConcept C121608353 @default.
• W2939712269 hasConcept C153911025 @default.
• W2939712269 hasConcept C170493617 @default.
• W2939712269 hasConcept C182979987 @default.
• W2939712269 hasConcept C185592680 @default.
• W2939712269 hasConcept C190283241 @default.
• W2939712269 hasConcept C2777527632 @default.
• W2939712269 hasConcept C2780545709 @default.
• W2939712269 hasConcept C502942594 @default.
• W2939712269 hasConcept C54009773 @default.
• W2939712269 hasConcept C54355233 @default.
• W2939712269 hasConcept C552990157 @default.
• W2939712269 hasConcept C553184892 @default.
• W2939712269 hasConcept C55493867 @default.
• W2939712269 hasConcept C75217442 @default.
• W2939712269 hasConcept C82381507 @default.
• W2939712269 hasConcept C85265743 @default.
• W2939712269 hasConcept C86554907 @default.
• W2939712269 hasConcept C86803240 @default.
• W2939712269 hasConcept C88634738 @default.
• W2939712269 hasConcept C96232424 @default.
• W2939712269 hasConceptScore W2939712269C104317684 @default.
• W2939712269 hasConceptScore W2939712269C121608353 @default.
• W2939712269 hasConceptScore W2939712269C153911025 @default.
• W2939712269 hasConceptScore W2939712269C170493617 @default.
• W2939712269 hasConceptScore W2939712269C182979987 @default.
• W2939712269 hasConceptScore W2939712269C185592680 @default.
• W2939712269 hasConceptScore W2939712269C190283241 @default.

• next